The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis

Abstract

The effects of low medium pH (4.50, 5.00 and 5.75) on in vitro growth and on several biochemical parameters (lipid peroxidation, proline and carbohydrate content, antioxidant enzymes activities and total soluble protein) of Plantago almogravensis and P. algarbiensis micropropagated shoots were investigated. Overall, it was observed that medium pH did not affect in vitro proliferation and rooting. Interestingly, cultures of both species modify the initial pH value to the same final value. Results have shown that the lowest pH tested induced an increase in the level of lipid peroxidation in roots of both species and in shoots of P. algarbiensis, indicating plasma membrane damage. An accumulation of carbohydrates was observed in roots of P. almogravensis cultured in pH 4.50 and 5.00. It was observed a slight response of the enzymatic system to medium pH, particularly in P. almogravensis. Based on the results obtained we can conclude that Plantago species are apt to grow in vitro in medium with pH values much lower than the usually used in tissue culture, which is in agreement with the fact that both species colonize acid soils.

Influence of gibberellin A3 application, pH of the medium, photoperiod and temperature on the enhancement of in vitro flowering in Vitex negundo L.

“…According to Pavlovkin et al (2009), lower pH levels hinder ex vitro flowering as well as growth and development in Lotus corniculatus by having an adverse effect mainly at the root plasmalemma level. This interpretation is also supported by the recent report of Martins et al (2011) who examined pH effects on in vitro growth of Plantago almogravensis and P. algarbiensis.…”

In vitro evaluation of tipburn resistance in lettuce (Lactuca sativa. L)

“…Such an in vitro approach has been developing for understanding environmental stress (Barreto et al 2010;Cui et al 2010;Lokhande et al 2010;Sorkheh et al 2011). The in vitro responses also revealed the effect of some ions (Ghnaya et al 2010;Xu et al 2011) and pH levels (Naik et al 2010;Martins et al 2011) on higher plants. In this study, the assay was applied to calcium deficiency induced with the chelator EGTA.…”

Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

“…This technology is affected by a variety of factors such as pH, light, phytohormone, precursors and elicitor. An accumulation of carbohydrates was observed in the roots of P. almogravensis cultured at pH 4.5 and 5.0 (Neusa et al 2011). A combination of red and blue lights resulted in a greater dry weight and enzyme activity in the tips of proliferating shoots of Oncidium ''Gower Ramsey'' when incubated on 1/2 Murashige and Skoog (MS) medium (Liu et al 2010).…”