The influence of ionic strength on the interaction of viruses with charged surfaces under environmental conditions

Schaldach, Charlene M.; Bourcier, William L.; Shaw, Henry F.; Viani, B.E.; Wilson, W. D.

doi:10.1016/j.jcis.2005.06.082

Cited by 53 publications

(42 citation statements)

References 29 publications

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“…For MS2 phage, the measured IEP value (ϳ3.5 to 3.9) is lower than that expected if considering the capsid (IEP of Ͼ8) (10,15,19) only. As detailed previously (10), the dependence of electrophoretic mobility of MS2 phage on solution ionic strength is typical of that classically encountered for soft (i.e., permeable) particles.…”

contrasting

confidence: 59%

Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles

Dika

Duval

Ly-Chatain

et al. 2011

Appl Environ Microbiol

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We compare for the first time the electrokinetic and aggregation properties of MS2 phage (pH 2.5 to 7, 1 to 100 mM NaNO 3 electrolyte concentration) with those of the corresponding virus-like particles (VLPs), which lack entirely the inner viral RNA component. In line with our previous work (J. Langlet, F. Gaboriaud, C. Gantzer, and J. F. L. Duval, Biophys. J. 94:3293-3312, 2008), it is found that modifying the content of RNA within the virus leads to very distinct electrohydrodynamic and aggregation profiles for MS2 and MS2 VLPs. Under the given pH and concentration conditions, MS2 VLPs exhibit electrophoretic mobility larger in magnitude than that of MS2, and both have similar isoelectric point (IEP) values (ϳ4). The electrokinetic results reflect a greater permeability of MS2 VLPs to electroosmotic flow, developed within/around these soft particles during their migration under the action of the applied electrical field. Results also support the presence of some remaining negatively charged component within the VLPs. In addition, MS2 phage systematically forms aggregates at pH values below the IEP, regardless of the magnitude of the solution ionic strength, whereas MS2 VLPs aggregate under the strict condition where the pH is relatively equal to the IEP at sufficiently low salt concentrations (<10 mM). It is argued that the stability of VLPs against aggregation and the differences between electrokinetics of MS2 and corresponding VLPs conform to recently developed formalisms for the stability and electrohydrodynamics of soft multilayered particles. The differences between the surface properties of these two kinds of particles reported here suggest that VLPs may not be appropriate for predicting the behavior of pathogenic viruses in aqueous media.Understanding the behavior of virus particles of enteric pathogens (e.g., norovirus, hepatitis A virus) in terms of aggregation or adhesion is mandatory for addressing appropriately the processes involved in, e.g., viral dissemination or water treatment (9, 11). In that respect, much effort is now devoted to the analysis of the so-called surface properties of viruses, which are impacted by solution pH, ionic strength, or the presence of organic matter (20). The importance of viral surface properties within the framework of water treatment is well illustrated by the relationship between the efficiency of membrane filtration for removing viruses and the charge and degree of hydrophobicity of these biocolloids (11).According to standard formulations of Derjaguin-LandauVerwey-Overbeek (DLVO) representation and electrokinetic theories for hard (impermeable) particles, a parameter commonly used as an indicator for the sign and magnitude of charge carried by a virus in a solution of given pH is the difference between the pH and the so-called isoelectric point (IEP) of the virus, defined as the pH value at which virus electrophoretic mobility is zero (14). Within the regime of partial dissociation of charges located at the surface, the higher (or lower) the solution pH is than ...

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contrasting

confidence: 59%

Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles

Dika

Duval

Ly-Chatain

et al. 2011

Appl Environ Microbiol

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“…Additional factors affecting adsorption include the different intrinsic characteristics, such as the isoelectric point, of the virus. In this experiment, the viral surrogates studied (FCV, MS2, MNV, and PRD1) may have slightly different net surface charges, as demonstrated by the different isoelectric points (4.9, 3.9, unknown [but thought to be similar to that of NoV at 5.0], and 4.2, respectively) (38,52). Even with these differences, the elution buffer with a pH of 6.5 containing 0.3 M NaCl and 0.1% Tween 80 described here was formulated to inhibit electrostatic and hydrophobic interactions, thus disrupting adsorption to the surface.…”

