The influence of RGD addition on the gene transfer characteristics of disulfide-containing polyethyleneimine/DNA complexes

Sun, Yunxia; Zhang, Xuan; Meng, Qingfei; Zhang, Xian‐Zheng; Cheng, Si‐Xue; Zhuo, Ren‐Xi

doi:10.1016/j.biomaterials.2008.07.045

Cited by 122 publications

(103 citation statements)

References 25 publications

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“…The strategy of preparing HMW polycations from low-molecular weight (LMW) oligocations via biodegradable linkages has been proven to reduce toxicity while retaining the gene transfer ability of the polyplexes. Up to now, several examples had been reported (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Hydrolysable PEIs were developed by cross-linking oligoethylenimine 800 Da (OEI) with ester bond bearing monomers or polyesters (6)(7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

“…Biocleavable PEIs were prepared by thiolation of LMW PEI (13,14) or by cross-linking of OEI with disulfide bond containing linkers, including dimethyl 3, 3′-dithiobis(propionimidate) and dithiobis (succinimidyl propionate) (6,15,16). Bioreducible copolymers from N,N′-cystamine bisacrylamide (CBA) linker and different primary amines or oligoamines were explored too (17)(18)(19)(20)(21). The polyplexes from these hydrolysable or bioreducible polycations were designed to keep stable in extracellular environment while dissociate efficiently upon intracellular delivery when exposed to reducing intracellular condition (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

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Influence of the Molecular Weight of Bioreducible Oligoethylenimine Conjugates on the Polyplex Transfection Properties

Ruß

Wagner

2009

AAPS J

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Abstract. The purpose of the present study was to investigate the influence of molecular weights on the chemical, biophysical, and biological properties of bioreducible oligoethylenimine conjugates. The cationic conjugates were synthesized by polyaddition between branched oligoethylenimine 800 Da (OEI) and the disulfide bond containing N,N′-cystamine bisacrylamide (CBA) linker. A correlation between the copolymer molecular weights and the polyplex transfection properties was found. The OEI-CBA copolymers differing in molecular weights (from 8.6 to 37 kDa) showed good plasmid DNA binding ability resulting in compact 90-to 150-nm-sized polyplexes. Colloidal stability of the polyplexes was lost in reductive environment. A low concentration of dithiothreitol of 5 µM was sufficient to render polyplexes unstable in size. Reducing conditions at physiological salt concentration triggered polyplex dissociation. The bioreducible polymers displayed much lower cytotoxicity (IC 50 ∼100 μg/mL in cell culture) than branched polyethylenimine 25 kDa (BPEI) and linear polyethylenimine 22 kDa (LPEI). Reporter gene transfection experiments were done with CHO-K1 and B16-F10 cells. The largest (37 kDa) copolymer HC-6-8 demonstrated highest transfection levels among all the bioreducible copolymers, which was comparable with LPEI and much more effective than BPEI.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of the Molecular Weight of Bioreducible Oligoethylenimine Conjugates on the Polyplex Transfection Properties

Ruß

Wagner

2009

AAPS J

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“…55 As a result, a huge amount of research has been done to develop bioreducible polymeric vectors containing disulfide linkages. 54,56 Sun et al 57 introduced a tripeptide sequence RGD onto a reducible polymer through physical adsorption in order to prevent possible reduction or inactivation of the biological activities of RGD peptide arising from a covalent attachment (Figure 1d). The RGD peptide was added to disulfide-containing PEI (SS-PEI)/DNA binary complexes, and the influence of RGD addition on the particle size, zeta potential, morphology and transfection efficiency was investigated.…”

Section: S-s-pei/rgdmentioning

confidence: 99%

A review of RGD-functionalized nonviral gene delivery vectors for cancer therapy

et al. 2012

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The development of effective treatments that enable many patients suffering from cancer to be successfully cured is highly demanded. Angiogenesis, which is a process for the formation of new capillary blood vessels, has a crucial role in solid tumor progression and the development of metastasis. Antiangiogenic therapy designed to prevent tumor angiogenesis, thereby arresting the growth or spread of tumors, has emerged as a non-invasive and safe option for cancer treatment. Due to the fact that integrin receptors are overexpressed on the surface of angiogenic endothelial cells, various strategies have been made to develop targeted delivery systems for cancer gene therapy utilizing integrin-targeting peptides with an exposed arginine-glycine-aspartate (RGD) sequence. The aim of this review is to summarize the progress and prospect of RGD-functionalized nonviral vectors toward targeted delivery of genetic materials in order to achieve an efficient therapeutic outcome for cancer gene therapy, including antiangiogenic therapy.

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“…20 These contrasting effects associated with the use of PEG in oligonucleotide delivery are also referred to as the "PEG dilemma". 21 Guanidine is one of the strongest organic bases with some biological activities. Nitrogen and hydrogen atoms in the guanidino (Gua) group have a strong affinity to carbonic acid esters, phosphate esters, and peptides, leading to the formation of hydrogen bonds with some geometric configurations.…”

Section: Introductionmentioning

confidence: 99%

Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery

Zhang

Xing

et al. 2016

IJN

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The influence of RGD addition on the gene transfer characteristics of disulfide-containing polyethyleneimine/DNA complexes

Cited by 122 publications

References 25 publications

Influence of the Molecular Weight of Bioreducible Oligoethylenimine Conjugates on the Polyplex Transfection Properties

Influence of the Molecular Weight of Bioreducible Oligoethylenimine Conjugates on the Polyplex Transfection Properties

A review of RGD-functionalized nonviral gene delivery vectors for cancer therapy

Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery

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