The influence of low- and high-molecular chelating additives upon the reduction of nitrotetrazolium blue to formazane in the presence of d-metal cations

DFT and MP2 study of isomery scheme in Formazan and intermolecular and intramolecular proton transfer between its tautomers

“…Preparation of formazan was done by various methods such as reduction of tetrazolium salts and coupling diazonium salts [4,5]. Interestingly, the reduction of nitrotetrazolium blue to formazan is one of the current test methods for the estimation of anti-and pro-oxidant activity of different compounds [6,7]. Another method described is reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide to formazan by the mitochondrial succinate dehydrogenase [4].…”