The influence of irrigation solutions in the inorganic and organic radicular dentine composition

Barón, Marta Serrat; Morales, Victoria; Fuentes, Ma Victoria; Linares, María; Escribano, Nadia Picazo; Ceballos, Laura

doi:10.1111/aej.12395

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…Moreover, bacterial acids (lactic acid), dietary acids (acetic acid, phosphoric acid, and citric acid), and gastric acid {hydrochloric acid (HCl)}, all demineralized the dentin through the processes of caries and acid-erosion. It has become standard to use laboratory acids, such as formic acid [ 12 , acetic and lactic acids [ 13 ], or chelating agents such as EDTA [ 14 ], to produce demineralized dentin models for use in in vitro remineralization studies. It is well known that each demineralizing agent has a unique effect as chelating agents, strong acids, and weak acids affecting both mineral and organic phases of dentin in significantly different ways.…”

Section: Introductionmentioning

confidence: 99%

Demineralized Dentin Matrix for Dental and Alveolar Bone Tissues Regeneration: An Innovative Scope Review

Grawish

et al. 2022

Tissue Eng Regen Med

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Background: Dentin is a permeable tubular composite and complex structure, and in weight, it is composed of 20% organic matrix, 10% water, and 70% hydroxyapatite crystalline matrix. Demineralization of dentin with gradient concentrations of ethylene diamine tetraacetic acid, 0.6 N hydrochloric acid, or 2% nitric acid removes a major part of the crystalline apatite and maintains a majority of collagen type I and non-collagenous proteins, which creates an osteoinductive scaffold containing numerous matrix elements and growth factors. Therefore, demineralized dentin should be considered as an excellent naturally-derived bioactive material to enhance dental and alveolar bone tissues regeneration. Method: The PubMed and Midline databases were searched in October 2021 for the relevant articles on treated dentin matrix (TDM)/demineralized dentin matrix (DDM) and their potential roles in tissue regeneration. Results: Several studies with different study designs evaluating the effect of TDM/DDM on dental and bone tissues regeneration were found. TDM/DDM was obtained from human or animal sources and processed in different forms (particles, liquid extract, hydrogel, and paste) and different shapes (sheets, slices, disc-shaped, root-shaped, and barrier membranes), with variable sizes measured in micrometers or millimeters, demineralized with different protocols regarding the concentration of demineralizing agents and exposure time, and then sterilized and preserved with different techniques. In the act of biomimetic acellular material, TDM/DDM was used for the regeneration of the dentin-pulp complex through direct pulp capping technique, and it was found to possess the ability to activate the odontogenic differentiation of stem cells resident in the pulp tissues and induce reparative dentin formation. TDM/DDM was also considered for alveolar ridge and maxillary sinus floor augmentations, socket preservation, furcation perforation repair, guided bone, and bioroot regenerations as well as bone and cartilage healing. Conclusion: To our knowledge, there are no standard procedures to adopt a specific form for a specific purpose; therefore, future studies are required to come up with a well-characterized TDM/DDM for each specific application. Likely as decellularized dermal matrix and prospectively, if the TDM/DDM is supplied in proper consistency, forms, and in different sizes with good biological properties, it can be used efficiently instead of some widely-used regenerative biomaterials.

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Section: Introductionmentioning

confidence: 99%

Demineralized Dentin Matrix for Dental and Alveolar Bone Tissues Regeneration: An Innovative Scope Review

Grawish

et al. 2022

Tissue Eng Regen Med

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“…3 c has a strong positive weighted peak around 3430 cm −1 , which is related to O–H or N–H stretching vibration. The negatively weighted peak at 1245 cm −1 , was due to amide III, and the negative weighted peaks around 1132 and 960 cm −1 were related to the PO 4 3− stretching vibration 31 , 32 . Therefore, the loadings plot of PC 2 is related to the changes of PO 4 3− and proteins in roots of M. oleifera .…”

Section: Resultsmentioning

confidence: 98%

Soil-applied selenite increases selenium and reduces cadmium in roots of Moringa oleifera

