The influence of substrate systems on the enantioselective and lipolytic activity of immobilized Amano PS from Burkholderia cepacia lipase (APS-BCL)

Dulęba, Jacek; Siódmiak, Tomasz; Marszałł, Michał Piotr

doi:10.1016/j.procbio.2022.06.003

Cited by 9 publications

(16 citation statements)

References 104 publications

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“…In turn, with respect to the immobilization efficiency parameter, immobilized AA-ANL was the most active in pumpkin seed (2400.00%), fish (1433.33%), and corn (1266.67%) oils. A comparison of the obtained results with literature data [62] suggests that immobilization significantly improved the lipolytic activity of AA-ANL. Lipase APS-BCL hyperactivation occurred in four oils (peanut, pumpkin seed, walnut, and corn oils), as expressed by activity recovery, whereas hyperactivation expressed by activity retention was only demonstrated for peanut oil.…”

Section: Effect Of Substrates On the Lipolytic Activity Of Immobilize...supporting

confidence: 53%

“…Hence, unsaturated fatty acids are applied in developed multicomponent (MC-UFA) systems. As suggested by the authors of previous works [61,62], the ω6/ω3 PUFA supplementation system seems to be the most beneficial for the human organism. In contrast, the proportion of ω6 to ω3 PUFAs should not be high due to the previously mentioned risk of obesity associated with fatty liver [73].…”

Section: Or Peer Reviewmentioning

confidence: 74%

“…The application of triglycerides containing fatty acids is determined by the length of the carbon chain appropriate for lipase specificity, e.g., CAL-B prefers short-chain fatty acids. In contrast, lipase APS-BCL shows high activity toward carbon chains, regardless of their length [10,61,62]. In turn, AA-ANL prefers substrates containing medium-length carbon chains [24].…”

Section: Or Peer Reviewmentioning

confidence: 95%

“…They are an important component of a healthy human diet, especially EPA and DHA, the supplementation of which decreases cardiovascular disease risk. Additionally, they exert a slight antidepressive effect and support lipogenesis, which is a promising obesity treatment [62][63][64][65][66][67]. According to recent reports, ω3 PUFA supplementation, especially DHA, can accelerate the regeneration of the organism after COVID-19 infection [68,69].…”

Section: Or Peer Reviewmentioning

confidence: 99%

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The High ‘Lipolytic Jump’ of Immobilized Amano A Lipase from Aspergillus niger in Developed ‘ESS Catalytic Triangles’ Containing Natural Origin Substrates

et al. 2022

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Lipase Amano A from Aspergillus niger (AA-ANL) is among the most commonly applied enzymes in biocatalysis processes, making it a significant scientific subject in the pharmaceutical and medical disciplines. In this study, we investigated the lipolytic activity of AA-ANL immobilized onto polyacrylic support IB-150A in 23 oils of natural origin containing various amounts of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs). The created systems were expressed as an ‘ESS catalytic triangle’. A distinct ‘jump’ (up to 2400%) of lipolytic activity of immobilized AA-ANL compared to free lipase and hyperactivation in mostly tested substrates was observed. There was a ‘cutoff limit’ in a quantitative mutual ratio of ω-PUFAs/MUFAs, for which there was an increase or decrease in the activity of the immobilized AA-ANL. In addition, we observed the beneficial effect of immobilization using three polyacrylic supports (IB-150A, IB-D152, and IB-EC1) characterized by different intramolecular interactions. The developed substrate systems demonstrated considerable hyperactivation of immobilized AA-ANL. Moreover, a ‘lipolytic jump’ in the full range of tested temperature and pH was also observed. The considerable activity of AA-ANL-IB-150A after four reuse cycles was demonstrated. On the other hand, we observed an essential decrease in stability of immobilized lipase after 168 h of storage in a climate chamber. The tested kinetic profile of immobilized AA-ANL confirmed the increased affinity to the substrate relative to lipase in the free form.

