The influence of homogeneous cold agglutinins on polymorphonuclear and mononuclear phagocytes

Pruzanski, W.; Farid, N.R.; Keystone, Edward; Armstrong, M.

doi:10.1016/0090-1229(75)90063-x

Cited by 47 publications

(16 citation statements)

References 16 publications

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“…The second group, composed of three sera (Nos. [4][5][6], reacted equally with the papain-treated adult and cord RBC. These sera also reacted with neuraminidasetreated RBC.…”

Section: Resultsmentioning

confidence: 99%

Cryptic cold agglutinin activity of monoclonal macroglobulins

et al. 1987

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One hundred and twenty sera with monoclonal immunoglobulin M's (IgM's) were tested for cryptic cold agglutinin activity against papain- and neuraminidase-treated adult and cord red blood cells (RBC). Fifteen sera (12.5%) agglutinated papain-treated RBC. Fourteen of these 15 sera also agglutinated neuraminidase-treated RBC. Ten additional sera agglutinated neuraminidase-treated but not papain-treated cells. Three patterns were observed among agglutinators of papain-treated RBC: either predominant agglutination of adult RBC, or of cord RBC or equal reaction, thus resembling I, i and non-Ii patterns of conventional cold agglutinins. We propose to call these patterns, tentatively, Icr (cr for cryptic), icr, and non-Iicr, respectively. Purified monoclonal IgM's retained the above patterns. Eluates from enzyme-treated cells contained monoclonal IgM's. Investigation of neuraminidase-treated RBC has shown that in distinction to T-anti-T system (Thomsen-Friedenreich receptor system), the majority of sera with monoclonal IgM's agglutinated more strongly adult RBC, and some agglutinated more strongly cord RBC. There was no loss of agglutinating activity after repeat freezing and thawing of the sera. Thus, it seems that monoclonal IgM's which react with neuraminidase-treated RBC detect antigenic determinants different from the T antigen. Cryptic CA activity of IgM's should be added to the list of antibody activities of these monoclonal immunoglobulins.

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“…The second group, composed of three sera (Nos. [4][5][6], reacted equally with the papain-treated adult and cord RBC. These sera also reacted with neuraminidasetreated RBC.…”

Section: Resultsmentioning

confidence: 99%

Cryptic cold agglutinin activity of monoclonal macroglobulins

et al. 1987

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“…Neutrophils were isolated from the peripheral blood of the patient before chemotherapy was started and purified, as described [4]. Their purity was tested by morphology and myeloperoxidase (51, nonspecific esterase and chloroacetate esterase stains [6], and viability was tested by the trypan-blue exclusion method.…”

Section: Methodsmentioning

confidence: 99%

“…Their purity was tested by morphology and myeloperoxidase (51, nonspecific esterase and chloroacetate esterase stains [6], and viability was tested by the trypan-blue exclusion method. Phagocytic assays were performed by previously published methods [4]. Briefly, the neutrophils were washed twice at room temperature in Hank's balanced salt solution (HBSS) and mixed with a suspension of viable Staphylococcus aureus or Streptococcus fecalis in the ratio of 20 bacteria to 1 cell, with or without fresh human serum.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were removed by centrifugation and the supernatants were assayed for enzyme activity [S]. Susceptibility of neutrophils to cytotoxic activity of cold agglutinins, of SLE sera, and of cerebrospinal fluids was tested by the methods developed in our laboratory [4,9,10] using bithermic assays (4°C for 60 min and 24°C for 60 min) and a nontoxic rabbit serum as a source of complement. Colony-forming capacity of the bone marrow and the peripheral blood granulocytes was tested by the methods described by Fauser and Messner [I 11.…”

Section: Methodsmentioning

confidence: 99%

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Functional characterization of the cells in chronic neutrophilic leukemia

1982

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Light and electron microscopy of neutrophils from chronic neutrophilic leukemia (CNL) did not reveal differences from normal mature neutrophils. However, functional characterization of CNL cells showed marked differences when compared to normal cells. CNL neutrophils were much less viable in suboptimal conditions. Their survival was further reduced by autologous serum and was corrected by normal human serm. CNL cells showed very active phagocytosis, but their bactericidal activity was reduced in suboptimal conditions. The total content of lysozyme and beta-glucuronidase was lower in CNL cells compared to normal neutrophils, but the release of these enzymes from stimulated cells was much higher than normal. This observation is compatible with a marked lysosomal lability. Cells from the patients' peripheral blood and bone marrow showed excessive growth in CFU-C assays. Marked susceptibility of CNL cells to cytotoxic activity of cold agglutinins, SLE sera, and CSFs was observed and may signify qualitative and/or quantitative differences in the membrane structure of CNL neutrophils, as compared to normal cells.

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“…Nach unseren Literaturkenntnissen ist aber ein der Pseudothrombopenie ähnlicher Befund für Leukocyten bisher nicht beschrieben. Allerdings wird über Antikörper der Spezifität I und i berichtet (Kälteagglutinine), die eine cytotoxische Wirkung haben und zwischen 7 und 76% der polymorphkernigen Leukocyten in vitro abtöten (9).…”

Section: Histogrammeunclassified

Analytische und technische Zuverlässigkeit des ELT-8 Laser Hämatologie Zellzählers

Bauersfeld¹,

Knoll²,

Wisser³

1985

Clinical Chemistry and Laboratory Medicine

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Analyticäl and technical reliäbility of the ELT-8 laser haematological counterSünimary: The ELT-8 laser haematological cell counter is a fully mechanized Instrument, which measures the forward scatter of light from a helium-neon laser caused by suspended particles.The apparatus has high practicability, and uses a very small sample volume of 100 . The operational proeedure is simple and quickly learned. In the second year of Operation, down time for the Instrument was, on average, 8 hours/month.Depending pn the parameter, the precision of the measurements are between 0.5 and 4% in series, and l -9% day-to-day. The highest scatter of results was shown for thrombocyte counting, but this may be due to instability of the control samples. There was no carry over between samples containing different concentrations of any of «the measured parameters. Lipaemia and haemolysis of samples can lead to false results

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The influence of homogeneous cold agglutinins on polymorphonuclear and mononuclear phagocytes

Cited by 47 publications

References 16 publications

Cryptic cold agglutinin activity of monoclonal macroglobulins

Cryptic cold agglutinin activity of monoclonal macroglobulins

Functional characterization of the cells in chronic neutrophilic leukemia

Analytische und technische Zuverlässigkeit des ELT-8 Laser Hämatologie Zellzählers

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