The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro

Nuenen, Marleen H.M.C. van; Ligt, Rianne A.F. de; Doornbos, Robert; Woude, Janneke C. J. Van Der; Kuipers, Ernst J.; Venema, Koen

doi:10.1016/j.femsim.2005.03.010

Cited by 42 publications

(26 citation statements)

References 23 publications

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“…Although these fatty acids have been studied for their role in intestinal motility, 28,29 considerably more studies have focused on butyrate for its role in nourishing the colonic mucosa and in the prevention of colon cancer 30 and also for its influence on cytokine production by immune cells, which has produced so far controversial results. 31,32 L-selectin levels are measured in serum or plasma as a marker of the L-selectin levels in lymphocytes and neutrophils. One early phase of atherosclerosis involves the recruitment of inflammatory cells from the circulation and their transendothelial migration.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effects of a Synbiotic Supplement on Self-Perceived Gastrointestinal Well-Being and Immunoinflammatory Status of Healthy Adults

Nova

Viadel

Wärnberǵ

et al. 2011

Journal of Medicinal Food

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The use of synbiotics as health promoters is still poorly defined, and human intervention studies are scarce. This study was designed to evaluate the effects of a commercialized synbiotic product containing Lactobacillus acidophilus La5, Bifidobacterium animalis ssp. lactis Bb-12, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus paracasei ssp. paracasei, Streptococcus thermophilus, and fructooligosaccharides on the self-reported gastrointestinal well-being and the immunoinflammatory status of healthy human subjects. In this randomized, double-blind, placebo-controlled study, 20 women and 16 men (25-45 years old) received either three tablets per day of the synbiotic product (2.4Â10 9 colony-forming units=day) or placebo during 6 weeks. Gastrointestinal symptoms and bowel habits were evaluated through a self-administered questionnaire. In those subjects suffering from any kind of digestive disturbance (mild dyspepsia, flatulence, postprandial bloating, constipation, etc.), improvements in symptoms after product consumption were also evaluated. Blood lymphocyte subsets, phagocytic activity, serum C-reactive protein, ceruloplasmin, and adhesion molecules concentrations were analyzed prior and after treatment. A significant improvement in overall self-reported gastrointestinal symptoms and bowel habit was found in the synbiotic group. A marginal effect of treatment (analysis of variance P ¼ .050) was observed with L-selectin, which showed a significant decrease in the synbiotic group (P ¼ .019). In addition, basal L-selectin levels correlated with final intercellular adhesion molecule (ICAM)-1 levels (r ¼ 0.468; P ¼ .050), and basal ICAM-1 levels tended to correlate negatively with final L-selectin concentration (r ¼ -0.457; P ¼ .056). None of these correlations was found in the placebo group. The rest of the immunological parameters studied were not modified by the intervention. In conclusion, consumption of the synbiotic product improves self-perceived bowel habits and might facilitate a better profile of adhesion molecules in healthy adults.

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Section: Discussionmentioning

confidence: 99%

Beneficial Effects of a Synbiotic Supplement on Self-Perceived Gastrointestinal Well-Being and Immunoinflammatory Status of Healthy Adults

Nova

Viadel

Wärnberǵ

et al. 2011

Journal of Medicinal Food

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“…Several studies have investigated the trophic effects of butyrate on colonocytes both in vivo and in vitro. In vivo, butyrate plays a critical role in the homeostasis of the colonic epithelium (23)(24)(25). However, in vitro butyrate induces differentiation, inhibits cellular proliferation, and stimulates apoptosis in cultured colorectal cancer cells (26 -28).…”

Section: Effect Of Receptor Depletion On Clonogenic Survivalmentioning

confidence: 99%

Silencing of the Insulin Receptor Isoform A Favors Formation of Type 1 Insulin-Like Growth Factor Receptor (IGF-IR) Homodimers and Enhances Ligand-Induced IGF-IR Activation and Viability of Human Colon Carcinoma Cells

et al. 2010

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Insulin receptor (IR) overexpression is common in cancers, with expression of the A isoform (IR-A, exon 11-) predominating over the B isoform. The IR-A signals a proliferative, antiapoptotic response to IGF-II, which itself can be secreted by tumors to establish an autocrine proliferative loop. Therefore, IGF-II signaling via the IR-A could mediate resistance to type 1 IGF receptor (IGF-IR) inhibitory drugs that are currently in development. This study addressed the role of the IR-A, using a small interfering RNA-based approach in SW480 human colon adenocarcinoma cells that coexpress the IGF-IR. Clonogenic survival was inhibited by depletion of the IGF-IR but not the IR-A, and dual receptor depletion had no greater effect than IGF-IR knockdown alone, suggesting that the IR-A could not compensate for IGF-IR loss. IGF-IR knockdown also resulted in a decrease in viability, whereas IR-A depletion resulted in increased viability. Consistent with this, upon IR-A depletion, we found a concomitant enhancement of IGF-IR activation by IGF-I and IGF-II, reduced formation of IGF-IR:IR-A hybrid receptors and increased IGF-IR homodimer formation. Together, these results suggest that IGF bioactivity is mediated more effectively by the IGF-IR than by the IR-A or receptor hybrids and that signaling via the IGF-IR is dominant to the IR-A in colon cancer cells that express both receptors.

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“…These data indicate that anti‐inflammatory benefits of LKM512 yogurt are influenced by intestinal bacterial metabolites than intestinal bacterial cell components derived from altered large intestinal microbiota. To our knowledge, there is little information supporting the relationship between intestinal bacterial metabolites and host health; however, van Nuenen et al (2005) reported that intestinal microbial metabolites influenced the metabolic integrity of intestinal epithelial cells and mucosal barrier function. However, we do not deny that the recognition of bacterial cell components by TLRs plays a crucial role in host immune system, e.g.…”

Section: Resultsmentioning

confidence: 99%

The influence of the immunostimulation by bacterial cell components derived from altered large intestinal microbiota on probiotic anti-inflammatory benefits

Matsumoto¹,

Hara²,

Benno³

2007

FEMS Immunology &Amp; Medical Microbiology

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Using murine macrophage-like J774.1 cells and fecal precipitates prepared from the feces of elderly volunteers whose acute inflammation had been inhibited by LKM512 yogurt consumption, we investigated the likelihood that immunostimulation by altered intestinal bacterial cell components contribute to the anti-inflammatory benefits of this yogurt. Tumor necrosis factor-alpha production due to stimulation by fecal precipitates obtained during LKM512 yogurt consumption tended to be higher than due to stimulation by precipitates obtained from preconsumption (P=0.0827), although acute phase response was suppressed by LKM512 yogurt consumption. We suggest that the anti-inflammatory benefits of LKM512 yogurt on elderly volunteers are independent of direct immunostimulation by the bacterial cell components derived from altered intestinal microbiota.

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The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro

Cited by 42 publications

References 23 publications

Beneficial Effects of a Synbiotic Supplement on Self-Perceived Gastrointestinal Well-Being and Immunoinflammatory Status of Healthy Adults

Beneficial Effects of a Synbiotic Supplement on Self-Perceived Gastrointestinal Well-Being and Immunoinflammatory Status of Healthy Adults

Silencing of the Insulin Receptor Isoform A Favors Formation of Type 1 Insulin-Like Growth Factor Receptor (IGF-IR) Homodimers and Enhances Ligand-Induced IGF-IR Activation and Viability of Human Colon Carcinoma Cells

The influence of the immunostimulation by bacterial cell components derived from altered large intestinal microbiota on probiotic anti-inflammatory benefits

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