The influence of housing condition on autotomy following dorsal rhizotomy in rats

Berman, Doreen; Rodin, Barbara E.

doi:10.1016/0304-3959(82)90020-3

Cited by 45 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the surgery, individual rats were housed in the presence of a female rat because this practice has been shown to prevent autotomy (Berman and Rodin, 1982).…”

Section: Light Microscopic Immunolabeling Of Rat Brain Sectionsmentioning

confidence: 99%

Morphine-Induced Changes in δ Opioid Receptor Trafficking Are Linked to Somatosensory Processing in the Rat Spinal Cord

et al. 2004

View full text Add to dashboard Cite

An in vivo fluorescent deltorphin (Fluo-DLT) internalization assay was used to assess the distribution and regulation of pharmacologically available ␦ opioid receptors (␦ORs) in the rat lumbar (L4 -5) spinal cord. Under basal conditions, intrathecal injection of Fluo-DLT resulted in the labeling of numerous ␦OR-internalizing neurons throughout dorsal and ventral horns. The distribution and number of Fluo-DLT-labeled perikaryal profiles were consistent with that of ␦OR-expressing neurons, as revealed by in situ hybridization and immunohistochemistry, suggesting that a large proportion of these cells was responsive to intrathecally administered ␦OR agonists. Pretreatment of rats with morphine for 48 hr resulted in a selective increase in Fluo-DLT-labeled perikaryal profiles within the dorsal horn. These changes were not accompanied by corresponding augmentations in either ␦OR mRNA or 125 I-deltorphin-II binding levels, suggesting that they were attributable to higher densities of cell surface ␦OR available for internalization rather than to enhanced production of the receptor. Unilateral dorsal rhizotomy also resulted in increased Fluo-DLT internalization in the ipsilateral dorsal horn when compared with the side contralateral to the deafferentation or to non-deafferented controls, suggesting that ␦OR trafficking in dorsal horn neurons may be regulated by afferent inputs. Furthermore, morphine treatment no longer increased Fluo-DLT internalization on either side of the spinal cord after unilateral dorsal rhizotomy, indicating that OR-induced changes in the cell surface availability of ␦OR depend on the integrity of primary afferent inputs. Together, these results suggest that regulation of ␦OR responsiveness through OR activation in this region is linked to somatosensory information processing.

show abstract

“…After the surgery, individual rats were housed in the presence of a female rat because this practice has been shown to prevent autotomy (Berman and Rodin, 1982).…”

Section: Light Microscopic Immunolabeling Of Rat Brain Sectionsmentioning

confidence: 99%

Morphine-Induced Changes in δ Opioid Receptor Trafficking Are Linked to Somatosensory Processing in the Rat Spinal Cord

et al. 2004

View full text Add to dashboard Cite

show abstract

“…All operations were carried out under sterile conditions. To prevent the autotomy behavior that often occurs in rhizotomized rats, each animal was housed with an unoperated female rat (Berman and Rodin, 1982). Under these conditions, postoperative recovery was rapid and autotomy was avoided.…”

Section: Animals and Surgerymentioning

confidence: 99%

Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Taquet

Plachot

Pohl

et al. 1992

J. Neural Transmission

View full text Add to dashboard Cite

Possible changes in neuropeptides within the ventral horn of the spinal cord were investigated after unilateral dorsal rhizotomy at the lumbar level (L1-L6) in adult rats. Ten days after the surgery, immunohistochemical observations and radioimmunological determinations confirmed a marked loss of calcitonin gene-related peptide (CGRP)- and substance P (SP)-like immunoreactivities within the superficial layers of the deafferented dorsal horn, as expected from the degeneration of primary afferent fibres containing these peptides. A concomitant increase in immunohistochemical staining and levels of CGRP (+296%) and CCK (+71%)-like immunoreactivities was observed in the ipsilateral ventral horn where both peptides are located in motoneurones. In contrast, substance P-like immunoreactivity that is confined to fibres and terminals within the ventral horn, was not altered by dorsal rhizotomy. These data indicate that the expression of neuropeptides in spinal motoneurones can be influenced by primary afferent inputs.

show abstract

“…Twenty-six female albino rats (Harlan Sprague-Dawley Inc., Indianapolis, IN; 3-6 months of age) were housed two per cage to reduce the incidence of autotomy (Berman and Rodin, 1982). Animals were maintained in a 12 h light/dark cycle, with water and food freely available.…”

Section: Subjectsmentioning

confidence: 99%

Spinal Cord Injury Produced by Consistent Mechanical Displacement of the Cord in Rats: Behavioral and Histologic Analysis

Behrmann

Bresnahan²,

Beattie³

et al. 1992

Journal of Neurotrauma

226

113

View full text Add to dashboard Cite

We examined the ability of an electromechanical device to produce consistent and incomplete thoracic (T9) spinal cord injuries in rats by brief displacement (Dspl) of the exposed dural surface. Open field walking, inclined plane, grid walking, and footprint analysis, and a determination of the percentage of tissue spared at the lesion center were used to assess chronic outcome (6 weeks postinjury). Laminectomy control animals showed no evidence of a functional deficit or histologic lesion. Complete spinal cord transections in normal rats and in a group of animals previously injured (1.1 mm Dspl) and allowed to recover resulted in complete loss of hindlimb function, demonstrating an important functional role for the remaining spared fibers at the lesion site. Consistent spinal cord displacements (0.80 mm, 0.95 mm, and 1.10 mm) resulted in behavioral groups with low outcome variability over a narrow range of incomplete recovery of neurologic function. Significant behavioral (open field walking, inclined plane, and grid walking) and histologic differences were found between the control and Dspl groups and between the 0.80 mm and 1.10 mm Dspl groups. Significant correlations were observed among the injury parameters, behavioral, and histologic scores. Open field walking and inclined plane performance were sensitive indicators of both the early and late phases of neurologic recovery. Grid walking was most useful in animals with small chronic residual deficits. The footprint analysis resulted in less significant correlations and differences between the behavioral groups than the other outcome measures. This may result from a relatively narrow range of sensitivity (open field walking scores between 3.3 and 4.0) and increased variability within the groups.

show abstract

The influence of housing condition on autotomy following dorsal rhizotomy in rats

Cited by 45 publications

References 15 publications

Morphine-Induced Changes in δ Opioid Receptor Trafficking Are Linked to Somatosensory Processing in the Rat Spinal Cord

Morphine-Induced Changes in δ Opioid Receptor Trafficking Are Linked to Somatosensory Processing in the Rat Spinal Cord

Increased calcitonin gene-related peptide- and cholecystokinin-like immunoreactivities in spinal motoneurones after dorsal rhizotomy

Spinal Cord Injury Produced by Consistent Mechanical Displacement of the Cord in Rats: Behavioral and Histologic Analysis

Contact Info

Product

Resources

About