Section: Discussionmentioning

confidence: 99%

Removal and Transfer of Viruses on Food Contact Surfaces by Cleaning Cloths

Gibson

Crandall

Ricke

2012

Appl Environ Microbiol

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ABSTRACTContamination of food contact surfaces with pathogens is considered an important vehicle for the indirect transmission of food-borne diseases. Five different cleaning cloths were assessed for the ability to remove viruses from food contact surfaces (stainless steel surface and nonporous solid surface) and to transfer viruses back to these surfaces. Cleaning cloths evaluated include two different cellulose/cotton cloths, one microfiber cloth, one nonwoven cloth, and one cotton terry bar towel. Four viral surrogates (murine norovirus [MNV], feline calicivirus [FCV], bacteriophages PRD1 and MS2) were included. Removal of FCV from stainless steel was significantly greater (P≤ 0.05) than that from nonporous solid surface, and overall removal of MNV from both surfaces was significantly less (P≤ 0.05) than that of FCV and PRD1. Additionally, the terry towel removed significantly fewer total viruses (P≤ 0.05) than the microfiber and one of the cotton/cellulose cloths. The cleaning cloth experiments were repeated with human norovirus. For transfer of viruses from cloth to surface, both cellulose/cotton cloths and microfiber transferred an average of 3.4 and 8.5 total PFU, respectively, to both surfaces, and the amounts transferred were significantly different (P≤ 0.05) from those for the nonwoven cloth and terry towel (309 and 331 total PFU, respectively). There was no statistically significant difference (P> 0.05) in the amount of virus transfer between surfaces. These data indicate that while the cleaning cloths assessed here can remove viruses from surfaces, some cloths may also transfer a significant amount of viruses back to food contact surfaces.

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“…Recent works used DLS for pI determination of adenovirus type 5 (Trilisky and Lenhoff, 2007) and retrovirus (Rodrigues et al, 2008); however, this is the first report where the pI of a VLP was determined using this technique. Also, Schaldach et al (2006) determined experimentally the pI of three viruses (Norwalk, MS2, and Qb) in order to study their interaction with charged surfaces. The great advantage of light scattering is the absence of virus staining, so that their surface charge remains unaffected.…”

mentioning

confidence: 99%

Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple‐layered rotavirus‐like particles

Mellado¹,

Mena²,

Lopes³

et al. 2009

Biotech & Bioengineering

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Virus-like particles constitute potentially relevant vaccine candidates. Nevertheless, their behavior in vitro and assembly process needs to be understood in order to improve their yield and quality. In this study we aimed at addressing these issues and for that purpose triple- and double-layered rotavirus-like particles (TLP 2/6/7 and DLP 2/6, respectively) size and zeta potential were measured using dynamic light scattering at different physicochemical conditions, namely pH, ionic strength, and temperature. Both TLP and DLP were stable within a pH range of 3-7 and at 5-25 degrees C. Aggregation occurred at 35-45 degrees C and their disassembly became evident at 65 degrees C. The isoelectric points of TLP and DLP were 3.0 and 3.8, respectively. In vitro kinetics of TLP disassembly was monitored. Ionic strength, temperature, and the chelating agent employed determined disassembly kinetics. Glycerol (10%) stabilized TLP by preventing its disassembly. Disassembled TLP was able to reassemble by dialysis at high calcium conditions. VP7 monomers were added to DLP in the presence of calcium to follow in vitro TLP assembly kinetics; its assembly rate being mostly affected by pH. Finally, DLP and TLP were found to coexist under certain conditions as determined from all reaction products analyzed by capillary electrophoresis. Overall, these results contribute to the design of new strategies for the improvement of TLP yield and quality by reducing the VP7 detachment from TLP.

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The influence of ionic strength on the interaction of viruses with charged surfaces under environmental conditions

Cited by 53 publications

References 29 publications

Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles

Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like Particles

Removal and Transfer of Viruses on Food Contact Surfaces by Cleaning Cloths

Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple‐layered rotavirus‐like particles

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