The Influence of Sampling Time to Serum Glial Fibrillary Acidic Protein (Gfap) Levels in Acute Stroke

Abstract

Background: Serum Glial Fibrillary Acidic Protein (GFAP) is a great potential for biomarker that is widely studied as a diagnostic biomarker of acute stroke. Sampling within 6 hours after onset is the best time window, but in Indonesia, stroke patients often arrive late more than 6 hours. Objective: To identify the difference in time of blood sampling with serum GFAP levels within 24 hours onset of ischemic stroke (IS) patients and intracerebral hemorrhage (ICH) strokes. Methods: Cross-sectional analysis with purposive sampling, sampling in IS and ICH strokes that arrive at the ER within 24-hour on-site. The serum GFAP examination was performed with ELISA. Results: In this study, 41 acute stroke patients with 24-hour onset of each stroke were grouped into group 1 (<6 hours), group 2 (6-12 hours) and group 3 (12-24 hours). One Way ANOVA and Tukey's analysis showed no significant difference in GFAP levels among the three groups in both IS and ICH. Conclusion: There was no significant difference in GFAP levels in samples <6 hours, 6-12 hours, and 12-24 hours in ischemic strokes and ICH strokes.

Evaluation of the Canadian Ct Head Rule for Analyzing Diagnostic Accuracy in Patients 4 With Minor Head Trauma

“…It has been known that in acute ischemic stroke, neuronal damage and necrosis progresses slowly. 8 CT might not always be effective in hyperacute stage of stroke but it is still a very useful tool to detect ischemia of brain tissue. In addition, head CT is a very important diagnostic tool that aids in the rapid diagnosis of neurotraumas and is relatively inexpensive in comparison with magnetic resonance imaging.…”

“…5,6 Non-contrast head computed tomography (CT) has become the standard imaging method to detect brain injuries in patients with head traumas. 7 CT is a fast and highly sensitive technique for the detection of acute brain hemorrhages and skull fractures 1,8 and costs less than magnetic resonance imaging. 1 Conversely, the cost and ionizing radiation exposure associated with head CT are the two most important disadvantages.…”

The effect of addition protein, phosphatidylcholine, phosphatidylserine, and inulin on GFAP levels of acute ischemic stroke patients at Dr. Kariadi Hospital, Semarang

“…7 This is different from the study conducted by Neila et al, which aims to determine the difference between blood sampling time and serum GFAP levels within 24 hours of onset in patients with ischemic stroke and hemorrhagic stroke, which shows that there was no significant difference in GFAP levels in samples <6 hours, 6-12 hours, and 12-24 hours in ischemic strokes and ICH strokes. 8 Factors that can influence GFAP include some cellular processes. In general, GFAP has an important role in the regulation between astrocytes and neurons.…”