The influence of microchannels on neurite growth and architecture

Mahoney, Melissa J.; Chen, Ruth R.; Tan, Jian; Saltzman, W. Mark

doi:10.1016/j.biomaterials.2004.03.015

Cited by 206 publications

(193 citation statements)

References 28 publications

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“…The development of polarized morphology follows cell adhesion, and continues for days and weeks before maturation, introducing additional challenges for engineering the neurites after the establishment of the patterns of adhesion. Microfluidics [33][34][35][36] has been used for positioning neuronal somata. However, culturing neurons within microfluidic chips over long terms is a challenge, possibly due to limited nutrient exchange.…”

Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

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By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.

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Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

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“…Overall, neurites show increasing alignment as features decrease in width from 500 to 5 mm [4,8,32,59,60]. To take advantage of the guidance effect of the aforementioned topographical features micro and nano-scale structures are currently being incorporated into a number of NGCs designs with the luminal walls displaying longitudinally ordered guidance structures to regenerating axons and similarly to that of migrating and proliferating SCs (table 3).…”

Section: Luminal Wall Guidance Features: Enhancing Porosity and Incrementioning

confidence: 99%

A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

Daly

Yao

Zeugolis

et al. 2011

J. R. Soc. Interface.

492

508

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Microsurgical techniques for the treatment of large peripheral nerve injuries (such as the gold standard autograft) and its main clinically approved alternative-hollow nerve guidance conduits (NGCs)-have a number of limitations that need to be addressed. NGCs, in particular, are limited to treating a relatively short nerve gap (4 cm in length) and are often associated with poor functional recovery. Recent advances in biomaterials and tissue engineering approaches are seeking to overcome the limitations associated with these treatment methods. This review critically discusses the advances in biomaterial-based NGCs, their limitations and where future improvements may be required. Recent developments include the incorporation of topographical guidance features and/or intraluminal structures, which attempt to guide Schwann cell (SC) migration and axonal regrowth towards their distal targets. The use of such strategies requires consideration of the size and distribution of these topographical features, as well as a suitable surface for cell-material interactions. Likewise, cellular and molecular-based therapies are being considered for the creation of a more conductive nerve microenvironment. For example, hurdles associated with the short half-lives and low stability of molecular therapies are being surmounted through the use of controlled delivery systems. Similarly, cells (SCs, stem cells and genetically modified cells) are being delivered with biomaterial matrices in attempts to control their dispersion and to facilitate their incorporation within the host regeneration process. Despite recent advances in peripheral nerve repair, there are a number of key factors that need to be considered in order for these new technologies to reach the clinic.

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“…This increase in neurite alignment and growth is accompanied by a significant reduction in the number of neurites and neurite branching. Mahoney et al demonstrated increased alignment and growth of PC12 cells on micro-structured polyimide for features in the range of 20e30 mm versus features which had a width of 40e60 mm [24]. Preliminary work by our own group demonstrated increased aligned growth of PC12 neurites on microstructured PLGA film with features ranging from 5 to 10 mm in width [25].…”

Section: Introductionmentioning

confidence: 98%

The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair

et al. 2012

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Publication InformationDaly, WT,Yao, L,Abu-rub, MT,O'Connell, C,Zeugolis, DI,Windebank, AJ,Pandit, AS (2012) b s t r a c tThe current microsurgical gold standard for repairing long gap nerve injuries is the autograft. Autograft provides a protective environment for repair and a natural internal architecture, which is essential for regeneration. Current clinically approved hollow nerve guidance conduits allow provision of this protective environment; however they fail to provide an essential internal architecture to the regenerating nerve. In the present study both structured and unstructured intraluminal collagen fibres are investigated to assess their ability to enhance conduit mediated nerve repair. This study presents a direct comparison of both structured and unstructured fibres in vivo. The addition of intraluminal guidance structures was shown to significantly decrease axonal dispersion within the conduit and reduced axonal mismatch of distal nerve targets (p < 0.05). The intraluminal fibres were shown to be successfully incorporated into the host regenerative process, acting as a platform for Schwann cell migration and axonal regeneration. Ultimately the fibres were able to provide a platform for nerve regeneration in a long term regeneration study (16 weeks) and facilitated increased guidance of regenerating axons towards their distal nerve targets.

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The influence of microchannels on neurite growth and architecture

Cited by 206 publications

References 28 publications

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair

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