The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms

Zakrzewski, Wojciech; Nowicka, J; Pajączkowska, Magdalena; Szymonowicz, Maria; Targońska, Sara; Sobierajska, Paulina; Wiglusz, Katarzyna; Dobrzyński, W; Lubojański, Adam; Fedorowicz, Sebastian; Rybak, Zbigniew; Wiglusz, Rafał J.

doi:10.3390/nano10101997

Cited by 11 publications

(5 citation statements)

References 51 publications

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“…Hydroxyapatite powder is soluble in olive oil and the food-grade olive oil is harmless to human health. Olive oil has been used in preparations of hydroxyapatite 37,38 and as an additive agent to hydroxyapatite 39 . Commercial toothpastes often contain water as an ingredient; however, in a homemade toothpaste, water provides a suitable environment for the growth and proliferation of microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Effects of Homemade Nano-Hydroxyapatite and Olive Oil Paste on Remineralization of Early Caries Lesions

et al. 2022

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Nano-hydroxyapatite (nHAP) particles are a new generation of materials reported to remineralize enamel lesions. The purpose we aimed was to compare the in vitro effects of fluoride gel, sodium fluoride toothpaste, and homemade nHAP paste on remineralizing artificial early enamel caries. Methods: Incipient caries were induced in 21 extracted, sound premolar teeth by storing each specimen in a demineralization solution for 72 hours, followed by pH cycling. The samples (n= 7, each) were then treated with 2% neutral fluoride gel, 0.25% sodium fluoride toothpaste, or homemade nHAP paste, comprising a mixture of nHAP powder and olive oil. After demineralization and remineralization, the results were compared using the DIAGNOdent pen (KaVo, Germany). The data were statistically analyzed using paired t-tests and a one-way ANOVA test. Outcomes: The degree of demineralization in each of the three groups (fluoride gel group, 15.71; sodium fluoride dentifrice group, 15.28; nHAP paste group, 16.71) was significantly elevated compared to baseline (3, 2.5, 2.28, respectively); however, no significant difference was observed between the remineralization readings in each of the three groups (6, 7, 5.5, respectively) (p > 0.05). In conclusion, we concluded that the homemade nHAP paste had a beneficial effect on the remineralization of initial enamel caries lesions.

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Section: Resultsmentioning

confidence: 99%

Effects of Homemade Nano-Hydroxyapatite and Olive Oil Paste on Remineralization of Early Caries Lesions

et al. 2022

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“…In order to study the structural features of the prepared niosomes compared to free OL, the FTIR spectra of OL, empty niosomes, and OL/NSs were obtained (Figure 3). The FTIR spectrum of ozonated olive oil (Figure 3A) showed four major peaks at 3208.9 cm −1 , (OH stretching), 2670.8 cm −1 (C-H stretching), 1490.7 cm −1 (C-C stretching), and 1211.0 cm −1 (C-O stretching) [29]. The FTIR spectrum of plain niosomes (Figure 3B) showed four characteristic peaks at 2850.4 cm −1 (C-H stretching), 1650.8 cm −1 (C=O stretching), 1467.6 cm −1 (C-H bending), and 1093 cm −1 (C-O stretching) [30].…”

Section: Morphological and Chemicalmentioning

confidence: 99%

Ozonated Olive Oil: Enhanced Cutaneous Delivery via Niosomal Nanovesicles for Melanoma Treatment

et al. 2022

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Ozonated olive oil (OL) combines the therapeutic effects of both ozone and olive oil. However, it suffers from limited water solubility and poor transdermal permeation, which hinder its application in melanoma treatment. Nanocarrier host molecules, such as niosomes, were used to improve the water solubility, transdermal permeation, and anticancer effect of hydrophobic compounds. This study aims to design and optimize a niosomal vesicular nanoplatform loaded with OL (OL/NSs) to improve OL’s skin permeation and anti-melanoma effect. In this regard, OL was prepared and characterized by evaluating its chemical properties (acid, peroxide, and iodine values) and fatty acid composition using gas chromatography. Then, OL/NSs were developed using the thin film hydration method employing cholesterol, Span 60, and Tween 60 at five different molar ratios. The optimized niosomes had an average diameter of 125.34 ± 13.29 nm, a surface charge of −11.34 ± 4.71 mV, and a spherical shape. They could entrap 87.30 ± 4.95% of the OL. OL/NSs showed a 75% sustained oil release over 24 h. The skin permeation percentage of OL/NSs was 36.78 ± 3.31 and 53.44 ± 6.41% at 12 and 24 h, respectively, three times higher than that of the free OL (11.50 ± 1.3 and 17.24 ± 2.06%, at 12 and 24 h, respectively). Additionally, the anticancer activity of the developed niosmal formulation, when tested on human melanoma cells (A375), was double that of the free OL; the IC50 of the OL/NSs was 8.63 ± 2.8 μg/mL, and that of the free OL was 17.4 ± 3.7 μg/mL. In conclusion, the encapsulation of ozonated olive oil in niosomes enhanced its water solubility, skin permeation, and anticancer activity and thus may represent potent natural chemotherapy in treating melanoma.

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“…The high bioactivity of nanohydroxyapatite results from its similarity to bone apatite as well as its strong ion exchange affinity. In addition, its biological properties are determined by the size and morphology of molecules, the type of ionic impurities in the crystal lattice and the Ca/P molar ratio [59,60], resulting in its hexagonal system crystallization [61]. In addition, its biological properties are determined by the size and morphology of the molecules (Figure 2).…”

Section: Hydroxyapatite (Hap)mentioning

confidence: 99%

Nanomaterials Application in Endodontics

et al. 2021

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In recent years, nanomaterials have become increasingly present in medicine, especially in dentistry. Their characteristics are proving to be very useful in clinical cases. Due to the intense research in the field of biomaterials and nanotechnology, the efficacy and possibilities of dental procedures have immensely expanded over the years. The nano size of materials allows them to exhibit properties not present in their larger-in-scale counterparts. The medical procedures in endodontics are time-consuming and mostly require several visits to be able to achieve the proper result. In this field of dentistry, there are still major issues about the removal of the mostly bacterial infection from the dental root canals. It has been confirmed that nanoparticles are much more efficient than traditional materials and appear to have superior properties when it comes to surface chemistry and bonding. Their unique antibacterial properties are also promising features in every medical procedure, especially in endodontics. High versatility of use of nanomaterials makes them a powerful tool in dental clinics, in a plethora of endodontic procedures, including pulp regeneration, drug delivery, root repair, disinfection, obturation and canal filling. This study focuses on summing up the current knowledge about the utility of nanomaterials in endodontics, their characteristics, advantages, disadvantages, and provides a number of reasons why research in this field should be continued.

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The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms

Cited by 11 publications

References 51 publications

Effects of Homemade Nano-Hydroxyapatite and Olive Oil Paste on Remineralization of Early Caries Lesions

Effects of Homemade Nano-Hydroxyapatite and Olive Oil Paste on Remineralization of Early Caries Lesions

Ozonated Olive Oil: Enhanced Cutaneous Delivery via Niosomal Nanovesicles for Melanoma Treatment

Nanomaterials Application in Endodontics

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