Liu

et al. 2020

Sci Rep

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Deficiency of selenium (Se) will lead to malnutrition and decreased immune function of the body. There is a common phenomenon of Se deficiency in foods. In this study, different concentrations of sodium selenite (Na2SeO3) were applied to Moringa oleifera grownin soil. The purpose was to explore the feasibility of Se biofortification of M. oleifera root. The effect of exogenous Se on the accumulation of Se and cadmium (Cd) in the roots of M. oleifera was studied by inductively coupled plasma mass spectrometry, and the mechanism of exogenous Se on the accumulation of Se and Cd in the roots was studied by Fourier transform infrared spectroscopy (FTIR) combined with principal component analysis and partial least squares regression analysis. The results showed that Na2SeO3 significantly affected the accumulation of Se and Cd in the roots (p < 0.05). The increase in Se was highest when Na2SeO3 was around 4.0 mg/kg, which increased by 315% compared with the control. The decrease in Cd was the lowest when Na2SeO3 was around 2.0 mg/kg, which decreased by 80% compared with the control. The results of FTIR analysis showed that Na2SeO3 treatment changed the carboxylate, phosphate radical, hemicellulose and protein in roots of M. oleifera, while the increase of Se was related to hemicellulose, protein, polysaccharide and lignin, and the decrease of Cd was related to hemicellulose and protein. The results showed that exogenous Se increased the accumulation of Se and inhibited the absorption of Cd. Therefore, the roots of M. oleifera can be used in Se biofortified products.

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“…According to the FTIR analysis, Amide III/

{PO}_{4}^{3 -}

ratio tended to be lower in the groups using NaOCl in the final irrigation step than the control group. This is probably related to the organic tissue dissolving ability of NaOCl (Barón et al., 2020). Similarly, previous studies reported that Amide III/

{PO}_{4}^{3 -}

ratio in dentine decreased significantly after NaOCl application (Morgan et al., 2019; Tartari et al., 2016, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effect of final irrigation protocols with chitosan nanoparticle and genipin on dentine against collagenase degradation: An ex‐vivo study

Şengül,

Ozturk,

Ulubayram

et al. 2024

Int Endodontic J

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AimEndodontic irrigants may affect the mechanical and chemical properties of dentine. This study evaluated the effects of various final irrigation protocols including the use of chitosan nanoparticle (CSnp) and cross‐linking with genipin on the (1) mechanical and (2) chemical properties of dentine against enzymatic degradation.MethodologyCSnp was synthesized and characterized considering physiochemical parameters and stability. The root canals of 90 single‐rooted teeth were prepared and irrigated with NaOCl. Dentine discs were obtained and divided into groups according to the following irrigation protocols: Group NaOCl+EDTA, Group NaOCl+CSnp, Group NaOCl+EDTA+CSnp, Group NaOCl+CSnp+Genipin, Group NaOCl+EDTA+CSnp+Genipin and Group distilled water. (1) Mechanical changes were determined by microhardness analysis using Vickers‐tester. (2) Chemical changes were determined by evaluating molecular and elemental compositions of dentine using Fourier transform infrared spectroscopy (FTIR) analysis and scanning electron microscope (SEM)/energy dispersive X‐ray spectroscopy (EDS) analysis, respectively. All analyses were repeated after the discs were kept in collagenase for 24 h. Data were analysed with repeated measures analysis of variance and Bonferroni correction for microhardness analysis, and Kruskal–Wallis and Wilcoxon tests for FTIR and SEM/EDS analyses (p = .05).Results(1) Collagenase application did not have a negative effect on microhardness only in Group NaOCl+EDTA+CSnp+Genipin when compared with the post‐irrigation values (p > .05). Post‐collagenase microhardness of Group NaOCl+EDTA+CSnp and Group NaOCl+CSnp+Genipin was similar to the initial microhardness (p > .05). (2) After collagenase, Amide III/ ratio presented no change in Group NaOCl+EDTA+CSnp, Group NaOCl+CSnp+Genipin and Group NaOCl+EDTA+CSnp+Genipin (p > .05), while decreased in other groups (p < .05). Collagenase did not affect / ratio in the groups (p > .05). There were no changes in the groups in terms of elemental level before and after collagenase application (p > .05).ConclusionsCSnp and genipin positively affected the microhardness and molecular composition of dentine. This effect was more pronounced when CSnp was used after EDTA.

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The influence of irrigation solutions in the inorganic and organic radicular dentine composition

Cited by 6 publications

References 30 publications

Demineralized Dentin Matrix for Dental and Alveolar Bone Tissues Regeneration: An Innovative Scope Review

Demineralized Dentin Matrix for Dental and Alveolar Bone Tissues Regeneration: An Innovative Scope Review

Soil-applied selenite increases selenium and reduces cadmium in roots of Moringa oleifera

Effect of final irrigation protocols with chitosan nanoparticle and genipin on dentine against collagenase degradation: An ex‐vivo study

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