The influence of phosphate deficiency on photosynthesis, respiration and adenine nucleotide pool in bean leaves

Mikulska, M.; Bomsel, Jean-Loup; Rychter, Anna M.

doi:10.1023/a:1006873932486

Cited by 34 publications

(17 citation statements)

References 31 publications

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“…Notably, like overexpression of intracellular HAD, PvPS2:1 also was found to up-regulate the transcripts of a PSR Pi transporter, leading to enhanced P content and superior P efficiency in Arabidopsis OE lines as compared with the wild type (Liang et al, 2012). Apart from these findings, well-known low-Pi responses such as increased secretory APase activity, accumulation of organic acids (Pant et al, 2015), and decreased levels of ATP (Mikulska et al, 1998) in OE lines indicate that OsHAD1 is involved in regulating PSR. Reduction in ATP content also could be caused by increased phosphatase activity of OsHAD1, as ATP was found to be the most preferred substrate of OsHAD1.…”

Section: Discussionmentioning

confidence: 92%

OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation

et al. 2017

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Phosphorus (P) deficiency limits plant growth and yield. Since plants can absorb only the inorganic form of P (Pi), a large portion of soil P (organic and inorganic P complexes) remains unused. Here, we identified and characterized a PHR2-regulated, novel low-Pi-responsive haloacid dehalogenase (HAD)-like hydrolase, While OsHAD1 is a functional HAD protein having both acid phosphatase and phytase activities, it showed little homology with other known low-Pi-responsive HAD superfamily members. Recombinant OsHAD1 is active at acidic pH and dephosphorylates a broad range of organic and inorganic P-containing substrates, including phosphorylated serine and sodium phytate. Exogenous application of recombinant OsHAD1 protein in growth medium supplemented with phytate led to marked increases in growth and total P content of Pi-deficient wild-type rice () seedlings. Furthermore, overexpression of in rice resulted in enhanced phosphatase activity, biomass, and total and soluble P contents in Pi-deficient transgenic seedlings treated with phytate as a restricted Pi source. Gene expression and metabolite profiling revealed enhanced Pi starvation responses, such as up-regulation of multiple genes involved in Pi uptake and solubilization, accumulation of organic acids, enhanced secretory phosphatase activity, and depletion of ATP in overexpression lines as compared with the wild type. To elucidate the underlying regulatory mechanisms of OsHAD1, we performed in vitro pull-down assays, which revealed the association of OsHAD1 with protein kinases such as OsNDPKs. We conclude that, besides dephosphorylation of cellular organic P, OsHAD1 in coordination with kinases may regulate the phosphorylation status of downstream targets to accomplish Pi homeostasis under limited Pi supply.

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Section: Discussionmentioning

confidence: 92%

OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation

et al. 2017

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“…It is conceivable that increase in cell division due to P application results from increase in the amount of ATP at growth centers (Chiera et al, 2004) which affect the growth characteristics. In a study with beans subjected to P stress, 50% reductions in the level of ATP in leaves and 60% reductions in total leaf area were observed (Mikulska et al, 1998). Zafar et al (2011) reported that primary whole plant response to P addition was an enhancement in shoot growth, caused by increases in leaf expansion.…”

Section: Growthmentioning

confidence: 99%

Influence of integrated phosphorus supply and plant growth promoting rhizobacteria on growth, nodulation, yield and nutrient uptake in Phaseolus vulgaris

Zafar

Abbasi

Rahim

et al. 2011

Afr. J. Biotechnol.

