The Influence of Long-Term Intake of Copper and Chromium Compounds on Reproductive Ability and Development of<i>Drosophila Melanogaster</i>

Živanov-Čurlis, J.; Tomin, J.; Vasiljević, Perica; Vukelić, Mato; Dordević, Lj.; Mitić, Ž.

doi:10.1080/13102818.2006.10817343

Cited by 3 publications

(4 citation statements)

References 21 publications

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“…Copper sulphate (CuSO 4 ), a compound listed as a pesticide, is toxic to flies (Živanov-Čurlis et al , 2006; Alaraby, Hernández and Marcos, 2017; Balinski and Woodruff, 2017; Halmenschelager and da Rocha, 2019), and our unpublished data suggests that flies avoid it. Therefore, we decided to condition ~ 24 hour starved flies both in T-maze tubes and in training vials with 80 mM CuSO4 along with 200 mM arabinose, a sweet but not metabolisable sugar, as a carrier to promote ingestion of CuSO4.…”

Section: Resultsmentioning

confidence: 98%

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Enhanced olfactory memory performance in trap-design Y-mazes allows the study of novel memory phenotypes inDrosophila

Mohandasan

Thakare

et al. 2020

Preprint

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The neural basis of behaviour is identified by systematically disrupting the activity of specific neurons and screening for loss in phenotype. High scoring behavioural assays are thus necessary for identifying the neural circuits of novel behaviours. Here, we report the design and use of a Y-maze based classical olfactory learning and memory assay in Drosophila. Appetitive memory scores in our Y-maze are considerably greater and longer-lasting than that from a commonly used T-maze design. We show that testing in the Y-maze is key to the improved scores. We also observed considerable 24-hour aversive taste reinforced memory performance with only one training trial using Y-mazes. This allowed us to determine the protein synthesis dependence of long-lasting aversive taste memories for the first time in flies. The Y-maze assembly will make olfactory conditioning more accessible and it will allow the study of novel memory phenotypes in Drosophila.

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Section: Resultsmentioning

confidence: 98%

“…Copper sulphate (CuSO 4 ), a compound listed as a pesticide, is toxic to flies (Živanov-Čurlis et al ., 2006; Alaraby, Hernández and Marcos, 2017; Balinski and Woodruff, 2017; Halmenschelager and da Rocha, 2019). Our unpublished data shows that flies avoid it.…”

Section: Resultsmentioning

confidence: 99%

Enhanced olfactory memory performance in trap-design Y-mazes allows the study of novel memory phenotypes inDrosophila

Mohandasan

Thakare

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Copper sulfate (CuSO 4 ), a compound listed as a pesticide, is toxic to flies ( Živanov-Čurlis et al 2006 ; Balinski and Woodruff 2017 ; Halmenschelager and da Rocha 2019 ). Our unpublished data suggested that flies avoid CuSO 4 .…”

Section: Resultsmentioning

confidence: 99%

Enhanced olfactory memory detection in trap-design Y-mazes allows the study of imperceptible memory traces in Drosophila

et al. 2022

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The neural basis of behavior is identified by systematically manipulating the activity of specific neurons and screening for loss or gain of phenotype. Therefore, robust, high-scoring behavioral assays are necessary for determining the neural circuits of novel behaviors. We report a simple Y-maze design for Drosophila olfactory learning and memory assay. Memory scores in our Y-mazes are considerably better and longer-lasting than scores obtained with commonly used T-mazes. Our results suggest that trapping flies to an odor choice in a Y-maze could improve scores. We postulated that the improved scores could reveal previously undetectable memory traces, enabling the study of underlying neural mechanisms. Indeed, we identified unreported protein synthesis-dependent long-term memories (LTMs), reinforced by ingestion of (1) an aversive compound and (2) a sweet but nonnutritious sugar, both 24 h after training. We also used Y-mazes to probe how using a greater reward may change memory dynamics. Our findings predict that a greater sugar reward may extend existing memory traces or reinforce additional novel ones.

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“…Toxicity investigations of various commercial copper salts show a wide range of values for LD 50 . The level of the acute toxicity is higher for more soluble than for less soluble Cu(II) salts [26]. The results of our pharmacological investigations point to the lower toxicity of Cu(II) complex with RLMD, what is much better than in the case of commercially applicable inorganic copper salts [2].…”

Section: Resultsmentioning

confidence: 99%

The investigations of bioactive copper(II) complexes with reduced low-molar dextran

et al. 2008

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The optimization of the reaction conditions (pH,T,t) to obtain stable copper(II) ion complexes with dextran derivatives were investigated in this paper. A complete synthesis of stable aqueous complexes can be realized with reduced low-molar polysaccharides, at an average molar mass 5000 g mol–1and pH 7.5–8. Fourier-transform IR spectra of polysaccharide dextran and its compounds with copper(II) ion, recorded at room temperature, were analyzed in order to obtain the information about the structure and the conformation of these polymer compounds. The ESR parameters of the spectra indicate the square-planar coordination of Cu(II) ion with four O atoms. Copper(II) complex formation with dextran and its derivatives were analyzed by physicochemical methods. Synthesized complexes of Cu(II) ion with reduced low-molar dextran in comparison with commercial preparations showed the considerably lower acute toxicity (LD501705).

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The Influence of Long-Term Intake of Copper and Chromium Compounds on Reproductive Ability and Development ofDrosophila Melanogaster

Cited by 3 publications

References 21 publications

Enhanced olfactory memory performance in trap-design Y-mazes allows the study of novel memory phenotypes inDrosophila

Enhanced olfactory memory performance in trap-design Y-mazes allows the study of novel memory phenotypes inDrosophila

Enhanced olfactory memory detection in trap-design Y-mazes allows the study of imperceptible memory traces in Drosophila

The investigations of bioactive copper(II) complexes with reduced low-molar dextran

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