In order to contribute to the solution of controlling the auto-ignition in a Homogeneous Charge Compression Ignition (HCCI) engine, parameters linked to External Gas Recirculation (EGR) seem to be of particular interest. Experiments performed with EGR present some difficulties in interpreting results using only the diluting and thermal aspect of EGR. Lately, the chemical aspect of EGR is taken more into consideration, because this aspect causes a complex interaction with the dilution and thermal aspects of EGR. This paper studies the influence of EGR on the auto-ignition process and particularly the chemical aspect of EGR.The diluents present in EGR are simulated by N 2 and CO 2 , with dilution factors going from 0 to 46 vol%. For the chemically active species that could be present in EGR, the species CO, The fuels used for the auto-ignition are n-heptane and PRF40. It appeared that CO, in the investigated domain, did not influence the ignition delays, while NO had two different effects.At concentrations up until 45 ppm, NO advanced the ignition delays for the PRF40 and at higher concentrations, the ignition delayed. The influence of NO on the auto-ignition of nheptane seemed to be insignificant, probably due to the higher burn rate of n-heptane. CH 2 O seemed to delay the ignition. The results suggested that especially the formation of OH radicals or their consumption by the chemical additives determine how the reactivity of the auto-ignition changed.