The influence of micro-topography on cellular response and the implications for silicone implants

Recum, Andreas F. von; Kooten, Theo G. van

doi:10.1163/156856295x00698

Cited by 236 publications

(150 citation statements)

References 108 publications

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“…We observed that there is a range of different parameters in the electrospinning process that affect both the scaffold and fibers itself including solvent type, material concentration and viscosity, distance of the collecting target from the spinning nuzzle, the gauge of the needle and voltage, that allow us to obtain uniform fibrous scaffolds. Given that fiber diameter can affect cell attachment, proliferation, migration and cytoskeletal organization, it is likely that "optimal" conditions will need to be determined for each application [37][38][39]. Our results suggest that the gelatin10%/ PCL10% scaffolds may be optimal for cardiovascular tissue engineering applications.…”

Section: Discussionmentioning

confidence: 89%

“…The transmission electron microscope was operated at 100 kV. c) Multiphoton imaging and SHG microscopy-To determine the fiber composition in the electrospun scaffolds, multiphoton imaging and SHG microscopy were performed using a Zeiss LSM 510 META NLO femtosecond laser scanning system (Carl Zeiss MicroImaging Inc., Thornwood, NY, USA), coupled to a software-tunable Coherent Chameleon titanium:sapphire laser (720 nm -930 nm, 90 MHz; Coherent Laser Group, Santa Clara, CA, USA), and equipped with a high-resolution AxioCam HRc camera with 1300 × 1030 pixels (Carl Zeiss) as previously described [36][37][38]. Images were collected using an oil immersion Plan-Neofluar 40×/1.3 numerical aperture (NA) DIC, or an oil immersion Plan Apochromat 63×/1.4 NA objective lens (Carl Zeiss).…”

Section: Ultrastructural Scaffold Analysismentioning

confidence: 99%

“…All observations were made using non-treated samples of collagen10%/elastin5% (not cross-linked, cross-linked, and with PCL10%) and gelatin10% (not cross-linked, cross-linked, and with PCL10%) scaffolds. ECM structure-dependent autofluorescence and SHG were induced using wavelengths of 760 nm (elastin) and 840 nm (collagen) as described previously in more detail [36][37][38]. Non-invasive serial optical horizontal sections of six different areas of each of the specimens were taken in z-steps of 1 μm, 2.5 μm or 5 μm to depths of 50-100 μm.…”

Section: Ultrastructural Scaffold Analysismentioning

confidence: 99%

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Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Section: Discussionmentioning

confidence: 89%

Section: Ultrastructural Scaffold Analysismentioning

confidence: 99%

Section: Ultrastructural Scaffold Analysismentioning

confidence: 99%

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Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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“…By using an electroformed screen mesh mask, various patterns can be transferred from the mask to the surface by taking away the atoms from the unmasked regions. At present, plasma techniques are used preferentially to produce different topologies on surfaces to investigate their cell biological effects [88,89]. Exceptions are microscopic guidance channels for patterned neurite outgrowth [90] and microscopic patterns used to localize cells by electric-field forces [91,92].…”

Section: Surface Morphologymentioning

confidence: 99%

“…These techniques are beyond the scope of this paper since it focuses on techniques with a high potential to induce micro-patterned cell arrangements in in vivo like structures. However, it should be noted that possible correlations between the different contrasting techniques have recently been considered [93,94]. Fig.…”

Section: Surface Morphologymentioning

confidence: 99%

Plasma-surface modification of biomaterials

Chu

Chen

Wang

et al. 2002

Materials Science and Engineering: R: Reports

1,402

652

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Plasma-surface modification (PSM) is an effective and economical surface treatment technique for many materials and of growing interests in biomedical engineering. This article reviews the various common plasma techniques and experimental methods as applied to biomedical materials research, such as plasma sputtering and etching, plasma implantation, plasma deposition, plasma polymerization, laser plasma deposition, plasma spraying, and so on. The unique advantage of plasma modification is that the surface properties and biocompatibility can be enhanced selectively while the bulk attributes of the materials remain unchanged. Existing materials can, thus, be used and needs for new classes of materials may be obviated thereby shortening the time to develop novel and better biomedical devices. Recent work has spurred a number of very interesting applications in the biomedical field. This review article concentrates upon the current status of these techniques, new applications, and achievements pertaining to biomedical materials research. Examples described include hard tissue replacements, blood contacting prostheses, ophthalmic devices, and other products. #

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The effect of a subcutaneous silicone rubber implant with shallow surface microgrooves on the surrounding tissues in rabbits

Braber

Ruijter

Jansen

1997

J. Biomed. Mater. Res.

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It has been suggested that during wound healing microtextured surfaces can alter events at the interface be tween implant surface surface and surrounding tissues. To investigate this phenomenon, smooth and microtextured silicone rubber implants were implanted subcutaneously in rabbits for 3, 7, 42, and 84 days. The textured implants pos sessed parallel surface microgrooves and ridges with a width of 2.0, 5.0, and 10.0 \x.m. All gi'ooves had a depth of approximately 0.5 jxm. SEM observation showed fibroblasts, erythrocytes, lymphocytes, macrophages, fibrin, and colla gen on all implant surfaces after 3 and 7 days. After 42 and 84 days only little collagen, a small number of fibroblasts, but no inflammatory cells were seen on the implant surfaces. The fibroblasts were not oriented along the surface grooves on all textured surfaces. Three-dimensional reconstruction of CLSM images and LM images showed no significant dif ferences between the thickness of the capsules surrounding the smooth and those surrounding the microgrooved im plants. In contrast, LM did show a significantly lower num ber of inflammatory cells and a significantly higher number of blood vessels in the capsules surrounding the micro grooved implants. Differences between the 2,0, 5,0, and 10.0 jxm grooved implants were not detected. Our results con cerning the capsule thickness suggest that the depth of our grooves was not sufficient to facilitate mechanical interlock ing, but the cause for the observed differences in inflamma tory response and number of blood vessels remains unclear. IN T R O D U C T IO N The interaction between an implant material and the surrounding tissues can be considered vital for the final clinical performance of implanted artificial medi cal devices. For example, the promotion of tissue at tachment and the concomitant reduction of the highly undesirable chronic inflammatory response and fibro sis around implant materials are of central importance for the biocompatibility of biomaterials.1 Since various surface properties2 of an implant material determine the biocompatibility of these materials, surface modi fications based on the most recent technologies are being explored in search of the ideal implant surface.3 This study will focus on one of these modifications, i.e. implant surface texturing on a micrometer scale. Earlier studies have shown that microgeometrical

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The influence of micro-topography on cellular response and the implications for silicone implants

Cited by 236 publications

References 108 publications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Plasma-surface modification of biomaterials

The effect of a subcutaneous silicone rubber implant with shallow surface microgrooves on the surrounding tissues in rabbits

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