The influence of sun exposure on the DNA methylation status of MMP9, miR-137, KRT14 and KRT19 genes in human skin

Melo, Alanne Rayssa da Silva; Barroso, Haline; Araújo, Daniel Uchôa De; Pereira, Francisco Ruidomar; Oliveira, Naila Francis Paulo de

doi:10.1684/ejd.2015.2598

Cited by 11 publications

(13 citation statements)

References 47 publications

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“…Da Silva Melo et al . investigated the sun‐exposure influence on the DNA methylation profile of ageing‐related genes in skin cells, including matrix metalloprotease‐9 ( MMP9 ), microRNA 137 ( MIR137 ), cytokeratin 14 ( KRT14 ) and cytokeratin 19 ( KRT19 ) . They found that sun exposure did not induce changes in the DNA methylation status in these four genes.…”

Section: Discussionmentioning

confidence: 99%

Chronic sun exposure is associated with distinct histone acetylation changes in human skin

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Chronic sun exposure is associated with distinct histone acetylation changes in human skin

et al. 2018

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“…Genome-wide DNA methylation analysis of mouse skin and human fibroblasts showed that aging induces both hypermethylation and hypomethylation in the promoters of various genes (Koch et al, 2011;Qian and Xu, 2014). However, sun exposure did not affect DNA methylation of MMP-9, keratin 14, and keratin 19 in human skin in a previous study (Da Silva Melo et al, 2015). Although a relatively stable methylation pattern was found in the aged human epidermis (Gronniger et al, 2010;Raddatz et al, 2013) and in human keratinocytes (Lahtz et al, 2013), a more recent report showed widespread blocks of hypomethylation in older, sun-exposed epidermis of human skin but failed to delineate the differential methylome in the dermis (Vandiver et al, 2015).…”

Section: Discussionmentioning

confidence: 81%

Inhibition of DNA Methylation in the COL1A2 Promoter by Anacardic Acid Prevents UV-Induced Decrease of Type I Procollagen Expression

Kim

Cheng

et al. 2017

Journal of Investigative Dermatology

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UV radiation decreases type I procollagen production mainly by inhibiting the transforming growth factor-β/Smad signaling pathway. Because further epigenetic regulatory mechanisms are unclear, we investigated the roles of DNA methylation and histone acetylation in UV-induced regulation of COL1A2 transcription in human dermal fibroblasts. Anacardic acid, a p300 histone acetyltransferase inhibitor, rescued the UV-induced decrease of type I procollagen expression in human dermal fibroblasts. Although UV irradiation induced global histone acetylation, it reduced the local recruitment of histone H3 acetylation as well as p300, and Smad2/3 to the p300 binding site (-1406/-1393), in the COL1A2 promoter as shown by chromatin immunoprecipitation. This effect was reversed by anacardic acid treatment. In contrast, pyrosequencing analysis showed that UV irradiation induced DNA methylation in the same region of the COL1A2 promoter, which was reversed by anacardic acid and a DNA methyltransferase inhibitor (5-AZA-2'-deoxycytidine). Inhibition of UV-induced DNA methylation led to an increase of UV-induced histone acetylation in the COL1A2 promoter and increased the recruitment of transcription factors, leading to up-regulation of type I collagen after UV irradiation. Collectively, our findings indicate that the epigenetic crosstalk between DNA methylation and histone acetylation plays a crucial role in COL1A2 transcription induced by UV irradiation.

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“…Our group has previously demonstrated that the methylated profile of miR-137 in skin samples is also associated with the female gender. 44 In fact, the female gender has proven to be positively associated with DNA methylation for some genes . Here, we also detected hypermethylation in global methylation, which could be associated with aging (menopause), as discussed above.…”

Section: Discussionmentioning

confidence: 99%

“…This is based on evidence that in addition to the four different miR-9-1 methylation profiles among the individuals analysed in the present study, there were five different miR-137 methylation profiles previously observed in a sample of 30 individuals. 44 Therefore, epigenome profiles can vary both among various cell types and within a particular individual, which may then lead to unique epigenome profiles associated with a particular disease state. This finding reinforces the issue of the significant influence of intrinsic and extrinsic factors on epigenetic profiles, and it should be used as a basis for the individualization of drug therapies.…”

Section: Discussionmentioning

confidence: 99%

“…Our data shows that sunlight exposure does not influence the DNA methylation profiles of the miR-9-3 and MTHFR genes based on the similar frequencies of the unmethylated and methylated conditions for both skin areas for miR-9-3 and MTHFR, respectively ( Figure 2 ).The high frequencies of those profiles show that age, gender and skin type also do not influence the methylation profile of these genes. Although some studies show a correlation with age and gender 44 in skin tissue, it is known that these changes occur in a very specific way, that is, some genes are more susceptible than others to these factors. 29 , 32 , 40 , 44 …”

Section: Discussionmentioning

confidence: 99%

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Global and gene-specific DNA methylation and hydroxymethylation in human skin exposed and not exposed to sun radiation

Silva

Melo

Costa

et al. 2017

An. Bras. Dermatol.

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Backgroundepigenomes can be influenced by environmental factors leading to the development of diseases.ObjectiveTo investigate the influence of sun exposure on global DNA methylation and hydroxymethylation status and at specific sites of the miR-9-1, miR-9-3 and MTHFR genes in skin samples of subjects with no history of skin diseases.MethodsSkin samples were obtained by punch on sun-exposed and sun-protected arm areas from 24 corpses of 16-89 years of age. Genomic DNA was extracted from skin samples that were ranked according to Fitzpatrick's criteria as light, moderate, and dark brown. Global DNA methylation and hydroxymethylation and DNA methylation analyses at specific sites were performed using ELISA and MSP, respectively.ResultsNo significant differences in global DNA methylation and hydroxymethylation levels were found among the skin areas, skin types, or age. However, gender-related differences were detected, where women showed higher methylation levels. Global DNA methylation levels were higher than hydroxymethylation levels, and the levels of these DNA modifications correlated in skin tissue. For specific sites, no differences among the areas were detected. Additional analyses showed no differences in the methylation status when age, gender, and skin type were considered; however, the methylation status of the miR-9-1 gene seems to be gender related.Study limitationsthere was no separation of dermis and epidermis and low sample size.Conclusionsun exposure does not induce changes in the DNA methylation and hydroxymethylation status or in miR-9-1, miR-9-3 and MTHFR genes for the studied skin types.

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The influence of sun exposure on the DNA methylation status of MMP9, miR-137, KRT14 and KRT19 genes in human skin

Cited by 11 publications

References 47 publications

Chronic sun exposure is associated with distinct histone acetylation changes in human skin

Chronic sun exposure is associated with distinct histone acetylation changes in human skin

Inhibition of DNA Methylation in the COL1A2 Promoter by Anacardic Acid Prevents UV-Induced Decrease of Type I Procollagen Expression

Global and gene-specific DNA methylation and hydroxymethylation in human skin exposed and not exposed to sun radiation

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