The influence of serotonin and p-chlorophenylalanine on locomotor activity of Drosophila melanogaster

Kamyshev, N. G.; Smirnova, G. P.; Savvateeva, Elena V.; Medvedeva, A. V.; Ponomarenko, V. V.

doi:10.1016/0091-3057(83)90005-9

Cited by 24 publications

(16 citation statements)

References 14 publications

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“…In adult Drosophila and other insects, 5HT has been reported to modulate circadian rhythms, reproduction, feeding, heart rate and locomotion [90] [91] [92] [93] [94], besides light-dependent locomotion, olfaction and feeding [42], [59], [60]. However, flies having diminished neuronal 5HT are still viable and fertile.…”

Section: Discussionmentioning

confidence: 99%

The Serotonergic Central Nervous System of the Drosophila Larva: Anatomy and Behavioral Function

et al. 2012

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The Drosophila larva has turned into a particularly simple model system for studying the neuronal basis of innate behaviors and higher brain functions. Neuronal networks involved in olfaction, gustation, vision and learning and memory have been described during the last decade, often up to the single-cell level. Thus, most of these sensory networks are substantially defined, from the sensory level up to third-order neurons. This is especially true for the olfactory system of the larva. Given the wealth of genetic tools in Drosophila it is now possible to address the question how modulatory systems interfere with sensory systems and affect learning and memory. Here we focus on the serotonergic system that was shown to be involved in mammalian and insect sensory perception as well as learning and memory. Larval studies suggested that the serotonergic system is involved in the modulation of olfaction, feeding, vision and heart rate regulation. In a dual anatomical and behavioral approach we describe the basic anatomy of the larval serotonergic system, down to the single-cell level. In parallel, by expressing apoptosis-inducing genes during embryonic and larval development, we ablate most of the serotonergic neurons within the larval central nervous system. When testing these animals for naïve odor, sugar, salt and light perception, no profound phenotype was detectable; even appetitive and aversive learning was normal. Our results provide the first comprehensive description of the neuronal network of the larval serotonergic system. Moreover, they suggest that serotonin per se is not necessary for any of the behaviors tested. However, our data do not exclude that this system may modulate or fine-tune a wide set of behaviors, similar to its reported function in other insect species or in mammals. Based on our observations and the availability of a wide variety of genetic tools, this issue can now be addressed.

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Section: Discussionmentioning

confidence: 99%

The Serotonergic Central Nervous System of the Drosophila Larva: Anatomy and Behavioral Function

et al. 2012

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“…The development and distribution of 5‐HT‐immunoreactive neurons in the CNS have been established (Valles & White, 1988). 5‐HT has a role in many physiological processes with regard to regulating locomotion and cardiac output (Kamyshev et al. , 1983; Johnson et al.…”

Section: Introductionmentioning

confidence: 99%

Influence of PCPA and MDMA (ecstasy) on physiology, development and behavior in Drosophila melanogaster

Dasari

Viele

Turner

et al. 2007

Eur J of Neuroscience

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The effects of para-chlorophenylalanine (PCPA) and 3,4 methylenedioxy-methamphetamine (MDMA, 'ecstasy') were investigated in relation to development, behavior and physiology in larval Drosophila. PCPA blocks the synthesis of serotonin (5-HT) and MDMA is known to deplete 5-HT in mammalian neurons; thus these studies were conducted primarily to target the serotonergic system. Treatment with PCPA and MDMA delayed time to pupation and eclosion. The developmental rate was investigated with a survival analysis statistical approach that is unique for Drosophila studies. Locomotion and eating were reduced in animals exposed to MDMA or PCPA. Sensitivity to exogenously applied 5-HT on an evoked sensory-central nervous system (CNS)-motor circuit showed that the CNS is sensitive to 5-HT but that when depleted of 5-HT by PCPA a decreased sensitivity occurred. A diet with MDMA produced an enhanced response to exogenous 5-HT on the central circuit. Larvae eating MDMA from the first to third instar did not show a reduction in 5-HT within the CNS; however, eating PCPA reduced 5-HT as well as dopamine content as measured by high performance liquid chromatography from larval brains. As the heart serves as a good bioindex of 5-HT exposure, it was used in larvae fed PCPA and MDMA but no significant effects occurred with exogenous 5-HT. In summary, the action of these pharmacological compounds altered larval behaviors and development. PCPA treatment changed the sensitivity in the CNS to 5-HT, suggesting that 5-HT receptor regulation is modulated by neural activity of the serotonergic neurons. The actions of acute MDMA exposure suggest a 5-HT agonist action or possible dumping of 5-HT from neurons.

