The influence of local pressure on evaluation parameters of skin blood perfusion and fluorescence

Zherebtsov, Evgeny; Kandurova, Ksenia; Seryogina, Evgeniya; Kozlov, Igor; Dremin, Viktor; Zherebtsova, Angelina I.; Dunaev, Andrey V.; Meglinski, Igor

doi:10.1117/12.2267957

Cited by 16 publications

(16 citation statements)

References 14 publications

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“…The measurements were carried out by the laser Doppler flowmeter LAKK-02 (SPE LAZMA Ltd., Russia) with laser probing radiation of 1064 nm. The calibrated pressure was applied to skin by use of an in-house designed tool (1 in the Fig.1), [8,6] which allowed one to distribute the weight of placed leads (4) over the LDF probe (2) installed into the parallel hole (3). During the experiment the probe tip (5) was the only area of contact with skin.…”

Section: Protocol Designmentioning

confidence: 99%

“…In previous studies on this topic, most spectroscopic measurements have been conducted by means of a topical optical fiber probe that transmits and collects optical spectra from the skin. Probe pressure influences the microcirculation parameters which can be measured by optical methods: diffuse reflectance spectroscopy [4,5], fluorescence spectroscopy [4,6,7] and Laser Doppler flowmetry (LDF) [8,9]. LDF [10] together with wavelet analyses [11] is a commonly used technique to study the regulatory mechanisms of cutaneous microcirculation.…”

Section: Introductionmentioning

confidence: 99%

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Optical probe pressure effects on cutaneous blood flow

Mizeva

Potapova

Dremin

et al. 2019

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The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure induces vasodilation, whereas the increased pressure is able to stop the blood flow in the compressed area, as well as to significantly change optical signals.The blood flow oscillations measured by laser Doppler flowmetry (LDF) characterize the functional state of the microvascular system and can be used for noninvasive diagnostics of its abnormality. This study was intended to identify the patterns of the relationship between the oscillating components of blood flow registered by the LDF method under different levels of pressure applied to an optical fiber probe.For this purpose we have developed an original optical probe capable of regulating the applied pressure. The developed protocol included six sequential records of the blood perfusion at pressure within the 0 to 200 mmHg range with unloading at the last stage.Using wavelet analyses, we traced the variation of energy of oscillations for these records in five frequency bands associated with different vascular tone regulation mechanisms. Six young volunteers of the same age (three males and three females) were included in this preliminary study and the protocol was repeated five times in each volunteer. Totally 30 LDF records were available for the analyses. As expected, the LDF signal increases at weak pressure (30 mmHg) and decreases at increased pressure. The statistically stable amplification of endothelial associated blood flow oscillations under the 90 mmHg pressure allowed us to put forward a hypothesis that the endothelial activity increases. The possible causes of this phenomenon are discussed.

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Section: Protocol Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical probe pressure effects on cutaneous blood flow

Mizeva

Potapova

Dremin

et al. 2019

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“…9 The amplitude value growth during stages 4 and 5 at low-frequency sub-bands is associated with the fact that more blood vessels become involved only in cardiac oscillations because of high pressure influence on other oscillations. 10…”

Section: Methodsmentioning

confidence: 99%

Analysis of changes in blood flow oscillations under different probe pressure using laser Doppler spectrum decomposition

Kozlov¹,

Zherebtsov²,

Mezentsev³

et al. 2019

Medical Laser Applications and Laser-Tissue Interactions IX

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Presently, in the modern laser Doppler flowmetry (LDF) the distribution of blood perfusion and its changes along the Doppler shift frequencies are simply ignored and/or not properly addressed. Utilizing the registered power spectrum of photocurrent, we introduce an LDF signal processing approach suitable for expanding of diagnostic capabilities of the technique. In particular, we demonstrate that it is possible to determine how the oscillations of blood flow (cardiac, breathe, myogenic, etc.) are distributed along the Doppler shift frequency. Wavelet analysis is utilized to extract the oscillations corresponded to the particular frequency sub-bands of blood perfusion. The main purpose of this study is to identify influence of local pressure by fiber optic probe on cardiac oscillations and their distribution along frequency of Doppler shift.

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“…The fluorescence spectroscopy system with fibre-optical probe "LAKK-M" (SPE "LAZMA" Ltd, Russia) was used for the in vivo measurements 13,14 . The system provides multiwavelength excitation, detects emission, and processes the fluorescence signal.…”

Section: The Methods Of Researchmentioning

confidence: 99%

Noninvasive control of rhodamine-loaded capsules distribution in vivo

Stelmashchuk

Tarakanchikova

Seryogina³

et al. 2018

Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX

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Using fluorescence spectroscopy system with the fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control group, and the group received injections of the nanocapsules. The fluorescent measurements conducted transcutaneously on the thigh surface. The administration of the preparation with the rhodamine concentration of 5 mg/kg of animal weight resulted in a twofold increase of fluorescence intensity by reference to the baseline level. As a result of the study, it was concluded that fluorescence spectroscopy can be used for transdermal measurements of the rhodamine-loaded capsules in vivo.Keywords: nanocomposite polymeric capsules, fluorescent labelled particles, rhodamine TRITC, drug delivery, fluorescence spectroscopy, blood microcirculation, optical measurements in vivo 1. * o.a.stelmashuk@gmail.com; phone +7 919 269 79 92; www.bmecenter.ru/en/fluorescence_spectroscopy_in_vivo

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The influence of local pressure on evaluation parameters of skin blood perfusion and fluorescence

Cited by 16 publications

References 14 publications

Optical probe pressure effects on cutaneous blood flow

Optical probe pressure effects on cutaneous blood flow

Analysis of changes in blood flow oscillations under different probe pressure using laser Doppler spectrum decomposition

Noninvasive control of rhodamine-loaded capsules distribution in vivo

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