The influence of post-infarct heart failure and high fat diet on the expression of apelin APJ and vasopressin V1a and V1b receptors

Czarzasta, Katarzyna; Wojno, Olena; Żera, Tymoteusz; Puchalska, Liana; Dobruch, Jakub; Cudnoch-Jędrzejewska, Agnieszka

doi:10.1016/j.npep.2019.101975

Cited by 13 publications

(16 citation statements)

References 69 publications

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“…Moreover, the expression of Apelin and Sirt3 was downregulated in HGtreated cardiomyocytes and DCM. Mounting evidence suggests that Apelin acts as an important adipokine against diabetes (Castan-Laurell et al, 2011;Czarzasta et al, 2019). Hence, as expected, the overexpression of Apelin reversed the mitochondrial dysfunction ( Figures 3A-F).…”

Section: Discussionsupporting

confidence: 75%

Icariin Ameliorates Diabetic Cardiomyopathy Through Apelin/Sirt3 Signalling to Improve Mitochondrial Dysfunction

Lin

Huang

et al. 2020

Front. Pharmacol.

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Myocardial contractile dysfunction in diabetic cardiomyocytes is a significant promoter of heart failure. Herein, we investigated the effect of icariin, a flavonoid monomer isolated from Epimedium, on diabetic cardiomyopathy (DCM) and explored the mechanisms underlying its unique pharmacological cardioprotective functions. High glucose (HG) conditions were simulated in vitro using cardiomyocytes isolated from neonatal C57 mice, while DCM was stimulated in vivo in db/db mice. Mice and cardiomyocytes were treated with icariin, with or without overexpression or silencing of Apelin and Sirt3 via transfection with adenoviral vectors (Ad-RNA) and specific small hairpin RNAs (Ad-sh-RNA), respectively. Icariin markedly improved mitochondrial function both in vivo and in vitro, as evidenced by an increased level of mitochondrial-related proteins via western blot analysis (PGC-1a, Mfn2, and Cyt-b) and an increased mitochondrial membrane potential, as observed via JC-1 staining. Further, icariin treatment decreased cardiac fibrogenesis (Masson staining), and inhibited apoptosis (TUNEL staining). Together, these changes improved cardiac function, according to multiple transthoracic echocardiography parameters, including LVEF, LVSF, LVESD, and LVEDD. Moreover, icariin significantly activated Apelin and Sirt3, which were inhibited by HG and DCM. Importantly, when Ad-sh-Apelin and Ad-sh-Sirt3 were transfected in cardiomyocytes or injected into the heart of db/db mice, the cardioprotective effects of icariin were abolished and mitochondrial homeostasis was disrupted. Further, it was postulated that since Ad-Apelin

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Section: Discussionsupporting

confidence: 75%

Icariin Ameliorates Diabetic Cardiomyopathy Through Apelin/Sirt3 Signalling to Improve Mitochondrial Dysfunction

Lin

Huang

et al. 2020

Front. Pharmacol.

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“…Fragments of the medulla oblongata homogenized and the RNA were obtained as described earlier [ 14 ]. RT-PCR analysis was performed with the TaqMan ® RNA-to-Ct™ 1-Step Kit and the starter for the following gene: rat OXR1 (Hcrtr1, Rn00565032_m1; Life Technologies); rat V1aR (Avpr1a, Rn00583910_m1; Life Technologies) tagged with a FAM pigment, a starter for the rat GADPH (Rn01775763_g1; Life Technologies) tagged with a VIC pigment.…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR analysis was performed with the TaqMan ® RNA-to-Ct™ 1-Step Kit and the starter for the following gene: rat OXR1 (Hcrtr1, Rn00565032_m1; Life Technologies); rat V1aR (Avpr1a, Rn00583910_m1; Life Technologies) tagged with a FAM pigment, a starter for the rat GADPH (Rn01775763_g1; Life Technologies) tagged with a VIC pigment. The RT-PCR analysis was performed as described previously [ 12 , 14 ], using a ViiA™ 7 Real-Time PCR System thermocycler (Life Technologies). The comparative gene expressions are shown as ΔCt in arbitrary units.…”