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“…On the other hand, little or no locomotor activity is observed in adult animals expressing a mutation for a biosynthetic enzyme common to two other neuroactive amines, octopamine (Oct) and its precursor tyramine (Tyr), which suggest these BAs also regulate locomotion [4]. Moreover, injection of female flies with a different BA, serotonin (5HT), induces a dose-dependent increase in locomotor activity [5]. These behavioral data are consistent with genetic information obtained by Jordan et al, 2006 [6] who carried out a quantitative trait loci analysis to identify genes associated to locomotor behavior in adult flies, including tyr1 , a gene affecting catecholamine biosynthesis, Catsup , a negative regulator of TH activity, and Dopa decarboxylase ( Ddc ), an enzyme associated to DA and serotonin (5HT) biosynthesis [6].…”

Section: Introductionmentioning

confidence: 99%

Serotonin Receptors Expressed in Drosophila Mushroom Bodies Differentially Modulate Larval Locomotion

et al. 2014

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Drosophila melanogaster has been successfully used as a simple model to study the cellular and molecular mechanisms underlying behaviors, including the generation of motor programs. Thus, it has been shown that, as in vertebrates, CNS biogenic amines (BA) including serotonin (5HT) participate in motor control in Drosophila. Several evidence show that BA systems innervate an important association area in the insect brain previously associated to the planning and/or execution of motor programs, the Mushroom Bodies (MB). The main objective of this work is to evaluate the contribution of 5HT and its receptors expressed in MB to motor behavior in fly larva. Locomotion was evaluated using an automated tracking system, in Drosophila larvae (3rd-instar) exposed to drugs that affect the serotonergic neuronal transmission: alpha-methyl-L-dopa, MDMA and fluoxetine. In addition, animals expressing mutations in the 5HT biosynthetic enzymes or in any of the previously identified receptors for this amine (5HT1AR, 5HT1BR, 5HT2R and 5HT7R) were evaluated in their locomotion. Finally, RNAi directed to the Drosophila 5HT receptor transcripts were expressed in MB and the effect of this manipulation on motor behavior was assessed. Data obtained in the mutants and in animals exposed to the serotonergic drugs, suggest that 5HT systems are important regulators of motor programs in fly larvae. Studies carried out in animals pan-neuronally expressing the RNAi for each of the serotonergic receptors, support this idea and further suggest that CNS 5HT pathways play a role in motor control. Moreover, animals expressing an RNAi for 5HT1BR, 5HT2R and 5HT7R in MB show increased motor behavior, while no effect is observed when the RNAi for 5HT1AR is expressed in this region. Thus, our data suggest that CNS 5HT systems are involved in motor control, and that 5HT receptors expressed in MB differentially modulate motor programs in fly larvae.

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The influence of serotonin and p-chlorophenylalanine on locomotor activity of Drosophila melanogaster

Cited by 24 publications

References 14 publications

The Serotonergic Central Nervous System of the Drosophila Larva: Anatomy and Behavioral Function

The Serotonergic Central Nervous System of the Drosophila Larva: Anatomy and Behavioral Function

Influence of PCPA and MDMA (ecstasy) on physiology, development and behavior in Drosophila melanogaster

Serotonin Receptors Expressed in Drosophila Mushroom Bodies Differentially Modulate Larval Locomotion

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