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To guarantee a sufficient phosphorus supply for plants, a rapid and permanent mobilization of phosphorus from the labile phosphorus fractions is necessary, because phosphorus concentrations in soil solution are generally low. Several plant growth-promoting rhizobacteria (PGPR) have shown potential to enhance phosphorus solublization and nodulation of legumes when co-inoculated with Rhizobium. This investigation was undertaken to assess the feasibility and compatibility of two mineral phosphorus fertilizers; diammonium phosphate (DAP), triple super phosphate (TSP), poultry manure (PM) and two PGPR strains on the growth, nodulation, yield, nutrient uptake and protein content of common bean (Phaseolus vulgaris) under deficient phosphorus supply. Integrated application of mineral phosphorus (P), PM and PGPR significantly increased shoot height, shoot fresh weight, shoot dry weight and leaf chlorophyll content by 67, 160, 51 and 106%, respectively, while increase in root length, root fresh weight and root dry weight was 79, 161, and 187%, respectively, over unfertilized control without PGPR application. Integrated use of different P sources and PGPR also increased number of nodules per plant, nodule fresh weight and nodule dry weight by 158, 107 and 168% over the control. Treatment with PGPR significantly increased number of pods per plant and grain yield by 224 and 96%, respectively over the control. Co-inoculation with Bradyrhizobium sp. strain MN-S and Agrobacterium sp. strain Ca-18 demonstrated twofold increase in the proportion of nitrogen (N) and P uptake as well as protein content of the common bean grain was increases by 48%. Therefore, application of PGPR with low P fertilizer rates and PM could be a viable supplementary strategy for maximum benefits in terms of cost of production and sustaining productivity.

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“…The effects of availability of P on the growth and productivity of plants had been well studied. In the study with bean (Phaseolus vulgaris) plant, Mikulska et al (1998) [5] found that after 18 days of P starvation, the accumulations of fresh weight (FW), dry weight (DW) and leaf area were only 45 to 55% and 60%, respectively, compared to control plants supplied with sufficient P. With lettuce plants, Xu et al (2004) [6] revealed that increasing solution-P concentration from 0.2 to 1.0 mM significantly increased the canopy FW by 12-25%. Some specific growth factors related to P have been identified.…”

Section: Introductionmentioning

confidence: 99%

“…Some specific growth factors related to P have been identified. For example, P increases stem strength, improves flower formation and seed production, improves crop quality; and increases resistance to diseases [5,7,8]. Results of Lynch et al (1991) [9] suggested that decrease in leaf number and size was one of the earliest and most reliable responses of bean plants to P deficiency.…”

Section: Introductionmentioning

confidence: 99%

Integrated Effects of Root-Zone Temperatures and Phosphorus Levels on Aeroponically-Grown Lettuce (Lactuca sativa L.) in the Tropics

Luo¹,

Lee²,

He³

2009

TOHORTJ

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In this study, butterhead lettuce (Lactuca sativa L. cv. Baby Butter) plants were grown at three root-zone temperatures (RZTs): 25 o C, 30 o C and ambient-RZT (A-RZT) ranging from 26 o C-42 o C while their shoots were maintained at hot ambient temperature ranging from 26 o C-42 o C. Three phosphorus (P) concentrations:-25% P (minus P, 23.25ppm), control (31.00ppm) and +25% P (plus P, 38.75ppm) were supplied to the plants at each RZT using Netherlands Standard Nutrient Solution. Interactions between RZT and P concentrations on productivity, root morphology, maximum photosynthetic O 2 evolution (P max), P uptake and its partitioning between shoot and root were studied. Lettuce plants grown with the plus P concentration at 25 o C-RZT had the highest productivity, highest P max , highest shoot and root P concentrations. However, all the root morphological parameters studied were the greatest in plants grown with the minus P at 25 o C-RZT. Higher P concentration in the solution and 25 o C-RZT resulted in higher portion of the absorbed P partitioned to the shoots. Integrated effects of RZT and P concentration on the productivity, root morphology, photosynthesis and P uptake were discussed.

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The influence of phosphate deficiency on photosynthesis, respiration and adenine nucleotide pool in bean leaves

Cited by 34 publications

References 31 publications

OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation

OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation

Influence of integrated phosphorus supply and plant growth promoting rhizobacteria on growth, nodulation, yield and nutrient uptake in Phaseolus vulgaris

Integrated Effects of Root-Zone Temperatures and Phosphorus Levels on Aeroponically-Grown Lettuce (Lactuca sativa L.) in the Tropics

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