The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro

Ciesielska-Figlon, Klaudia; Daca, Agnieszka; Kokotkiewicz, Adam; Łuczkiewicz, Maria; Zabiegała, Bożena; Witkowski, Jacek M.; Lisowska, Katarzyna A.

doi:10.1016/j.biopha.2022.113349

Cited by 11 publications

(12 citation statements)

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“…On the other hand, “Roman coriander” ( Nigella sativa L., Ranunculaceae ) essential oil has not been found to have any beneficial or detrimental effects on neutrophil activities [ 78 ]. However, recent research shows that thymoquinone, the primary component of the seed essential oil of N.sativa , has the ability to inhibit in vitro the proliferation of CD4+ and CD8+ lymphocytes and to induce apoptosis in a dose-dependent manner [ 79 ]. A recent evaluation of the biological activities of Juniperus spp.…”

Section: Discussionmentioning

confidence: 99%

Immune Defences: A View from the Side of the Essential Oils

et al. 2023

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The use of essential oils is increasingly being investigated among new therapeutic approaches based on medicinal plants and their extracts. With the wide use of synthetic and semi-synthetic antimicrobial drugs, the spread of drug-resistant clinical isolates has increased, and research is directed towards natural products, such as essential oils, as useful antimicrobial resources. In the context of a prospective infection, we compared the impact of essential oils and common antimicrobial agents on the microbicidal activity of human phagocytes. Here, we present the results of our decades-long investigation into the effectiveness of thyme red oil (26.52% thymol chemotype), tea tree oil (TTO), and Mentha of Pancalieri [(Mentha x piperita (Huds) var. officinalis (Sole), form rubescens (Camus) (Lamiaceae)] essential oils on human polymorphonuclear leukocytes (PMNs) capacity to kill clinical strains of Candida albicans and C. krusei when compared to three antifungal drugs used to treat candidiasis (fluconazole, anidulafungin, and caspofungin) These essential oils demonstrate antifungal drug-like and/or superior efficacy in enhancing intracellular killing by PMNs, even at subinhibitory concentrations. Our results are compared with data in the literature on essential oils and immune system interactions. This comparison would aid in identifying therapeutic solutions to the increasingly prevalent antibiotic resistance as well as filling in any remaining knowledge gaps on the bioactivity of essential oils.

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Section: Discussionmentioning

confidence: 99%

Immune Defences: A View from the Side of the Essential Oils

et al. 2023

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“…Our findings showed that the proliferation of CD4 + and CD8 + T cells from healthy women and HT patients is significantly inhibited mainly in the presence of the lowest (1:10) NSEO dilution; the number of cell divisions reduced, and the percentage of dividing cells decreased. In our previous work, we used a different stimulation protocol in which NSEO was added simultaneously with anti-CD3 antibody, which resulted in a much more significant reduction in the percentage of proliferating T cells from healthy people, observed even at higher NSEO dilutions (1:50 and 1:100) [ 29 ]. Thus, adding NSEO before stimulation has a much stronger effect, probably due to the inhibition of T cell activation through the TCR/CD3 complex.…”

Section: Discussionmentioning

confidence: 99%

“…NSEO isolation and GC analysis were performed in the Department of Pharmacognosy of the Medical University of Gdańsk and the Department of Analytical Chemistry of the Gdańsk University of Technology. The details of the processes and parameters are described in a previous paper [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

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The Impact of Nigella sativa Essential Oil on T Cells in Women with Hashimoto’s Thyroiditis

et al. 2023

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Background: Hashimoto’s thyroiditis (HT) is an autoimmune disease mediated by T cells. It is characterized by the presence of thyroid autoantibodies in the serum, such as anti-thyroid peroxidase antibodies (TPO-Ab) and anti-thyroglobulin antibodies (TG-Ab). The essential oil extracted from Nigella sativa seeds is rich in bioactive substances, such as thymoquinone and cymene. Methods: Therefore, we examined the effect of essential oil from Nigella sativa (NSEO) on T cells from HT patients, especially their proliferation capacity, ability to produce cytokines, and susceptibility to apoptosis. Results: The lowest ethanol (EtOH) dilution (1:10) of NSEO significantly inhibited the proliferation of CD4+ and CD8+ T cells from HT patients and healthy women by affecting the percentage of dividing cells and the number of cell divisions. In addition, 1:10 and 1:50 NSEO dilutions induced cell death. Different dilutions of NSEO also reduced the concentration of IL-17A and IL-10. In healthy women, the level of IL-4 and IL-2 significantly increased in the presence of 1:10 and 1:50 NSEO dilutions. NSEO did not influence the concentration of IL-6 and IFN-γ. Conclusions: Our study demonstrates that NSEO has a strong immunomodulatory effect on the lymphocytes of HT patients.

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“…Some essential oils may stimulate the proliferation of immune-competent cells, including polymorphonuclear leukocytes, macrophages, dendritic cells, natural killer cells, and B and T lymphocytes [ 38 , 39 ]. For instance, constituents of essential oils produced by Eucalyptus stimulate phagocytosis by macrophages [ 40 ], while Nigella sativa essential oil stops the proliferation of CD4+ and CD8+ lymphocytes in vitro [ 41 ].…”

Section: Influence Of Selected Essential Oils On the Immune Systemmentioning

confidence: 99%

How to Naturally Support the Immune System in Inflammation—Essential Oils as Immune Boosters

Grazul,

Kwiatkowski,

Hartman

et al. 2023

Biomedicines

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Efficient functionality of the immune system is needed to fight against the development of infectious diseases, including, among others, serious recurrent chronic infections. Research has shown that many modern common diseases, such as inflammatory bowel diseases and cardiovascular diseases, e.g., thromboembolism, cancer, obesity, or depression, are connected with inflammatory processes. Therefore, new, good stimulators of the immune system’s response are sought. They include synthetic compounds as well as biological preparations such as lipopolysaccharides, enzymes, bacterial metabolites, and secondary metabolites of plants, demonstrating a multidirectional effect. Essential oils are characterized by many invaluable activities, including antimicrobial, antioxidant, anti-inflammatory, and immunostimulating. Essential oils may stimulate the immune system via the utilization of their constituents, such as antibodies, cytokines, and dendritic cells. Some essential oils may stimulate the proliferation of immune-competent cells, including polymorphonuclear leukocytes, macrophages, dendritic cells, natural killer cells, and B and T lymphocytes. This review is focused on the ability of essential oils to affect the immune system. It is also possible that essential oil components positively interact with recommended anti-inflammatory and antimicrobial drugs. Thus, there is a need to explore possible synergies between essential oils and their active ingredients for medical use.

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The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro

Cited by 11 publications

References 30 publications

Immune Defences: A View from the Side of the Essential Oils

Immune Defences: A View from the Side of the Essential Oils

The Impact of Nigella sativa Essential Oil on T Cells in Women with Hashimoto’s Thyroiditis

How to Naturally Support the Immune System in Inflammation—Essential Oils as Immune Boosters

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