The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.)

Kojić, Danijela; Popović, Željko D.; Orčić, Dejan; Purać, Jelena; Orčić, Snežana; Vukašinović, Elvira L.; Nikolić, Tatjana; Blagojević, Duško

doi:10.1016/j.jinsphys.2018.07.007

Cited by 23 publications

(34 citation statements)

References 43 publications

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“…The glycogen content was significantly downregulated in overwintering RWW adults, and qPCR results indicated that glycogen synthase was downregulated and glycogen phosphorylase was significantly upregulated. It was reported that fructose-1,6-diphosphatase and pyruvate kinase were inhibited during the glycerol accumulation by activation of glycogen phosphorylase (Kojić et al, 2018). The levels of both these enzymes were decreased (0.82 and 0.81) in overwintering adults, indicating that this route is also closely related to glycerol accumulation.…”

Section: Energy Metabolismmentioning

confidence: 95%

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

et al. 2020

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Adaptations to low temperature play a critical role in restricting the geographical distribution of insects. Decreasing day lengths and temperatures trigger seasonal cold adaptations in insects. These adaptions include changes in expression at the miRNA, mRNA and protein levels. The rice water weevil (RWW), Lissorhoptrus oryzophilus, introduced from the Mississippi River, is a globally invasive pest of wetland rice that can survive at the northern border of China. To investigate the changes in expression at the protein level in overwintering female RWW adults, 6-plex tandem mass tags (TMTs) were used in overwintering and summer adults. By using a proteome database available for Curculionidae, 1077 proteins were quantified, 183 of which differed significantly between the overwintering and summer samples. To further understand these differentially expressed proteins (DEPs), bioinformatics analyses such as gene ontology (GO) enrichment analyses were performed. DEPs associated with the terms binding, structural molecule activity, catalytic activity, multicellular organismal process, extracellular region, chitin binding, metabolic process, intracellular part and organic cyclic compound binding were altered by selection during winter. The changes in the expression of these proteins suggest that the proteins are important for RWW survival in winter.

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Section: Energy Metabolismmentioning

confidence: 95%

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

et al. 2020

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“…When the environmental conditions are not suitable for insects, diapause may be initiated to reduce energy expenses and metabolic activities, and to enhance stress resistance and extend lifespan [10][11][12][13] . Previous studies have comprehensively reviewed diapause from many aspects [14][15][16] , ranging from insects to mammals, and shown that diapause is a complicated process. To date, many studies have focused on ecological characteristics to optimize conditions for diapause induction or termination to improve biological control programs [17,18] .…”

Section: Introductionmentioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Zhang¹,

Du²,

Zhang³

et al. 2020

Preprint

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Background The parasitoid insect Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have focused mostly on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify genes involved in the development of diapause. Results Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. This initially yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3351 bp. Among the differentially expressed genes, 1,204 were specifically expressed during the diapause stage, and 820 were significantly up-regulated. The result of GO enrichment analysis revealed that binding, oxidation−reduction process, and ribosome biogenesis were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on previous studies and our data, several genes were identified to be involved in diapause, including UDP-glucuronosyltransferase; Glutathione-S-transferase, ATGs, zinc finger protein genes, cell cycle-related genes, Trehalase, trehalose transporter, hsp68, p53, and DNA damage-regulated gene 1 (pdrg1), and genes related to lipid metabolism were also included. Conclusions In this study, we generated a great amount of transcriptome data from T. dendrolimi, providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi, but for other species as well.

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“…A complex phenomenon, diapause consists of three major phases -pre-diapause, diapause and post-diapause (quiescence), giving the diapausing organism time to gradually change 1 . Plenty of biochemical and molecular adaptations occur during the different phases of diapause, including: metabolic depression 2 ; reduction in the activity of low-priority metabolic pathways and simultaneous redirection of metabolism towards the synthesis of specific cryoprotective molecules [3][4][5][6][7][8] ; changes in the fatty acid composition of membrane and storage lipids [9][10][11] , and expression of specific sets of genes and proteins which are involved in stress protection and regulation of cell cycle and programmed cell death [12][13][14][15][16] . In addition, prior to diapause entry, an organism is subjected to different behavioural activities such as migration, locating suitable micro-habitats, aggregation, and physiological processes such as accumulation of energy reserves, which altogether lead to increased stress tolerance 1 .…”

mentioning

confidence: 99%

“…), which colligatively decrease the haemolymph's supercooling point while, at the same time, non-colligatively stabilize proteins and cell membranes 3,8,14,[20][21][22][23] . In accordance with that, in the previous GC-MS and 1 H-NMR based metabolomic studies of this species, it was established that the haemolymph of actively developing and diapausing larvae has completely distinct metabolomic profiles 8,24 . Namely, the haemolymph of non-diapausing larvae is enriched in lactate, acetate and succinate, which indicates intensive catabolism to meet the high energy demands for active development.…”

mentioning

confidence: 99%

“…Namely, the haemolymph of non-diapausing larvae is enriched in lactate, acetate and succinate, which indicates intensive catabolism to meet the high energy demands for active development. On the other hand, natural cryoprotectants such as glycerol, proline and alanine, are the predominant metabolites in the haemolymph of diapausing and cold acclimated diapausing larvae 3,8,23,24 .…”

mentioning

confidence: 99%

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Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)

Uzelac

Avramov

Čelić

et al. 2020

Sci Rep

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The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes-citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.

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The influence of low temperature and diapause phase on sugar and polyol content in the European corn borer Ostrinia nubilalis (Hbn.)

Cited by 23 publications

References 43 publications

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)

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