The Influence of Radiation Quality on Radiation-induced Hemolysis and Hemoglobin Oxidation of Human Erythrocytes

Puchała, Mieczysław; Szweda‐Lewandowska, Zofia; Kiefer, Juegen

doi:10.1269/jrr.45.275

Cited by 37 publications

(19 citation statements)

References 20 publications

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“…The present experimental results, along with published data [29], lead to the conclusion that H 2 O 2 at the concentration used here appears not to play any significant role in stimulating peroxidation processes in erythrocyte membranes, because of endogenous catalase activity. On the other hand, the superoxide anion, which, along with H 2 O 2 , is formed in aqueous medium as a result of the radiolysis of water, also cannot have a strong damaging influence on erythrocyte membranes [30].…”

Section: Resultssupporting

confidence: 80%

Studies of the antioxidant and antihemolytic activity of quinoline derivatives in a model of oxidative damage to erythrocyte membranes

Malakyan¹,

Badzhinyan²,

Vardevanyan³

et al. 2009

Pharm Chem J

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New benzo-substituted 4-(aminophenylamino)-2-methylquinolines, 3-substituted 4-hydroxy(chloro and mercapto)quinolines, and thienoquinolines were synthesized. A model system based on oxidative damage to erythrocyte membranes induced by the combined actions of hydrogen peroxide and ionizing radiation was used to study the antihemolytic effects of these compounds as a means of assessing their antiradical/antioxidant properties. In the absence of x-rays, substances of the 3-substituted 4-hydroxy(chloro and mercapto)quinoline and thienoquinoline series were found to have marked antihemolytic effects, associated with significant influences on the structural stability of erythrocytes. However, with irradiation, the membrane-protecting effects were more marked with benzo-substituted 4-(aminophenylamino)-2-methylquinoline derivatives.

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Section: Resultssupporting

confidence: 80%

Studies of the antioxidant and antihemolytic activity of quinoline derivatives in a model of oxidative damage to erythrocyte membranes

Malakyan¹,

Badzhinyan²,

Vardevanyan³

et al. 2009

Pharm Chem J

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“…Reticulocytes and red blood cells (RBC) are highly radioresistant due to their lack of DNA and apoptotic machinery [4]. However, sublethal total body irradiation (TBI) results in the rapid decrease of total circulating reticulocytes [5–8] and an increase in reticulocytes containing DNA fragments or lagging chromosomes, so called micronucleated reticulocytes (MN-RET) [9–13].…”

Section: Introductionmentioning

confidence: 99%

Sublethal radiation injury uncovers a functional transition during erythroid maturation

Peslak

Wenger

Bemis

et al. 2011

Experimental Hematology

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Objective Clastogenic injury of the erythroid lineage results in anemia, reticulocytopenia, and transient appearance of micronucleated reticulocytes (MN-RET). However, the MN-RET dose-response in murine models is only linear to 2 Gy total body irradiation (TBI) and paradoxically decreases at higher exposures, suggesting complex radiation effects on erythroid intermediates. To better understand this phenomenon, we investigated the kinetics and apoptotic response of the erythron to sublethal radiation injury. Materials and Methods We analyzed the response to 1 and 4 Gy TBI of erythroid progenitors and precursors using colony assays and imaging flow cytometry (IFC), respectively. We also investigated cell cycling and apoptotic gene expression of the steady-state erythron. Results Following 1 Gy TBI, erythroid progenitors and precursors were partially depleted. In contrast, essentially all bone marrow erythroid progenitors and precursors were lost within two days following 4 Gy irradiation. IFC analysis revealed preferential loss of phenotypic erythroid colony-forming units (CFU-E) and proerythroblasts immediately following sublethal irradiation. Furthermore, these populations underwent radiation-induced apoptosis, without changes in steady-state cellular proliferation, at much higher frequencies than later-stage erythroid precursors. Primary erythroid precursor maturation is associated with marked Bcl-xL upregulation and Bax and Bid down-regulation. Conclusions MN-RET loss following higher sublethal radiation exposures results from rapid depletion of erythroid progenitors and precursors. This injury reveals that CFU-E and proerythroblasts constitute a particularly proapoptotic compartment within the erythron. We conclude that the functional transition of primary proerythroblasts to later-stage erythroid precursors is characterized by a shift from a pro-apoptotic to an anti-apoptotic phenotype.

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“…In the present study it was observed that the RBC counts, Hb contents, and HCT of mice in all groups A B exposed to a sub-lethal dose (4 Gy) of gamma-irradiation at day 1 post-irradiation did not show any decline compared with the normal value, which mainly because erythrocytes can live for 120 days and the mature RBCs are very radioresistant owing to their lack of DNA and apoptotic machinery (Puchała et al, 2004). The RBC counts, Hb contents, and HCT of mice pretreated with KA (300 mg/kg body weight) reverted to normal at day 14, but mice irradiated alone did not, which indicates KA provides protection to RBCs and hemoglobin contents, and protects against anaemia induced by ionizing radiation.…”

Section: Discussionmentioning

confidence: 45%

Kojic Acid Protects C57BL/6 Mice from Gamma-irradiation Induced Damage

Wang¹,

Liu²,

Di³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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The radioprotective effects of a single administration of kojic acid (KA) against ionizing radiation were evaluated via assessment of 30-day survival and alterations of peripheral blood parameters of adult C57BL/6 male mice. The 30-day survival rate of mice pretreated with KA (75 or 300 mg/kg body weight, KA75 or KA300) subcutaneously 27 h prior to a lethal dose (8 Gy, 153.52 cGy/min) of gamma irradiation was higher than that of mice irradiated alone (40% or 60% vs 0%). It was observed that the white blood cell (WBC) count/the red blood cell (RBC) count, haemoglobin content, haematocrit and platelet count of mice with or without KA pretreatment as exposed to a sub-lethal dose (4 Gy, 148.14 cGy/min) of gamma irradiation decreased maximally at day 4/day 8 post-irradiation. Although the initial WBC values were low in KA300 or WR-2721 (amifostine) groups, they significantly recovered to normal at day 19, whereas in the control group they did not. The results from the cytotoxicity and cell viability assays demonstrated that KA could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity. In summary, KA provides marked radioprotective effects both in vivo and in vitro.

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The Influence of Radiation Quality on Radiation-induced Hemolysis and Hemoglobin Oxidation of Human Erythrocytes

Cited by 37 publications

References 20 publications

Studies of the antioxidant and antihemolytic activity of quinoline derivatives in a model of oxidative damage to erythrocyte membranes

Studies of the antioxidant and antihemolytic activity of quinoline derivatives in a model of oxidative damage to erythrocyte membranes

Sublethal radiation injury uncovers a functional transition during erythroid maturation

Kojic Acid Protects C57BL/6 Mice from Gamma-irradiation Induced Damage

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