The quantal content of the e.p.p. and the frequency of the m.e.p.p.s in the presence of enhanced extracellular K+ have been investigated by intracellular recording from frog sartorius muscle fibres. In the presence of 0-23 mM Ca2+ (one eighth of the normal), the replacement of NaCl by sucrose or glycine led to a large increase in quantal content; the size of this increase appeared to be related to the amount of NaCl replaced. The quantal content in 09 mM Ca2+, however, was not appreciably altered by the replacement of NaCl by sucrose. When LiCl was used to replace NaCl, the immediate change in the quantal content was small. With methyl-and ethylammonium or tris chlorides as substitutes for NaCl, the immediate effect was a reduction in quantal content. A delayed effect of LiCl was a reduction, and that of methylammonium an increase, in quantal content. The replacement of NaCl by sucrose accelerated the m.e.p.p. frequency in the presence of enhanced K+ (12 mM); in contrast the replacement of NaCl by methylammonium chloride caused a reduction in frequency. With LiCl as a substitute the frequency of the m.e.p.p.s increased in the normal K+ concentration, the increase being slowly reversible in the normal Ringer solution.It is suggested that antagonism of Ca2+ by Na+ is non-specific and that other monovalent cations will also compete with Ca2 .