The influence of in ovo injection with the prebiotic DiNovo® on the development of histomorphological parameters of the duodenum, body mass and productivity in large-scale poultry production conditions

Abstract

BackgroundAmong various feed additives currently used in poultry nutrition, an important role is played by bioactive substances, including prebiotics. The beneficial effect of these bioactive substances on the gastrointestinal tract and immune system give rise to improvements in broiler health and performance nutrition, thus increasing the productivity of these birds. An innovative method for introducing bioactive substances into chickens is the in ovo injection into eggs intended for hatching. The aim of the study was to evaluate the development of histomorphological parameters of the duodenum and productivity in chickens injected in ovo with the prebiotic DiNovo® (extract of Laminaria species of seaweed, BioAtlantis Ltd., Ireland) on d 12 of incubation, under large - scale, high density poultry production conditions.ResultsThere was no significant impact of the injection of DiNovo® prebiotic on the production parameters of broiler chickens (body weight, FCR, EBI and mortality) obtained on d 42 of rearing. No significant impact of the DiNovo® injection on the duodenum weight and length was observed, as well as on the CSA, diameter and muscular layer thickness of the duodenum. The in ovo injection of DiNovo® significantly increased the width of the duodenal villi (P < 0.05) and crypt depth (P < 0.01) of chickens on d 21 of rearing. Other histomorphological parameters of duodenal villi at d 42 of chickens rearing such as: the height, width, and cross section area of villi were significantly greater in chickens from the control group compared to those from the DiNovo® group (P < 0.05 and P < 0.01).ConclusionsIn conclusion, this study demonstrates that injection of DiNovo® prebiotic into the air chamber of egg significantly influences the histomorphological parameters on d 21 of rearing without negatively affecting productivity in chickens at the end of rearing.

Microstructure of the small intestine in broiler chickens fed a diet with probiotic or synbiotic supplementation

“…Awad et al (), Awad et al () tested synbiotic and probiotic action in broiler chickens, obtaining significant enhancement of villus height and height/crypt ratio in the ileum and duodenum when compared to the control group, which is a significant achievement for broiler producers since short villi and deep crypts may lead to poor nutrient absorption, increased secretion and low performance. As expected, bioactive substances improved the structure of all three segments of the small intestine of broiler chickens, as in previous work performed by Sobolewska et al () and Bogucka et al ().…”

“…On to the control group, which is a significant achievement for broiler producers since short villi and deep crypts may lead to poor nutrient absorption, increased secretion and low performance. As expected, bioactive substances improved the structure of all three segments of the small intestine of broiler chickens, as in previous work performed by Sobolewska et al (2017) and Bogucka et al (2017).…”

In ovodelivery of various biological supplements, vaccines and drugs in poultry: current knowledge

“…Intra‐amniotic administration of synbiotics had a positive impact on feed conversion ratio at 22–42 days of age and 0–42 days, as well as on the proliferating cell nuclear antigen, villus height, and goblet and positive cell counts in broiler chickens . Prebiotics administered in ovo also resulted in a positive impact on the gut health of chicks and improving their overall development . Adult‐derived microbiota administered via the in ovo route has been shown to influence the development of microbiota in chickens along with the reduction in the prevalence of pathogenic bacteria .…”

“…113 Prebiotics administered in ovo also resulted in a positive impact on the gut health of chicks and improving their overall development. 114 Adult-derived microbiota administered via the in ovo route has been shown to influence the development of microbiota in chickens along with the reduction in the prevalence of pathogenic bacteria. 115 In ovo administration of Flora Max ® -B11 indicated a positive effect on hatchability of chickens, gain in BW, and fall in a count of Salmonella enteritidis.…”

Injection of Raffinose Family Oligosaccharides at 12 Days of Egg Incubation Modulates the Gut Development and Resistance to Opportunistic Pathogens in Broiler Chickens

“…To date, only few in ovo studies reported the application of prebiotics in ovo [6,7]. Regarding the performance objectives (body weight, feed intake and feed conversion ratio, FCR), the results for different prebiotics or synbiotics are found to be inconsistent, but consequently supporting the stimulating effect on gut development [8], immune gene expression [9], and improved resistance [10].…”