Section: Methodsmentioning

confidence: 99%

“…Fragments of the medulla oblongata were homogenized and western blot analysis was performed according to the protocol described earlier [ 14 ]. We used the following antibodies: a primary goat polyclonal antibody against OX1R (ab224368; Abcam), a primary rabbit polyclonal antibody against V1aR (sc-30025; Santa Cruz Biotechnology), a primary rabbit polyclonal antibody anti-β Actin (ab8227; Abcam), and a secondary antibody: mouse anti-rabbit conjugated to Horseradish Peroxidase (HRP) (sc-2357; Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

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Interaction of Orexin A and Vasopressin in the Brain Plays a Role in Blood Pressure Regulation in WKY and SHR Rats

et al. 2020

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Background Orexin A (OXA) and vasopressin (AVP) exert a central hypertensive effect due to an increase in sympathetic nerve activity. To date, little is known about the interaction of these 2 neuropeptides in the central regulation of blood pressure. The present study compared the consequences of infusion into the left cerebral ventricle (ICV) of OXA on mean arterial blood pressure (MABP) in normotensive (WKY) and spontaneously hypertensive (SHR) rats, and explored whether the central pressor action of OXA in these 2 strains depends on activation of brain AVP V1a receptors (V1aR). Material/Methods Ten groups of experiments were performed on 12-week-old WKY and SHR rats implanted with ICV cannulas for infusion of OXA (3 nmol) and V1aR antagonist (V1aRANT, 500 ng), administered separately and together. Levels of V1aR and OXR in the medulla oblongata of WKY and SHR rats were compared in separate series. Results We found that: 1) OXA significantly increased MABP only in WKY rats, 2) V1aRANT prevented an increase in MABP induced by OXA in WKY rats and decreased MABP in SHR rats, 3) OXA abolished the hypotensive action of V1aRANT in SHR rats, and 4) SHR rats had significantly higher levels of OX1R and V1aR proteins and OX1R mRNA in the brain medulla. Conclusions The present study shows that OXA and AVP can interact in the brain to affect blood pressure regulation, and that this interaction differs in normotension and hypertension.

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“…It has been demonstrated that a HFD may modify both the central apelinergic and vasopressinergic systems 17‐19 . To date, it has not been explained how a high‐fat diet affects the central activity of the apelinergic and vasopressinergic systems.…”

Section: Introductionmentioning

confidence: 99%

Central interaction between the apelinergic and vasopressinergic systems in the regulation of the haemodynamic parameters in rats maintained on a high‐fat diet

Wojno

Czarzasta

Puchalska

et al. 2020

Clin Exp Pharma Physio

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A high‐fat diet can affect the central activity of the apelinergic and vasopressinergic systems, which can have a significant impact on cardiovascular regulation. The aim of the study was to investigate the role of the central interaction between apelin and vasopressin in the regulation of the cardiovascular system in Sprague Dawley rats maintained on a normal‐fat diet (NFD) or on a high‐fat diet (HFD). The animals were instrumented with a cannula implanted into the left cerebral ventricle for intracerebroventricular (ICV) infusions of saline (0.9% NaCl), apelin‐13 (APLN‐13), V1a receptor antagonist (V1aRANT) APJ receptor antagonist (F13A), vasopressin (AVP); and with a catheter placed within the femoral artery for mean arterial blood pressure and heart rate monitoring. Blood, the hypothalamus and the medulla oblongata were collected for biochemical analysis. The hypertensive effect of APLN‐13 was blocked by a prior ICV infusion of V1aRANT, only in the NFD rats. However, the hypertensive effect of AVP was blocked by the prior ICV infusion of F13A in both the NFD and HFD rats. A HFD caused an increase in the protein level of APJ and V1a receptors, both in the hypothalamus and the medulla oblongata. This study confirms the presence of an interaction between both peptides in the central regulation of the cardiovascular system in rats on a NFD or a HFD.

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The influence of post-infarct heart failure and high fat diet on the expression of apelin APJ and vasopressin V1a and V1b receptors

Cited by 13 publications

References 69 publications

Icariin Ameliorates Diabetic Cardiomyopathy Through Apelin/Sirt3 Signalling to Improve Mitochondrial Dysfunction

Icariin Ameliorates Diabetic Cardiomyopathy Through Apelin/Sirt3 Signalling to Improve Mitochondrial Dysfunction

Interaction of Orexin A and Vasopressin in the Brain Plays a Role in Blood Pressure Regulation in WKY and SHR Rats

Central interaction between the apelinergic and vasopressinergic systems in the regulation of the haemodynamic parameters in rats maintained on a high‐fat diet

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