The Influence of Microscopic Fungi on Chromated Galvanized Zinc Coatings

Lugauskas, Albinas; Prosyčevas, Igoris; Selskienė, Aušra; Demčenko, Irina; Narkevičius, Algirdas; Bučinskienė, Dalia; Binkauskienė, Elena

doi:10.4236/eng.2010.212125

Cited by 3 publications

(4 citation statements)

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“…Electrodes were sterilized in 70 vol% alcohol for 2 hours and then stored in clean bench until use. Lugauskas and others (2009) 11 Low carbon steel, composition (wt%): 0.05-0.12 C, 0.003-0.10 Cu, and <0.07 P Coupons were treated with a fine suspension of Mg(OH) 2 and high purity acetone to minimize initial contamination of the surface with nutritive substances from the environment. Miyanaga and others (2007) 14 Carbon steel coupons composition (wt%): 99.71 Fe, 0.03 C, 0.01 Si, 0.19 Mn, 0.013 P, 0.0017 N, 0.026 Al…”

Section: Methodsmentioning

confidence: 99%

“…approximately 4000 times the residual chlorine level required to treat natural seawater) [7][8][9] for 30 minutes, followed by overnight treatment with glutaraldehyde. 10 Others employed fine Mg(OH) 2 suspensions and high-purity acetone; 11 overnight immersion in 70 vol% ethanol, followed by drying aseptically in air; 12 9 h heat treatments at 130 • C in glass beakers covered with aluminum foil; 13 or the use of ultraviolet light. 14 It is important to mention that the methods listed in Table I correspond roughly to the same number of papers explicitly describing the sterilization procedures in the methodology section of the manuscript.…”

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confidence: 99%

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Efficacy of Sterilization Methods and Their Influence on the Electrochemical Behavior of Plain Carbon Steel

Giai

Ortiz

Kappes

et al. 2016

J. Electrochem. Soc.

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Minimizing contamination of control treatments in microbiologically influenced corrosion (MIC) studies is of critical importance. Metal sterilization procedures should not alter the surface nor affect the inherent susceptibility of the metal to corrosion while adequately deactivating biological activity. However, there is no consensus in the literature regarding such procedures due to, in part, the lack of a universally accepted methodology. This investigation evaluates various sterilization methods for carbon steel concerning practicality, efficacy, and effects on the electrochemical response of the metal. Three sterilization procedures using i) dry heat, ii) ethanol, or iii) glutaraldehyde as sterilizing agents were evaluated. Even though all sterilization approaches were equally effective in eliminating microorganisms and spores from the metal surface, dry heating at 170 • C in an inert atmosphere was identified as the most convenient sterilization method regarding practicality and consistency in the electrochemical response of the metal. Sterilization of carbon steels in 75 vol% ethanol and glutaraldehyde, as well as alcohol followed by flaming, is discouraged given the large dispersion in corrosion response caused by the exposure to the sterilization media. Microbiologically influenced corrosion (MIC) studies deal with the role microorganisms have on electrochemical processes leading to corrosion.1-3 As bacteria and fungi interact with the metal surface and its environment, they are able "to initiate, facilitate or accelerate the corrosion reaction without changing its electrochemical nature."4 The ubiquitous nature of some microorganisms can present a challenge to MIC studies, where contamination with species of diverse metabolic capabilities can alter the result of the experiments. Therefore, minimizing contamination from foreign microorganisms to maintain a microbial community that properly reflects the desired microbial composition (i.e. these being an environmental sample or a specifically defined community) is of critical importance in MIC research.Most of the components used in an MIC study (i.e. glassware, reactors or electrochemical cells, and solutions) can be sterilized following standard sterilization procedures 5 used by microbiologists; however, there is no consensus regarding sterilization procedures for metal samples in the literature. In the ideal situation, the sterilization methodology should kill all microorganisms and spores on the metal, but it should not alter its surface nor affect the inherent susceptibility of the metal on corrosion.In a literature survey of over 200 papers dealing with MIC of carbon and low alloy steels, there were over 20 different procedures to sterilize metal samples, Table I. Some of the methods described in the literature range from autoclaving steel stubs inside of watertight containers, submerging the stubs in 70 vol% ethyl alcohol and flaming them before inoculation, 6 to immersion in a 2000 ppm chlorine solution (i.e. approximately 4000 times the residual...

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Efficacy of Sterilization Methods and Their Influence on the Electrochemical Behavior of Plain Carbon Steel

Giai

Ortiz

Kappes

et al. 2016

J. Electrochem. Soc.

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“…The fungi (listed in Table ) of different functional morphology from our culture collection were used during this study . The fungi species were isolated from metal plates which were exposed at three climatic study ground of Lithuania (for 2 years): marine (M) – dunes on the shore of the Baltic Sea (Neringa), rural–agrarian (R) – Kulionys village in the district of Moletai and the industrial (I) zone of Vilnius as described in . The investigation sites were installed according to the requirements of ISO 8565:1992(E) standard.…”

Section: Methodsmentioning

confidence: 99%

“…The PANI samples were exposed to Petri dishes with a pure fungi culture and incubated at 26 °C. Biofilm‐modified surface coverage was evaluated using a five‐point corrosion test [EN ISO 10289:1999] and checked using a standard four‐point scheme after 7, 14, 32 and 56 days by a light microscope MVS‐10 (Russia) . Note that the electrochemical characteristics were evaluated after incubation for 56 days.…”

Section: Methodsmentioning

confidence: 99%

The mycological effect on morphological, electrochemical and redox properties of the polyaniline surface

Binkauskienė

Lugauskas

Bukauskas

2013

Surface & Interface Analysis

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Biofilm formation with eight different microscopic fungi on the polyaniline surface and its effect on the morphological, electrochemical and redox properties have been studied. The investigations on selected Chrysosporium merdarium and Rhizomucor pusillus fungi behavior were performed. Conspicuous difference in the oxidation level, redox activity and oxalate impurities at the polymer surface layer treated with Chrysosporium merdarium and Rhizomucor pusillus was confirmed. The electrochemical behavior of the modified electrode was characterized using the cyclic voltammetry technique. The chemical composition of treated surface was determined by X‐ray photoelectron spectroscopy. For the estimation of conductive polymer morphology and roughness, the method of optical and scanning probe microscopy was applied, which showed that the polyaniline surfaces treated with Chrysosporium merdarium are homogenous and smoother than the polyaniline surfaces treated with Rhizomucor pusillus. Water contact angle measurements confirmed the effect of microbial growth on the properties of the polyaniline surface. Copyright © 2013 John Wiley & Sons, Ltd.

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