The influence of NK cell-mediated ADCC: Structure and expression of the CD16 molecule differ among FcγRIIIa-V158F genotypes in healthy Japanese subjects

Oboshi, Wataru; Watanabe, Tôru; Matsuyama, Yuumi; Kobara, Ayana; Yukimasa, Nobuyasu; Ueno, Ichiro; Aki, Kensaku; Tada, Tomoki; Hosoi, Eiji

doi:10.1016/j.humimm.2015.11.001

Cited by 24 publications

(29 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may limit ADCC activity despite higher levels of CMV antibodies. A polymorphism in the gene encoding CD16 (rs396991) affects surface expression . Carriage of the V allele associates with better clinical and biological responses to monoclonal antibody therapies .…”

Section: Discussioncontrasting

confidence: 96%

“…An F176V change, denoted rs396991, increases binding affinity for IgG and affects CD16 expression on NK cells . Alleles of two SNPs in FCRG3A (rs4656317 and rs12071048) in linkage disequilibrium with rs396991 also associated with levels of FCRG3A transcripts and CD16 expression on NK cells . When RTR and controls were analyzed together, rs396991 genotype frequencies were FF (38%), VF (44%), and VV (18%).…”

Section: Resultsmentioning

confidence: 99%

“…15 Alleles of two SNPs in FCRG3A (rs4656317 and rs12071048) in linkage disequilibrium with rs396991 also associated with levels of FCRG3A transcripts and CD16 expression on NK cells. 16,17 When RTR and controls were analyzed together, rs396991 genotype frequencies were FF (38%), VF (44%), and VV (18%). CD16 expression (assessed as MFI) was lower in those with FF genotypes ( Figure 4A).…”

Section: A Genetic Determinant Of Cd16 Expression Affects Nk Cell Amentioning

confidence: 99%

“…A polymorphism in the gene encoding CD16 (rs396991) affects surface expression. 17 ADCC responses against CMV were primarily mediated by conventional FcRγ+ NK cells. This is in contrast to previous papers where FcRγ-NK cells displayed higher effector function including the production of cytokines and expression of CD107a.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Functional and clinical consequences of changes to natural killer cell phenotypes driven by chronic cytomegalovirus infections

Lee

Doualeh

Affandi

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

Cytomegalovirus (CMV) infections may affect natural killer (NK) cells and are implicated in age‐related disorders—notably poor vascular endothelial function. Changes may be greater in renal transplant recipients (RTR) as they have a high burden of CMV and may influence antibody‐dependent cellular cytotoxicity (ADCC) responses to viral antigen. We obtained blood mononuclear cells from RTR stable after transplantation (n = 27) and age‐ and sex‐matched controls (n = 28). Natural killer (NK) cells were assessed for expression of CD107a or TNF‐α, after stimulation with autologous antibodies bound to CMV glycoprotein B (measuring ADCC) or anti‐CD16 (measuring NK cell activation). Alleles of FCRG3A (encoding CD16; rs396991) were determined by the Taqman assay. The vascular endothelial function was assessed using flow‐mediated dilatation (FMD) of the brachial artery. Proportions of NK cells expressing CD16 ex vivo were lower in RTR. Frequencies of NK cells expressing NKG2C or LIR‐1 or lacking FcRγ were highest in CMV‐seropositive RTR. ADCC was affected by rs396991 genotype and CMV gB antibody levels, but not by RTR status or detection of CMV DNA in plasma. Responses of FcRγ‐NK cells to anti‐CD16 were lower compared to FcRγ+ NK cells. Increased percentages of LIR‐1 + and FcRγ− NK cells correlated with lower FMD. In summary, CMV evokes substantial and similar ADCC responses in CMV seropositive RTR and controls. The equivalence may reflect higher titers of CMV reactive antibody in RTR, as NK responses stimulated by ligation of CD16 were lower. NK cells that were LIR‐1 + and/or FcRγ− were induced by CMV and correlated inversely with vascular endothelial function.

show abstract

Section: Discussioncontrasting

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: A Genetic Determinant Of Cd16 Expression Affects Nk Cell Amentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Functional and clinical consequences of changes to natural killer cell phenotypes driven by chronic cytomegalovirus infections

Lee

Doualeh

Affandi

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…+ lymphocytes although the expression pattern of NK cells includes variety of receptors including killer immunoglobulin-like receptors (KIR) [38], CD16 (Fcγ-receptor III), which mediates antibody-dependent cellular cytotoxity [25,32], natural killer group (NKG) receptors, cytotoxic receptors NKp30, NKp44, NKp46 [20]. NK cells differentiation and development is connected with the consequent change of repertoire of receptors present in the cell and their acquisition of functional properties.…”

Section: Cd56mentioning

confidence: 99%

PECULIARITIES OF NK CELLS DIFFERENTIATION: CD56dim AND CD56bright NK CELLS AT PREGNANCY AND IN NON-PREGNANT STATE

Михайлова¹,

Белякова²,

Selkov³

et al. 2017

Med. immunol.

View full text Add to dashboard Cite

QPY/RAH haplotypes of the GZMB gene are associated with natural killer cell cytotoxicity

et al. 2017

View full text Add to dashboard Cite

Granzyme B (GzmB) is a component of cytolytic granules within NK cells and is involved in several pathologies. It has previously been reported that there are three non-synonymous coding SNPs (rs8192917; Q48R, rs11539752; P88A, and rs2236338; Y245H) in the GZMB gene and that the QPY/RAH allele was clustered together close to the C-terminal α-helix. However, it is unknown whether the function of GzmB produced from NK cells is influenced by QPY/RAH polymorphism. The authors investigated the distribution of QPY/RAH polymorphism of the GZMB gene in a Japanese population (n = 106), and the involvement of Q48R polymorphism in NK cell cytotoxicity, degranulation, and production of GzmB. A strong linkage disequilibrium was observed among these SNPs, and NK cell cytotoxicity was influenced by rs8192917 (Q48R). Moreover, it found that R-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. The authors expect that these new informations about QPY/RAH polymorphism of the GZMB gene could help to assess the impact of NK cell cytotoxicity in several pathologies and aid their treatment.

show abstract

The influence of NK cell-mediated ADCC: Structure and expression of the CD16 molecule differ among FcγRIIIa-V158F genotypes in healthy Japanese subjects

Cited by 24 publications

References 38 publications

Functional and clinical consequences of changes to natural killer cell phenotypes driven by chronic cytomegalovirus infections

Functional and clinical consequences of changes to natural killer cell phenotypes driven by chronic cytomegalovirus infections

PECULIARITIES OF NK CELLS DIFFERENTIATION: CD56dim AND CD56bright NK CELLS AT PREGNANCY AND IN NON-PREGNANT STATE

QPY/RAH haplotypes of the GZMB gene are associated with natural killer cell cytotoxicity

Contact Info

Product

Resources

About