The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers

Sanami, Mohammad; Sweeney, I; Shtein, Zvi; Meirovich, Sigal; Sorushanova, Anna; Mullen, Anne Maria; Miraftab, Mohsen; Shoseyov, Oded; O'Dowd, Colm; Pandit, Abhay; Zeugolis, Dimitrios I.

doi:10.1002/jbm.b.33445

Cited by 26 publications

(38 citation statements)

References 65 publications

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“…1B). However, as most self-assembled biomaterials [13], these membranes lack the mechanical properties required for many biomedical applications due to the nature of non-covalent interactions involved in the self-assembly of molecules, which limits their applicability. Indeed, hydrogen bonds have very low association strength in hydrogels due to competition of water for binding sites, hydrophobic interactions are highly limited by solubility of the hydrophobes in solution [14], and hydrophilic polymers tend to dissolve into the aqueous phase [15].…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…Different chemical, biological, and physical cross-linking strategies have been proposed to increase the mechanical properties of self-assembled materials [13,16]. The ideal cross-linker should be capable to covalently link molecular groups of these building-blocks without compromising their hierarchical order, or eliciting cytotoxic, or immune reactions [17].…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…Therefore, cross-linking strategies based on multifunctional poly (ethylene glycol) (PEG) systems and plant extracts have been proposed as alternative cross-linker candidates. Multifunctional PEG cross-linking systems are either linear or exhibit branched chains with terminal active ester groups that react with the primary amine groups of the building blocks [13,18]. Poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG), a multi-armed functional PEG molecule, has been reported as an effective cross-linker of hydrogels [18,19] and implantable fibres [13].…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…Multifunctional PEG cross-linking systems are either linear or exhibit branched chains with terminal active ester groups that react with the primary amine groups of the building blocks [13,18]. Poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG), a multi-armed functional PEG molecule, has been reported as an effective cross-linker of hydrogels [18,19] and implantable fibres [13]. The resulting scaffolds demonstrated improved stability and mechanical properties in physiological environments while exhibiting good biocompatibility [18,19].…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…Four exogenous cross-linkers frequently used in tissue engineering applications [13,[16][17][18]23,30,31] were used including glutaraldehyde (GTA) [16,17,32], genipin (GNP) [15,22,33], poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) [12,17,30,31], and succinimidyl carboxymethyl ester (SCM-PEG-SCM) [29,34] (Fig. 2).…”

Section: Crosslinking Strategiesmentioning

confidence: 99%

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Cross-linking of a biopolymer-peptide co-assembling system

et al. 2017

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The ability to guide molecular self-assembly at the nanoscale into complex macroscopic structures could enable the development of functional synthetic materials that exhibit properties of natural tissues such as hierarchy, adaptability, and self-healing. However, the stability and structural integrity of these kinds of materials remains a challenge for many practical applications. We have recently developed a dynamic biopolymer-peptide co-assembly system with the capacity to grow and undergo morphogenesis into complex shapes. Here we explored the potential of different synthetic (succinimidyl carboxymethyl ester [SCM-PEG-SCM], poly (ethylene glycol) ether tetrasuccinimidyl glutarate [4S-StarPEG] and glutaraldehyde) and natural (genipin) cross-linking agents to stabilize membranes made from these biopolymer-peptide co-assemblies. We investigated the cross-linking efficiency, resistance to enzymatic degradation, and mechanical properties of the different cross-linked membranes. We also compared their biocompatibility by assessing the metabolic activity and morphology of adipose-derived stem cells (ADSC) cultured on the different membranes. While all cross-linkers successfully stabilized the system under physiological conditions, membranes cross-linked with genipin exhibited better resistance in physiological environments, improved stability under enzymatic degradation, and a higher degree of in vitro cytocompatibility compared to the other cross-linking agents. The results demonstrated that genipin is an attractive candidate to provide functional structural stability to complex self-assembling structures for potential tissue engineering or in vitro model applications. Statement of SignificanceMolecular self-assembly is widely used for the fabrication of complex functional biomaterials to replace and/or repair any tissue or organ in the body. However, maintaining the stability and corresponding functionality of these kinds of materials in physiological conditions remains a challenge. Chemical cross-linking strategies (natural or synthetic) have been used in an effort to improve their structural integrity. Here we investigate key performance parameters of different cross-linking strategies for stabilising self-assembled materials with potential biomedical applications using a novel protein-peptide co-assembling membrane as proof-of-concept. From the different cross-linkers tested, the natural cross-linker genipin exhibited the best performance. This cross-linker successfully enhanced the mechanical properties of the system enabling the maintenance of the structure in physiological conditions without compromising its bioactivity and biocompatibility. Altogether, we provide a systematic characterization of cross-linking alternatives for self-assembling materials focused on biocompatibility and stability and demonstrate that genipin is a promising alternative for the cross-linking of such materials with a wide variety of potential applications such as in tissue engineering and drug delivery.

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Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: Crosslinking Strategiesmentioning

confidence: 99%

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Cross-linking of a biopolymer-peptide co-assembling system

et al. 2017

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Harnessing Hierarchical Nano‐ and Micro‐Fabrication Technologies for Musculoskeletal Tissue Engineering

Abbah

Delgado

Azeem

et al. 2015

Adv Healthcare Materials

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Cells within a tissue are able to perceive, interpret and respond to the biophysical, biomechanical, and biochemical properties of the 3D extracellular matrix environment in which they reside. Such stimuli regulate cell adhesion, metabolic state, proliferation, migration, fate and lineage commitment, and ultimately, tissue morphogenesis and function. Current scaffold fabrication strategies in musculoskeletal tissue engineering seek to mimic the sophistication and comprehensiveness of nature to develop hierarchically assembled 3D implantable devices of different geometric dimensions (nano- to macrometric scales) that will offer control over cellular functions and ultimately achieve functional regeneration. Herein, advances and shortfalls of bottom-up (self-assembly, freeze-drying, rapid prototype, electrospinning) and top-down (imprinting) scaffold fabrication approaches, specific to musculoskeletal tissue engineering, are discussed and critically assessed.

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The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development

et al. 2018

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Collagen is the oldest and most abundant extracellular matrix protein that has found many applications in food, cosmetic, pharmaceutical, and biomedical industries. First, an overview of the family of collagens and their respective structures, conformation, and biosynthesis is provided. The advances and shortfalls of various collagen preparations (e.g., mammalian/marine extracted collagen, cell‐produced collagens, recombinant collagens, and collagen‐like peptides) and crosslinking technologies (e.g., chemical, physical, and biological) are then critically discussed. Subsequently, an array of structural, thermal, mechanical, biochemical, and biological assays is examined, which are developed to analyze and characterize collagenous structures. Lastly, a comprehensive review is provided on how advances in engineering, chemistry, and biology have enabled the development of bioactive, 3D structures (e.g., tissue grafts, biomaterials, cell‐assembled tissue equivalents) that closely imitate native supramolecular assemblies and have the capacity to deliver in a localized and sustained manner viable cell populations and/or bioactive/therapeutic molecules. Clearly, collagens have a long history in both evolution and biotechnology and continue to offer both challenges and exciting opportunities in regenerative medicine as nature's biomaterial of choice.

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The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers

Cited by 26 publications

References 65 publications

Cross-linking of a biopolymer-peptide co-assembling system

Cross-linking of a biopolymer-peptide co-assembling system

Harnessing Hierarchical Nano‐ and Micro‐Fabrication Technologies for Musculoskeletal Tissue Engineering

The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development

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