The Influence of Plant Growth Regulators on Callus Induction in Mature Rice Embryos (Oryza Sativa L) Amber Variety

Abstract

Several nations across the globe depend profoundly on rice as the main food source. Several research works have been undertaken to improve rice yield and breeding, specifically concerning plant tissue culture. This paper aims to contrast the impact of several growth regulator levels on rice callus induction based on mature embryo explants. Over a four-week duration, mature rice plant embryos (Amber variant) were planted to produce calluses using the Skoog (MS) and Murashige supplements at different concentration levels, i.e., 2.0, 2.5, and 3.0 mg/L of 2,4-D and 0.2 and 0.3 mg/L of BA. The outcomes indicated that 3 mg/L 2,4-D and 0.2 mg/L BA levels led to optimal induction and fresh weight concerning callus. Further studies are required to enhance the media environment for potent in vitro callus induction to augment biotechnology endeavours.