The influence of lead and arsenite on the inhibition of human breast cancer MCF-7 cell proliferation by American ginseng root (Panax quinquefolius L.)

Corbit, Rebecca; Ebbs, Stephen D.; King, Mandy L.; Murphy, Laura

doi:10.1016/j.lfs.2005.07.010

Cited by 28 publications

(18 citation statements)

References 39 publications

(38 reference statements)

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“…This cancer is the second-leading cause of cancer-related deaths and the second most prevalent cancer worldwide (7,18). Although colon tumorigenesis may be one of the best-understood human solid tumors, the clinical management of advanced colon cancer remains highly challenging.…”

Section: Discussionmentioning

confidence: 99%

“…Chen] (3), two important species in genus Panax, whereby ginsenosides Rg3 and Rh2 have been recognized as active anti-cancer compounds (4). Like Asian ginseng (2,5), American ginseng also has anti-cancer activities (6,7). In addition, American ginseng extract was found to enhance the anti-cancer effect of chemotherapeutic agents and attenuate cisplatin-induced nausea and vomiting (8,9).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells

Luο¹,

Wang²,

Jin³

et al. 2008

Int J Oncol

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American ginseng (Panax quinquefolius L., Araliaceae) possesses anti-cancer potential and is one of the most commonly used herbal medicines in the United States. Ginsenoside Rg3, one of the saponins in American ginseng, has been shown to inhibit tumor growth. In this study, we sought to characterize the downstream genes targeted by American ginseng extracts in HCT-116 human colorectal cancer cells. We first demonstrated that the content of Rg3 in American ginseng steamed at 120°C for 2 h (referred to as S2h) was significantly increased when compared with that of the unsteamed ginseng. Both S2h and Rg3 exhibited antiproliferative effects on HCT-116 cells. Using the Affymetrix high density genechips containing more than 40,000 genes and ESTs, the gene expression profiling of HCT-116 cells were assayed. Microarray data indicated that the expression levels of 76 genes were changed significantly after treatment with S2h or Rg3, whereby it was found that 52 of the 76 genes were up-regulated while the remaining 24 were down-regulated. Ingenuity Pathways Analysis of top functions affected by both S2h and Rg3 were carried out. The most effected pathway is the Ephrin receptor pathway. To validate the microarray data, quantitative real-time PCR of six candidate target genes was conducted, whereby it was found that three genes were up-regulated (AKAPA8L, PMPCB and PDE5A) and three were down-regulated (PITPNA, DUS2L and RIC8A). Although further studies are needed to elucidate the mechanisms of action, our findings should expand the understanding of the molecular framework of American ginseng as an anti-cancer agent.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells

Luο¹,

Wang²,

Jin³

et al. 2008

Int J Oncol

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“…Support for low-dose effects comes from studies reporting diminished proliferation/viability of rodent cells at concentrations as low as 0.03 lM (6 lg/L) MeHg and 0.5-1 lM (100-200 lg/L) Pb, which was primarily applied as PbAc (Chen et al 2003;Sakaue et al 2005;Tamm et al 2006;Li et al 2007). Numerous other studies demonstrated that cell proliferation and/or cell viability becomes increasingly reduced at about fivefold to tenfold higher concentration and beyond (e.g., Gribble et al 2005;Corbit et al 2006;Glahn et al 2008;Agarwal et al 2009). …”

Section: Effects Of Mehg On Cell Number and Cell Size Of Afs Cellsmentioning

confidence: 99%

Proliferation potential of human amniotic fluid stem cells differently responds to mercury and lead exposure

et al. 2011

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There are considerable gaps in our knowledge on cell biological effects induced by the heavy metals mercury (Hg) and lead (Pb). In the present study we aimed to explore the effects of these toxicants on proliferation and cell size of primary human amniotic fluid stem (AFS) cells. Monoclonal human AFS cells were incubated with three dosages of Hg and Pb (single and combined treatment; ranging from physiological to cytotoxic concentrations) and the intracellular Hg and Pb concentrations were analyzed, respectively. At different days of incubation the effects of Hg and Pb on proliferation, cell size, apoptosis, and expression of cyclins and the cyclin-dependent kinase inhibitor p27 were investigated. Whereas we found Hg to trigger pronounced effects on proliferation of human AFS cells already at low concentrations, anti-proliferative effects of Pb could only be detected at high concentrations. Exposure to high dose of Hg induced pronounced downregulation of cyclin A confirming the anti-proliferative effects observed for Hg. Co-exposure to Hg and Pb did not cause additive effects on proliferation and size of AFS cells, and on cyclin A expression. Our here presented data provide evidence that the different toxicological effects of Pb and Hg on primary human stem cells are due to different intracellular accumulation levels of these two toxicants. These findings allow new insights into the functional consequences of Pb and Hg for mammalian stem cells and into the cell biological behavior of AFS cells in response to toxicants.

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“…American ginseng extracts were found to inhibit the growth of breast cancer cells (Corbit et al, 2006). We previously investigated the effects of several herbal extracts on reducing chemotherapeutic side-effects and found that American ginseng can attenuate cisplatininduced nausea and vomiting in a rat model, while not affecting its anti-cancer properties in human cancer cells (Mehendale et al, 2005;Aung et al, 2007).…”

Section: American Ginsengmentioning

confidence: 99%

Potential Role of Ginseng in the Treatment of Colorectal Cancer

2008

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Colorectal cancer remains one of the most prevalent cancer and a leading cause of cancer related death in the US. Many currently used chemotherapeutic agents are derived from botanicals. Identifying herbal sources, including those from ginseng family, to develop better anti-cancer therapies remains an essential step in advancing the treatment of the cancer. In this article, potential roles of ginseng herbs, especially American ginseng and notoginseng, in colorectal cancer therapeutics are presented. The major pharmacologically active constituents of ginsengs are ginsenosides, which can be mainly classified as protopanaxadiol and protopanaxatriol groups. Structure-activity relationship between their chemical structures and pharmacological activities are discussed. In addition, various steaming temperature and time treatment of the ginseng herbs can change ginsenoside profiles, and enhance their anti-cancer activities. This heat treatment process may increase the role of ginseng in treating colorectal cancer.

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The influence of lead and arsenite on the inhibition of human breast cancer MCF-7 cell proliferation by American ginseng root (Panax quinquefolius L.)

Cited by 28 publications

References 39 publications

Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells

Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells

Proliferation potential of human amniotic fluid stem cells differently responds to mercury and lead exposure

Potential Role of Ginseng in the Treatment of Colorectal Cancer

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