The influence of nanodiamond on the oxygenation states and micro rheological properties of human red blood cells &lt;italic&gt;in vitro&lt;/italic&gt;

Lin, Ying‐Chih; Tsai, Lin-Wei; Perevedentseva, E.; Chang, Hsin-Hou; Lin, Chih‐Wei; Sun, Der-Shan; Луговцов, А.Е.; Priezzhev, Alexander V.; Jani, Mona; Cheng, Chia‐Liang

doi:10.1117/1.jbo.17.10.101512

Cited by 49 publications

(52 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also have compared the effect of ND and NDberberine complex on RBC function using in situ Raman spectroscopic measurements; method has been presented previously [16,17]. RBC Raman spectra are determined by Hb inside and are very sensitive to the oxygenation and spin state dynamics of the heme (the active center of Hb) due to a selective enhancement of the Raman active vibrations in different oxygenation states [55].…”

Section: Resultsmentioning

confidence: 99%

“…Raman spectroscopic analysis of RBC was performed according to methods described previously [16,17]. For Raman spectroscopic investigation of ND-berberine and berberine interaction with RBC, the Raman spectra of RBC determined by hemoglobin (Hb) were measured using a Raman spectrometer (T64000, Jobin Yvon, France) with 633 nm excitation wavelength laser.…”

Section: Cells Raman Measurements and Confocal Fluorescence Imagingmentioning

confidence: 99%

“…Functionality of proteins attached on ND (e.g. enzyme [11], hemoglobin [16,17], lipoproteins [12], different drugs [8][9][10][18][19][20]) has been investigated. Difference in mechanism of interaction of nanodiamond conjugates and pure drug with the target objects can modify the treatment [18], particularly, to overcome the chemoresistance of tumor [19,20].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Lin¹,

Lin²,

Tsai³

et al. 2017

JBPE

Self Cite

View full text Add to dashboard Cite

Abstract. The natural isoquinoline alkaloid berberine has been demonstrated for its significant activity against a variety of deceases caused by bacterial and parasite infections. Nanodiamond (ND) has also been proven to have great potential for drug delivery. In this work, the complex of ND with berberine is constructed through surface functionalized ND, aiming for drug delivery applications. Spectroscopic analysis was performed on the complex interaction with human lung alveolar carcinoma epithelial cell (A549) and red blood cell (RBC) in comparison with berberine in solution. The studied cells were treated with carboxylated ND, ND-berberine complex and berberine, respectively. Their distributions in cells structures are visualized using ND and berberine's fluorescence signals for detection. The distribution of ND and ND-berberine complex differs with the distribution of berberine introduced in solution form. The results show that the ND with attached drug molecules can act as a drug carrier allowing controllable localization of the drug into the cell. This result can have implication in drug delivery, release and delivery tracing applications. © 2017 Journal of Biomedical Photonics & Engineering.Keywords: Nanodiamond; Berberine; Bio-Applications; Medical Applications; Fluorescence; Surface Functionality; Imaging; Drug delivery; Nanobiotechnology. targeted nanoparticles as specific Helicobacter pylori eradication therapy: in vitro and in vivo study," Nanomedicine (Lond) 10(1), 57-71 (2015). 48. P. R. Vuddanda, A. Mishra, S. K. Singh, and S. Singh, "Development of polymeric nanoparticles with highly entrapped herbal hydrophilic drug using nanoprecipitation technique: an approach of quality by design," Pharm. Dev. Technol.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Cells Raman Measurements and Confocal Fluorescence Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Lin¹,

Lin²,

Tsai³

et al. 2017

JBPE

Self Cite

View full text Add to dashboard Cite

show abstract

“…We suppose that these deformed individuals would not hatch. After 20 days of incubation (HH stages [44][45], chickens were completely developed and yolk sacs almost totally enclosed by the body cavity. Only in the rGO group were yolk sacs only partially enclosed by the body cavity.…”

Section: Evaluation Of Embryotoxicitymentioning

confidence: 99%

“…Raman spectra did not show any changes in RBCs, hemoglobin, or hemoglobin-oxygenation state. 45 On the other hand, Puzyr et al 46,47 demonstrated that ND particles synthesized by the explosion method induced death in human white BCs and hemolysis of erythrocytes in vitro. The authors suggested that the damaging effects were due to 1) direct binding of ND to cell-membrane proteins, which may cause irreversible inhibition of their functions, and 2) an imbalance of electrolytic and osmotic equilibrium caused by adsorption of blood plasma protein components to ND particles.…”

mentioning

confidence: 99%

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Kurantowicz

Sawosz

Halik

et al. 2017

IJN

View full text Add to dashboard Cite

In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 μg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

show abstract

Biocompatibility of Nanodiamonds

2018

Fluorescent Nanodiamonds

View full text Add to dashboard Cite

The influence of nanodiamond on the oxygenation states and micro rheological properties of human red blood cells <italic>in vitro</italic>

Cited by 49 publications

References 50 publications

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Biocompatibility of Nanodiamonds

Contact Info

Product

Resources

About