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Section: Effect Of Substrates On the Lipolytic Activity Of Immobilize...supporting

confidence: 53%

Section: Or Peer Reviewmentioning

confidence: 74%

Section: Or Peer Reviewmentioning

confidence: 95%

Section: Or Peer Reviewmentioning

confidence: 99%

See 2 more Smart Citations

The High ‘Lipolytic Jump’ of Immobilized Amano A Lipase from Aspergillus niger in Developed ‘ESS Catalytic Triangles’ Containing Natural Origin Substrates

et al. 2022

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“…The lipases of Pseudomonas Cepacia have a highly open configuration [ 32 , 33 ], and therefore the substrate with which it interacts must have the sites with the appropriate conformation that facilitates its anchoring. The lipase of Pseudomonas Cepacia is characterized by presenting thermal resistance and tolerance to various substances, especially alcohols [ 34 ]. If we add to these properties a good anchoring of this enzyme, its thermal resistance would be increased, and therefore the resulting biocatalysts can be used in transesterification reactions that exceed the thermal limits of free lipase [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Biocatalysts Synthesized with Lipase from Pseudomonas cepacia on Glycol-Modified ZIF-8: Characterization and Utilization in the Synthesis of Green Biodiesel

et al. 2022

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This research presents results on the production of biodiesel from the transesterification of acylglycerides present in palm oil, using the biocatalysts ZIF-8-PCL and Gly@ZIF-8-PCL synthesized by immobilization of Pseudomonas Cepacia Lipase as catalytic materials and using pure ZIF-8 and Gly@ZIF-8 (modified ZIF-8) as supports. The Gly@ZIF-8 carbonaceous material was prepared by wet impregnation of ZIF-8 with ethylene glycol as the carbon source, and then thermally modified. The calcination conditions were 900 °C for two hours with a heating rate of 7 °C/min in an inert atmosphere. A textural characterization was performed, and results showed superficial changes of materials at the microporous and mesoporous levels for the Gly@ZIF-8 material. Both the starting materials and biocatalysts were characterized by infrared spectroscopy (FTIR) and Raman spectroscopy. During the transesterification, using the two biocatalysts (ZIF-8-PCL and Gly@ZIF-8-PCL), two supernatant liquids were generated which were characterized by infrared spectroscopy (FTIR), gas chromatography coupled to mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The results show that the two routes of synthesis of supports from ZIF-8 will be configured as effective methods for the generation of effective biocatalysts for biodiesel production.

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Exploring fatty acid composition, bioactive compounds, and antioxidant properties in oils of newly developed sesame mutant lines in Morocco

Kouighat,

Guirrou,

El Antari

et al. 2024

J Americ Oil Chem Soc

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Sesame (Sesamum indicum L.) is renowned for its significance as a global oilseed crop, valued for its high oil content and nutritional properties. This study aimed to explore the fatty acid composition, bioactive compounds, and antioxidant attributes of sesame mutant oils, examining their potential applications in food industry. Nine M4 mutants, along with their parents, were grown in two contrasting environments (Taoujdate and Afourare) to investigate their seed oil, phenolic and flavonoid contents, the balance between omega‐6, omega‐9, and omega‐3 fatty acids, iodine value, and susceptibility to oxidation. Genotype and environment main effects as well as their interaction had a significant effect on the majority of the parameters studied. The mutant “US2‐1” stands out with a distinctive fatty acid profile, featuring the highest saturated fatty acids, ω‐6/ω‐9 ratio, and omega‐3 content, in addition to its high total flavonoid content and remarkable oxidative stability. “ML2‐10,” “US1‐2,” “US2‐6,” and “US2‐7” exhibit high oil content, coupled with substantial levels of phenols and flavonoids, rendering them excellent candidates for culinary and industrial applications where oxidative stability is important. “ML2‐68,” “ML2‐72,” “US06,” “US1‐DL,” and “US1‐3” offer versatile options with their moderate oil content and fatty acid profiles that resist oxidation. This research highlights the potential for breeding sesame mutants tailored to specific applications, contributing to both enhanced oil production and improved nutritional value. The insights gained here pave the way for the development of sesame varieties with superior nutritional and functional attributes, thereby promoting the sustainable production and consumption of sesame oil.

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The influence of substrate systems on the enantioselective and lipolytic activity of immobilized Amano PS from Burkholderia cepacia lipase (APS-BCL)

Cited by 9 publications

References 104 publications

The High ‘Lipolytic Jump’ of Immobilized Amano A Lipase from Aspergillus niger in Developed ‘ESS Catalytic Triangles’ Containing Natural Origin Substrates

The High ‘Lipolytic Jump’ of Immobilized Amano A Lipase from Aspergillus niger in Developed ‘ESS Catalytic Triangles’ Containing Natural Origin Substrates

Biocatalysts Synthesized with Lipase from Pseudomonas cepacia on Glycol-Modified ZIF-8: Characterization and Utilization in the Synthesis of Green Biodiesel

Exploring fatty acid composition, bioactive compounds, and antioxidant properties in oils of newly developed sesame mutant lines in Morocco

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