The influence of lipid composition on the barrier properties of band 3-containing lipid vesicles

Hoogevest, Peter van; Maine, A.P.M. Du; Kruijff, Ben de; Gier, J. De

doi:10.1016/0005-2736(84)90426-7

Cited by 19 publications

(5 citation statements)

References 42 publications

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“…1). Similar discrepancies between the average vesicle diameters obtained from electron microscopy and from vi-measurements, respectively, were also observed in other studies on band 3 reconstitution [20,27,36].…”

Section: Intravesicular Volume and Transport Numberssupporting

confidence: 83%

“…Four main vesicle populations can be expected to occur ( Fig. 1): (1) sealed vesicles containing the reconstituted anion transport system; (2) sealed vesicles which only show protein-or detergentinduced anion leak flux [22,36]; (3) sealed vesicles containing neither of the transport pathways of populations 1 and 2; and (4) unsealed vesicles, i.e., vesicles which are so leaky that all the intravesicular radioactivity is lost before the beginning of the flux measurements. The vesicles of population 1 may or may not show leak flux and will thus consist of two subpopulations.…”

Section: General Considerationsmentioning

confidence: 99%

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A new method for the reconstitution of the anion transport system of the human erythrocyte membrane

et al. 1986

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The anion transport protein of the human erythrocyte membrane, band 3, was solubilized and purified in solutions of the non-ionic detergent Triton X-100. It was incorporated into spherical lipid bilayers by the following procedure: Dry phosphatidylcholine was suspended in the protein solution. Octylglucopyranoside was added until the milky suspension became clear. The sample was dialyzed overnight against detergent-free buffer. Residual Triton X-100 was removed from the opalescent vesicle suspension by sucrose density gradient centrifugation and subsequent dialysis. Sulfate efflux from the vesicles was studied, under exchange conditions, using a filtration method. Three vesicle subpopulations could be distinguished by analyzing the time course of the efflux. One was nearly impermeable to sulfate, and efflux from another was due to leaks. The largest subpopulation, however, showed transport characteristics very similar to those of the anion transport system of the intact erythrocyte membrane: transport numbers (at 30 degrees C) close to 20 sulfate molecules per band 3 and min, an activation energy of approx. 140 kJ/mol, a pH maximum at pH 6.2, saturation of the sulfate flux at sulfate concentrations around 100 mM, inhibition of the flux by H2DIDS and flufenamate (approx. KI-values at 30 degrees C: 0.1 and 0.7 microM, respectively), and "right-side-out" orientation of the transport protein (as judged from the inhibition of sulfate efflux by up to 98% by externally added H2DIDS). Thus, the system represents, for the first time, a reconstitution of all the major properties of the sulfate transport across the erythrocyte membrane.

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Section: Intravesicular Volume and Transport Numberssupporting

confidence: 83%

Section: General Considerationsmentioning

confidence: 99%

A new method for the reconstitution of the anion transport system of the human erythrocyte membrane

et al. 1986

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“…Partially purified erythrocyte band 3 protein reconstituted into PC vesicles has a similar ability of inducing lipid translocation 195 and enhancing permeability which is significantly diminished in the presence of erythrocyte lipids. 196 Likewise, the incorporation of intrinsic mitochondrial membrane proteins into synthetic vesicles also affects flip rates. For example, external addition of cytochrome b 5 to asymmetric PC/PE (9:1) SUVs resulted in an initial burst of PE flop as detected by the TNBS assay.…”

Section: B Reconstitution Of Membrane Proteinsmentioning

confidence: 99%

Chemical control of phospholipid distribution across bilayer membranes

Boon

Smith

2002

Medicinal Research Reviews

213

172

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Most biological membranes possess an asymmetric transbilayer distribution of phospholipids. Endogenous enzymes expend energy to maintain the arrangement by promoting the rate of phospholipid translocation, or flip-flop. Researchers have discovered ways to modify this distribution through the use of chemicals. This review presents a critical analysis of the phospholipid asymmetry data in the literature followed by a brief overview of the maintenance and physiological consequences of phospholipid asymmetry, and finishes with a list of chemical ways to alter phospholipid distribution by enhancement of flip-flop.

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“…The former case includes (1) membrane proteins, e.g., glycophorin, band 3, and cytochrome b 5 Gerritsen et al, 1980;Greenhut & Roseman, 1985), and (2) channel (pore) formers, e.g., gramicidin, amphotericin B, and bacterial cytotoxins (Classen et al, 1987;Schneider et al, 1986). In some cases, enhanced membrane permeability was also observed at the same time (Bramhall et al, 1987;Classen et al, 1987;Schneider et al, 1986;Van Hoogevest et al, 1984). However, the solute permeation is usually much faster (seconds to minutes) than the enhanced lipid movement (hours).…”

Section: % Flip-flopmentioning

confidence: 99%

An Antimicrobial Peptide, Magainin 2, Induced Rapid Flip-Flop of Phospholipids Coupled with Pore Formation and Peptide Translocation

et al. 1996

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The effect of an antimicrobial peptide, magainin 2, on the flip-flop rates of phospholipids was investigated by use of fluorescent lipids, i.e., anionic N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L-alpha- phosphatidylethanolamine (NBD-PE), 1-oleoyl-2-[12-((7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)- dodecanoyl]-L-alpha-phosphatidic acid (C12-NBD-PA), 1-oleoyl-2-[12- ((7-nitrobenz-2-oxa-1,3-diazol-4-yl)- amino)dodecanoyl]-L-alpha-phosphatidyl-L-serine (C12-NBD-PS), and zwitterionic 1-palmitoyl-2-[6-((7- nitrobenz-2-oxa-1,3-diazol-4-yl)amino)caproyl]-L-alpha-phosphatidy lcholine (C6-NBD-PC). Their intrinsic flip-flop half-lives at 30 degrees C in the absence of the peptide were 1.1 h, ca. 7 h, ca. 8 days, and > 2 days, respectively. The peptide accelerated the flip-flop half-lives of the fluorescent lipids to an order of minutes. Furthermore, the flip-flop was coupled with the membrane permeabilization and the peptide translocation [Matsuzaki, K., Murase, O., Fujii, N., & Miyajima, K. (1995) Biochemistry 34, 6521-6526], suggesting pore-mediated flip-flop. The flip-flop rate was independent of the initial labeling conditions (outer leaflet label or inner leaflet label). From these results, a model was proposed, in which the lipids translocate across the membrane by lateral diffusion along the wall of the pores composed of the peptides and the lipids. A simple theoretical calculation could explain the coupling of the flip-flop with the permeabilization.

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The influence of lipid composition on the barrier properties of band 3-containing lipid vesicles

Cited by 19 publications

References 42 publications

A new method for the reconstitution of the anion transport system of the human erythrocyte membrane

A new method for the reconstitution of the anion transport system of the human erythrocyte membrane

Chemical control of phospholipid distribution across bilayer membranes

An Antimicrobial Peptide, Magainin 2, Induced Rapid Flip-Flop of Phospholipids Coupled with Pore Formation and Peptide Translocation

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