The influence of periodate oxidation on monoclonal antibody avidity and immunoreactivity

Abraham, Ralph; Moller, Detlef; Gabel, Detlef; Senter, Peter D.; Hellström, Ingegerd; Hellström, Karl Erik

doi:10.1016/0022-1759(91)90233-6

Cited by 61 publications

(47 citation statements)

References 25 publications

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“…However, the weak signals obtained using the traditional lectin ELISA did also indicate an increased fucosylation in the HCC samples. Immunoglobulins such as IgG and IgM are known to carry fucosylated oligosaccharides and therefore there is often a need to pretreat the capturing antibodies with periodate oxidation to destroy fucose residues which could decrease antibody activity [23]. However, generally antibodies do not carry highly branched multiply fucosylated oligosaccharides, which may explain the weak binding to S2 and low background signals obtained in the reverse S2 lectin ELISA without the use of prior periodate oxidation.…”

Section: Discussionmentioning

confidence: 99%

Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma

et al. 2017

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Altered fucosylation of glycoproteins is associated with development of hepatocellular carcinoma (HCC). Lectins have been commonly used to assay changes in fucosylation of plasma glycoproteins. In the present study a recombinantly engineered form of the fucose binding lectin Aleuria aurantia (AAL) consisting of a single binding site for fucose (S2), was used to construct a reverse lectin ELISA method. Microtiter plates coated with the S2 lectin were used to capture glycoproteins from plasma samples followed by antibody detection of S2-bound fucosylated α1-acid glycoprotein (S2-bound AGP). The method was used to compare the level of S2-bound AGP in serum samples from a small cohort of patients with hepatitis, cirrhosis or HCC. Using the reverse S2 lectin ELISA it was shown that the levels of S2-bound AGP was significantly higher in HCC patients compared to non-cancer patients and that there was also a significant elevation of S2-bound AGP in HCC patients compared to cirrhosis patients. There was no correlation between the level of S2-bound AGP and total AGP concentration. The performance of S2-bound AGP in differentiating HCC from cirrhosis samples or hepatitis samples were compared to other markers. A combination of S2-bound AGP, α-fetoprotein and AGP concentration showed performances giving area under receiver operating curves of 0.87 and 0.95 respectively.

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Section: Discussionmentioning

confidence: 99%

Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma

et al. 2017

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“…It is often the case, however, that the antibody component of the immunoassay is modified by conjugation to a detectable tag, ligand, or solid support [see, for example, Abraham et al (10)]. SPR has only recently been reported to study the effect of the biotinylation of anti-LH mAbs on binding to LH in an immunometric format (11).…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance (SPR) as a Tool for Antibody Conjugate Analysis

et al. 1999

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Surface plasmon resonance (SPR) analysis was used to assess the immunoreactivity of anti-biotin (4) and anti-fluorescein (5) monoclonal antibody after conjugation with the N-hydroxysuccinimide ester of acridinium-9-carboxamide 1. Only minor changes in the apparent equilibrium dissociation constants of the antibody conjugates for their ligands resulted from the conjugation process. However, comparison of the initial binding rate of the conjugates with their ligands with those of the unmodified antibodies over a range of concentrations showed that the antibody conjugates were partially inactivated. The anti-fluorescein conjugates retained at least 90% of their immunoreactivity over the range of modification tested, while anti-biotin conjugates showed a progressive loss of reactivity with increased substitution by the label.

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“…The number of oxidized sites generated by NaIO 4 depends on its pH, temperature, reaction time and concentration of the oxidizing agent. 58 Mild oxidation conditions were chosen to preserve the immunoreactivity of the antibody. The antibody (MAB610, 50 μg) in PBS (pH 6.8) was oxidized by 0.1 mg NaIO 4 (0.47 mM) for 15 min at 4°C in the dark.…”

Section: Resultsmentioning

confidence: 99%

A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine

Bosnjakovic

Mishra

Han

et al. 2012

Analytica Chimica Acta

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A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites towards the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48 pg mL−1, which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.

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The influence of periodate oxidation on monoclonal antibody avidity and immunoreactivity

Cited by 61 publications

References 25 publications

Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma

Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma

Surface Plasmon Resonance (SPR) as a Tool for Antibody Conjugate Analysis

A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine

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