The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode

Zhang, Long; Yu, Deshui; Liu, Yanfang; Du, Nana; Tao, Yanduo; Mei, Lijuan; Guo, Zhimou; Liang, Xinmiao

doi:10.1016/j.aca.2014.12.057

Cited by 8 publications

(3 citation statements)

References 29 publications

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“…Due to the unique structural characteristics of isoquinoline alkaloids, influencing chromatographic retention behavior, IEX chromatography (with the SCX column) was used to separate fractions from fraction 5. The SCX column displays good hydrophobic action and cation‐exchange properties , and the surface‐bound sulfonic acid groups display favorable peak profiles and retention of isoquinoline alkaloids in C. impatiens . The SCX column also has excellent stability , thus maintaining the consistency of the 1D preparative fractions.…”

Section: Resultsmentioning

confidence: 99%

“…The SCX column displays good hydrophobic action and cation‐exchange properties , and the surface‐bound sulfonic acid groups display favorable peak profiles and retention of isoquinoline alkaloids in C. impatiens . The SCX column also has excellent stability , thus maintaining the consistency of the 1D preparative fractions.…”

Section: Resultsmentioning

confidence: 99%

“…We selected two separation methods for 2D‐LC, RPLC, and IEX chromatography . Increased sample loading is required during separation, and sample loading is related to sample solubility in the mobile phase.…”

Section: Introductionmentioning

confidence: 99%

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Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography

Pan

Shen²,

et al. 2019

J of Separation Science

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We established a two-dimensional strong cation exchange/reversed-phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium-pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22-29 for reversed-phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22-29) were further separated, and those of low resolution were isolated via two-dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off-line two-dimensional strong cation exchange/reversed-phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%