The influence of Piriformospora indica on the resistance of two rhododendron cultivars to Phytophthora cinnamomi and P. plurivora

Trzewik, A.; Maciorowski, Robert; Klocke, Evelyn; Orlikowska, T.

doi:10.1016/j.biocontrol.2019.104121

Cited by 22 publications

(22 citation statements)

References 40 publications

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“…It has also been shown to play another and even more important role in stimulating the resistance of Rhododendron to pathogens of the Phytophthora spp. (Trzewik et al 2020). Nevertheless, to develop a functional nursery technology enabling use of S. indica, further experiments aimed at the optimal fertilisation should be conducted.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of ex vitro growth of Rhododendron hybrids 'Nova Zembla' and 'Alfred' by inoculation of roots with Serendipita indica

Trzewik¹,

Orlikowska²,

Kowalczyk³

et al. 2020

Hortic. Sci.

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Experiments were carried out to study the consequences of inoculating Rhododendron ‘Nova Zembla’ and ‘Alfred’ microcuttings and young plants with Serendipita indica (formerly Piriformospora indica), an endophytic fungus. The inoculation at the in vitro rooting stage decreased the in vitro root and shoot quality, but after planting microplants in a greenhouse in a non-fertilised peat substrate, the plants grew 33% taller, with 10% more leaves and with more abundant roots in comparison with the non-inoculated microplants. The young plants rooted in vitro, acclimated in a greenhouse in a peat substrate and inoculated with S. indica at the time of the transplantation to the pots displayed, after 15 months, an increase in the shoot lengths by 35% and 13%, depending on the cultivar, in the leaf number by 47% and in the chlorophyll content by 31% when compared with the non-inoculated plants. The beneficial effect of S. indica on the young Rhododendron plants indicates the possibility of its practical use in the nursery production of this plant.

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Section: Discussionmentioning

confidence: 99%

Stimulation of ex vitro growth of Rhododendron hybrids 'Nova Zembla' and 'Alfred' by inoculation of roots with Serendipita indica

Trzewik¹,

Orlikowska²,

Kowalczyk³

et al. 2020

Hortic. Sci.

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“…Our previous work on reducing rhododendron Phytophthora root rot using S. indica showed 50% effectivity in protection against P. cinnamomi and P. plurivora [21]. One-year-old rhododendron plants infected with P. cinnamomi or P. plurivora died out within 6 weeks, while plants inoculated with S. indica 3 weeks before Phytophthora infection survived (50-60%) without any chemical treatment.…”

Section: Introductionmentioning

confidence: 95%

“…The fungus was activated to colonise plant roots by co-cultivation every 6 months with roots of Arabidopsis thaliana (Columbia-0 ecotype) on Petri dishes, according to the recommendations of Johnson [23]. The mycelium of S. indica used in the experiment was produced in the liquid KM medium, as described by Trzewik [21]. The peat substrate was inoculated with S. indica with a proportion of 2 g mycelium and 100 mL peat (2% w/v).…”

Section: Preparation Of S Indica Inoculum and Peat Substrate Inoculationmentioning

confidence: 99%

Protection of Highbush Blueberry Plants against Phytophthora cinnamomi Using Serendipita indica

2020

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A greenhouse experiment was carried out on the use of Serendipita indica, an endomycorrhizal-like fungus, to increase the resistance of three highbush blueberry cultivars Chandler, Darrow and Spartan to the pathogen Phytophthora cinnamomi. The cultivars differed in the acceptance of root colonisation by S. indica and in susceptibility to P. cinnamomi. The lowest percentage (%) of colonised roots was found in the cultivar Spartan. The frequency of symptomatic plants (stunted growth, dark spots on stems, small, yellowing, and dropping leaves) among plants inoculated only with P. cinnamomi was 59, 83, and 100%, for ‘Chandler’, ‘Darrow’, and ‘Spartan’, respectively. In the treatment where both pathogen and endophyte were inoculated, the frequency in ‘Darrow’ and ‘Spartan’ dropped to 28 and 90%, respectively. Of non-protected ‘Spartan’ plants, 66% died, whereas only 14% of those protected with S. indica died. Colonisation by S. indica reduced the number of dead plants of ‘Chandler’ from 3 to 1 among those protected. In ‘Darrow’ only one plant died of the non-protected and none of the protected ones. S. indica increased the growth of non-infected plants of ‘Darrow’ and ‘Spartan’ by 11%. An increase in the growth of plants inoculated with S. indica and infected with P. cinnamomi after 21 days went from 74% to 182% over the plants only infected with the pathogen.

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“…Supporting the use of biologicalcontrol for P. cinnamomi, Méndez-Bravo et al reported that two rhizobacteria, closely related to Bacillus acidiceler, were able to inhibit P. cinnamomi growth in vitro by 76%, suggesting that these bacteria could be used for biological control of oomycetes [77]. Lastly, Trzewik et al reported a practical possibility of biological protection against P. cinnamomi or Piriformospora indica, an endomycorrhizal-like fungus, in two cultivars of rhododendron plants ('Nova Zembla' and 'Alfred') [78].…”

Section: Biological Controlmentioning

confidence: 99%

Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches

2020

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The Phytophthora genus is composed, mainly, of plant pathogens. This genus belongs to the Oomycete class, also known as "pseudo-fungi", within the Chromista Kingdom. Phytophthora spp. is highlighted due to the significant plant diseases that they cause, which represents some of the most economically and cultural losses, such as European chestnut ink disease, which is caused by P. cinnamomi. Currently, there have been four genome assemblies placed at the National Center for Biotechnology Information (NCBI), although the progress to understand and elucidate the pathogenic process of P. cinnamomi by its genome is progressing slowly. In this review paper, we aim to report and discuss the recent findings related to P. cinnamomi and its genomic information. Our research is based on paper databases that reported probable functions to P. cinnamomi proteins using sequence alignments, bioinformatics, and biotechnology approaches. Some of these proteins studied have functions that are proposed to be involved in the asexual sporulation and zoosporogenesis leading to the host colonization and consequently associated with pathogenicity. Some remarkable genes and proteins discussed here are related to oospore development, inhibition of sporangium formation and cleavage, inhibition of flagellar assembly, blockage of cyst germination and hyphal extension, and biofilm proteins. Lastly, we report some biotechnological approaches using biological control, studies with genome sequencing of P. cinnamomi resistant plants, and gene silencing through RNA interference (iRNA).

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The influence of Piriformospora indica on the resistance of two rhododendron cultivars to Phytophthora cinnamomi and P. plurivora

Cited by 22 publications

References 40 publications

Stimulation of ex vitro growth of Rhododendron hybrids 'Nova Zembla' and 'Alfred' by inoculation of roots with Serendipita indica

Stimulation of ex vitro growth of Rhododendron hybrids 'Nova Zembla' and 'Alfred' by inoculation of roots with Serendipita indica

Protection of Highbush Blueberry Plants against Phytophthora cinnamomi Using Serendipita indica

Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches

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