The influence of maternal smoking on transferrin sialylation and fetal biometric parameters

Wrześniak, Marta; Królik, Małgorzata; Kępińska, Marta; Milnerowicz, Halina

doi:10.1016/j.etap.2016.09.008

Cited by 2 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assessment of cotinine levels confirmed that the level of exposure to tobacco smoke used in this study is equivalent to that of smokers [73][74][75][76] . As for ethanol blood concentrations, they are comparable to those a human fetus would be exposed to after maternal ingestion of a moderate to heavy dose of ethanol 83 .…”

Section: Body Mass Cotinine and Ethanol Levelssupporting

confidence: 73%

“…The smoking machine generates a single 35-ml, 2-s puff per min, containing 89% sidestream smoke (smoke released from the burning end of a cigarette) and 11% mainstream smoke (smoke from the puff stream), as a surrogate for active smoking [49][50][51]71,72 . The pattern of tobacco smoke exposure used in this study 72 generates cotinine (nicotine metabolite) serum levels that are within the range of those found in smokers [73][74][75][76] . Control mice were exposed to ambient air in a chamber identical to the one used for smoke exposure.…”

Section: Animals and Treatmentmentioning

confidence: 67%

See 1 more Smart Citation

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

Nunes‐Freitas

Manhães

Dutra-Tavares

et al. 2020

J Dev Orig Health Dis

View full text Add to dashboard Cite

Either tobacco smoking or alcohol consumption during pregnancy sex-selectively increases susceptibility to drugs of abuse later in life. Considering that pregnant smoking women are frequently intermittent consumers of alcoholic beverages, here, we investigated whether a short-term ethanol exposure restricted to the brain growth spurt period when combined with chronic developmental exposure to tobacco smoke aggravates susceptibility to nicotine in adolescent and adult mice. Swiss male and female mice were exposed to tobacco smoke (SMK; research cigarettes 3R4F, whole-body exposure, 8 h/daily) or ambient air during the gestational period and until the tenth postnatal day (PN). Ethanol (ETOH, 2 g/Kg, 25%, i.p.) or saline was injected in the pups every other day from PN2 to PN10. There were no significant differences in cotinine (nicotine metabolite) and ethanol serum levels among SMK, ETOH and SMK + ETOH groups. During adolescence (PN30) and adulthood (PN90), nicotine (NIC, 0.5 mg/Kg) susceptibility was evaluated in the conditioned place preference and open field tests. NIC impact was more evident in females: SMK, ETOH and SMK + ETOH adolescent females were equally more susceptible to nicotine-induced place preference than control animals. At adulthood, SMK and SMK + ETOH adult females exhibited a nicotine-evoked hyperlocomotor profile in the open field, with a stronger effect in the SMK + ETOH group. Our results indicate that ethanol exposure during the brain growth spurt, when combined to developmental exposure to tobacco smoke, increases nicotine susceptibility with stronger effects in adult females. This result represents a worsened outcome from the early developmental dual exposure and may predispose nicotine use/abuse later in life.

show abstract

Section: Body Mass Cotinine and Ethanol Levelssupporting

confidence: 73%

Section: Animals and Treatmentmentioning

confidence: 67%

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

Nunes‐Freitas

Manhães

Dutra-Tavares

et al. 2020

J Dev Orig Health Dis

View full text Add to dashboard Cite

show abstract

“…30 One possible explanation for our observations would be a modulation of the effect of smoking by pregnancy, although the findings of other recent work are generally inconsistent with this idea. 31 To simplify interpretation of the FS-SRM results, it was decided that statistical analyses should focus on variation that could not be formally explained by differences in protein concentration. Thus, an attempt was made to correct for these differences using simple normalization (eq S1), for which the compositional analysis (see above) and the result in Figure 5A provided some justification.…”

Section: ■ Experimental Proceduresmentioning

confidence: 99%

Refinement of a Methodology for Untargeted Detection of Serum Albumin Adducts in Human Populations

Preston

Plusquin

Sozeri

et al. 2017

Chem. Res. Toxicol.

View full text Add to dashboard Cite

Covalently modified blood proteins (e.g., serum albumin adducts) are increasingly being viewed as potential biomarkers via which the environmental causes of human diseases may be understood. The notion that some (perhaps many) modifications have yet to be discovered has led to the development of untargeted adductomics methods, which attempt to capture entire populations of adducts. One such method is fixed-step selected reaction monitoring (FS-SRM), which analyses distributions of serum albumin adducts via shifts in the mass of a tryptic peptide [Li et al. (2011) Mol. Cell. Proteomics 10, M110.004606]. Working on the basis that FS-SRM might be able to detect biological variation due to environmental factors, we aimed to scale the methodology for use in an epidemiological setting. Development of sample preparation methods led to a batch workflow with increased throughput and provision for quality control. Challenges posed by technical and biological variation were addressed in the processing and interpretation of the data. A pilot study of 20 smokers and 20 never-smokers provided evidence of an effect of smoking on levels of putative serum albumin adducts. Differences between smokers and never-smokers were most apparent in putative adducts with net gains in mass between 105 and 114 Da (relative to unmodified albumin). The findings suggest that our implementation of FS-SRM could be useful for studying other environmental factors with relevance to human health.

show abstract

The influence of maternal smoking on transferrin sialylation and fetal biometric parameters

Cited by 2 publications

References 31 publications

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

Sex- and age-dependent differences in nicotine susceptibility evoked by developmental exposure to tobacco smoke and/or ethanol in mice

Refinement of a Methodology for Untargeted Detection of Serum Albumin Adducts in Human Populations

Contact Info

Product

Resources

About