2015
DOI: 10.1111/1556-4029.12893
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The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces

Abstract: Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real-time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2-indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolat… Show more

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Cited by 15 publications
(9 citation statements)
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References 15 publications
(15 reference statements)
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“…This indicates that the treatment of samples did not result in any inhibition for the PCR. These results reflect other studies [49][50][51][52][53][54] which reported that reagents commonly used to develop latent marks do not adversely affect subsequent recovery of DNA.…”
Section: Accepted Manuscriptsupporting
confidence: 89%
See 1 more Smart Citation
“…This indicates that the treatment of samples did not result in any inhibition for the PCR. These results reflect other studies [49][50][51][52][53][54] which reported that reagents commonly used to develop latent marks do not adversely affect subsequent recovery of DNA.…”
Section: Accepted Manuscriptsupporting
confidence: 89%
“…With fingermarks enhancement, several studies [49][50][51][52][53][54] have shown that most of the reagents commonly used to develop latent marks, such as aluminium powder, black magnetic powder, fluorescent powders, wet powder suspension and 1,2-indanedione do not affect subsequent recovery of DNA. Other specific chemicals such as cyanoacrylate and ninhydrin may have a negative impact on the process as they can either degrade or sequester the DNA or inhibit the PCR reaction [55,56].…”
Section: Accepted Manuscriptmentioning
confidence: 99%
“…The identification method is pertinent for the reliable identification of a person and is widely considered in forensics investigations and civil applications [2]. Fingerprints are the skin impressions left on any surface due to the friction ridge of finger.…”
Section: Introductionmentioning
confidence: 99%
“…Discussion: The good performance of sticky notes in the ninhydrin test compared to other techniques may be due to extraction removing sampled Ruhemann's purple, a suspected amplification inhibitor [5]. Reduced profile completeness across all techniques in the ninhydrin test may be due to physical removal of DNA during the ninhydrin wash step: the similar wash step of coumassie blue staining causes such reduction [6]. Reduced profile completeness in the confirmatory study may be due to considerably less starting material for analysis, with only halfprints used.…”
mentioning
confidence: 99%