The influence of hydrostatic pressure on tissue engineered bone development

Neßler, K H L; Henstock, James R.; Haj, Alicia J. El; Waters, Sarah L.; Whiteley, Jonathan; Osborne, James M.

doi:10.1016/j.jtbi.2015.12.020

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…Nonlinear interactions could amplify these oscillations. For instance, some tissue scaffolds are cyclically loaded to facilitate cell growth via mechano-transduction (Angele et al, 2004;Nessler et al, 2016;. In certain parameter regimes, this oscillatory forcing could interact in non-trivial ways with these lattice induced oscillations, for instance inducing resonance in the cell density oscillations.…”

Section: Discussionmentioning

confidence: 99%

Lattice and continuum modelling of a bioactive porous tissue scaffold

Krause

Beliaev

Gorder

et al. 2018

Mathematical Medicine and Biology: A Journal of the IMA

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A contemporary procedure to grow artificial tissue is to seed cells onto a porous biomaterial scaffold and culture it within a perfusion bioreactor to facilitate the transport of nutrients to growing cells. Typical models of cell growth for tissue engineering applications make use of spatially homogeneous or spatially continuous equations to model cell growth, flow of culture medium, nutrient transport and their interactions. The network structure of the physical porous scaffold is often incorporated through parameters in these models, either phenomenologically or through techniques like mathematical homogenization. We derive a model on a square grid lattice to demonstrate the importance of explicitly modelling the network structure of the porous scaffold and compare results from this model with those from a modified continuum model from the literature. We capture two-way coupling between cell growth and fluid flow by allowing cells to block pores, and by allowing the shear stress of the fluid to affect cell growth and death. We explore a range of parameters for both models and demonstrate quantitative and qualitative differences between predictions from each of these approaches, including spatial pattern formation and local oscillations in cell density present only in the lattice model. These differences suggest that for some parameter regimes, corresponding to specific cell types and scaffold geometries, the lattice model gives qualitatively different model predictions than typical continuum models. Our results inform model selection for bioactive porous tissue scaffolds, aiding in the development of successful tissue engineering experiments and eventually clinically successful technologies.

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Section: Discussionmentioning

confidence: 99%

Lattice and continuum modelling of a bioactive porous tissue scaffold

Krause

Beliaev

Gorder

et al. 2018

Mathematical Medicine and Biology: A Journal of the IMA

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“…Studies have revealed that periodic HP promotes bone growth and organization in developmental models. 13 , 14 However, HP beyond the normal range, also being classified as pathological HP, can lead to decompensated lesions in tissues and organs, such as bladder fibrosis and decreased sperm quality. 15 Osteogenesis and bone density can also be enhanced by HP.…”

Section: Characteristics and Mechanisms Of Cellular Mechanotransductionmentioning

confidence: 99%

Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets

Gao

et al. 2023

Sig Transduct Target Ther

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Cellular mechanotransduction, a critical regulator of numerous biological processes, is the conversion from mechanical signals to biochemical signals regarding cell activities and metabolism. Typical mechanical cues in organisms include hydrostatic pressure, fluid shear stress, tensile force, extracellular matrix stiffness or tissue elasticity, and extracellular fluid viscosity. Mechanotransduction has been expected to trigger multiple biological processes, such as embryonic development, tissue repair and regeneration. However, prolonged excessive mechanical stimulation can result in pathological processes, such as multi-organ fibrosis, tumorigenesis, and cancer immunotherapy resistance. Although the associations between mechanical cues and normal tissue homeostasis or diseases have been identified, the regulatory mechanisms among different mechanical cues are not yet comprehensively illustrated, and no effective therapies are currently available targeting mechanical cue-related signaling. This review systematically summarizes the characteristics and regulatory mechanisms of typical mechanical cues in normal conditions and diseases with the updated evidence. The key effectors responding to mechanical stimulations are listed, such as Piezo channels, integrins, Yes-associated protein (YAP) /transcriptional coactivator with PDZ-binding motif (TAZ), and transient receptor potential vanilloid 4 (TRPV4). We also reviewed the key signaling pathways, therapeutic targets and cutting-edge clinical applications of diseases related to mechanical cues.

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“…Our objective in this investigation was to develop a 4D approach in which the individual 3D printed microscaffold components self-assemble after arthroscopic injection into a final structure which is stabilised by both the designed microscaffold geometry and by the action of embedded cells in response to the environmental conditions. Our hypothesis was that the addition of live, reactive cells into micropores within the material would enable the 3D printed microscaffolds to facilitate improved healing over time in response to the wound microenvironment, including the biochemical signals from surrounding tissue, and in response to the mechanical requirements as the joint returns to function through extracellular matrix organisation [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

A 4D printed self-assembling PEGDA microscaffold fabricated by digital light processing for arthroscopic articular cartilage tissue engineering

Hao

et al. 2022

Prog Addit Manuf

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Articular cartilage in synovial joints such as the knee has limited capability to regenerate independently, and most clinical options for focal cartilage repair merely delay total joint replacement. Tissue engineering presents a repair strategy in which an injectable cell-laden scaffold material is used to reconstruct the joint in situ through mechanical stabilisation and cell-mediated regeneration. In this study, we designed and 3D-printed millimetre-scale micro-patterned PEGDA biomaterial microscaffolds which self-assemble through tessellation at a scale relevant for applications in osteochondral cartilage reconstruction. Using simulated chondral lesions in an in vitro model, a series of scaffold designs and viscous delivery solutions were assessed. Hexagonal microscaffolds (750 μm x 300 μm) demonstrated the best coverage of a model cartilage lesion (at 73.3%) when injected with a 1% methyl cellulose solution. When chondrocytes were introduced to the biomaterial via a collagen hydrogel, they successfully engrafted with the printed microscaffolds and survived for at least 14 days in vitro, showing the feasibility of reconstructing stratified cartilaginous tissue using this strategy. Our study demonstrates a promising application of this 4D-printed injectable technique for future clinical applications in osteochondral tissue engineering.

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The influence of hydrostatic pressure on tissue engineered bone development

Cited by 11 publications

References 44 publications

Lattice and continuum modelling of a bioactive porous tissue scaffold

Lattice and continuum modelling of a bioactive porous tissue scaffold

Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets

A 4D printed self-assembling PEGDA microscaffold fabricated by digital light processing for arthroscopic articular cartilage tissue engineering

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