The influence of overfilling of microsamples on the results of a Complete Blood Count

Kościelniak, Barbara; Kowalczyk, Paulina; Manda-Handzlik, Aneta; Tomasik, Przemysław

doi:10.1111/ijlh.12392

Cited by 1 publication

(2 citation statements)

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“…It was shown that the overfilling may lead to the micro-clots formation and to decrease in a number of morphotic elements of blood [17,18]. Moreover, in a previous study, we observed that excess collection of blood volume resulted in a decrease of a number of erythrocytes, concentration of hemoglobin, hematocrit and mean corpuscular volume as well as in increase in the number of platelets [6].…”

Section: Discussionmentioning

confidence: 80%

“…In the case of CBC measurement, one of the requirements is that blood must fill the tube to volume recommended by the manufacturer and adequate blood to anticoagulant ratio preserved [3,5]. It was shown in previous studies that improper filling of microtubes results of the impossibility of analysis in the case of significant underfilling and false results in the case of significant overfilling [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Blood Microsampling for Complete Blood Count: Take Heed of Preanalytical Errors

Kościelniak

Zając

Tomasik

2017

Int. J. Child Health Nutr.

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Background: The credibility of the result of a complete blood count is closely connected with the preanalytical phase.Objectives: This study evaluated accordance of filling of microtubes with manufacturer's recommendation and assessed the effect of storage of overfilled and underfilled samples on the results of complete blood count.Design and Methods: Volume of blood samples collected into microtubes in the wards of the University Children's Hospital in Cracow during one month was analyzed. In the stability studies, overfilled and underfilled samples stored at ambient temperature were analyzed at 1, 2, 3 and 12 hours after phlebotomy. The analysis was made using the SYSMEX XT-1800i analyzer.Results: More than half of the analyzed samples were incorrectly filled. 63% of the samples were filled above the manufacturer's recommended volume and 15% of test-tubes were filled below the recommendation. We observed differences between collected blood volume in accordance to the age of patients (p=0.001). The storage of overfilled and underfilled microtubes for complete blood count for 1,2,3 and 12 h at room temperature had no effect on the results of this test. Conclusion:Medical staff does not follow the instructions of the manufacturers. It might lead to a decrease of the quality and credibility of the results.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%