The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing ileal transposition

Sawczyn, Tomasz; Stygar, Dominika; Nabrdalik, Katarzyna; Kukla, Michał; Skrzep-Poloczek, Bronisława; Wesołowski, Bartosz; Olszańska, Ewa; Dulska, Agnieszka; Gumprecht, Janusz; Karcz, Wojciech Konrad; Jochem, Jerzy

doi:10.1016/j.peptides.2019.04.001

Cited by 2 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high ANGPTL8 plasma level seems to be considered an advantage of the IT surgery, as observed in groups preoperatively fed HFD. However, our recent study showed no significant differences in HOMA-IR and insulin plasma levels between the IT and SHAM operated animals and the different diets [ 37 ]. ANGPTL8 is primarily expressed in the liver and adipose tissue, from where it is secreted into the bloodstream.…”

Section: Discussionmentioning

confidence: 99%

“…It is worth mentioning that IT operated rats fed HFD postoperatively had higher HOMA-IR levels than rats fed CD. In addition, in originally normoglycemic rats that underwent IT surgery and changed to HFD, the incretin effect was significantly impaired or did not occur at all [ 37 ]. Bonacina et al [ 28 ] received increased PTX3 levels and impaired glucose tolerance and insulin sensitivity after feeding mice with HFD for 10 and later 20 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…All diets were purchased from Ssniff Spezialdiäten GmbH (Soest, Germany). Diet composition, expressed as a percentage of metabolisable energy (ME), was the same as in our previous study [ 37 ]: control diet (referred to as CD in the following) (D12450 B I mod. ; Ssniff): 9% from fat, 26% from protein, and 65% from carbohydrates (ME = 3,77 kcal/g); HFD (D12492 II mod.…”

Section: Methodsmentioning

confidence: 99%

“…After the operation, animals were maintained on a liquid diet (Nutrison, Nutricia, Poland) for 24 hours. Following this period, when the rats did not present any symptoms of postoperative complications, they were randomly assigned to groups maintained on varied postoperative diets [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…All statistical procedures were performed as in our earlier study [ 37 ]. Briefly, the concentration of plasma hepatokines and adipokines did not fulfil the criteria of normal distribution (Shapiro–Wilk test).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)

Sawczyn

Stygar

Nabrdalik

et al. 2021

International Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Background. Metabolic surgery procedures are designed not only for sustained weight loss but also for achieving positive metabolic changes, including improved glucose tolerance and insulin sensitivity, along with an increase in energy expenditure. Based on recent findings, the present study focuses on the relationship between the effects of ileal transposition (IT), high-fat diet (HFD), and selected markers of lipid metabolism and inflammation. Methods. Forty-eight male rats were divided into two groups: HFD and control diet (CD) fed rats. After eight weeks, animals in each group were randomly assigned to two types of surgery: IT and SHAM. Thereafter, fifty percent of the animals in the HFD and CD groups had their diets changed, while the remaining half maintained their presurgery diets. Eight weeks after surgery, plasma levels of ANGPTL8, PTX3, leptin, and adiponectin were assessed. Results. The IT group pre- and postoperatively maintained on the HFD showed higher ANGPTL8 level compared to SHAM operated animals ( p = 0.0041 ). The effect of IT on PTX3 level in the group pre- and postoperatively maintained on a CD was not significant, and there were no differences compared to SHAM. Only the postoperative diet change to HFD increased PTX3 level in the IT operated animals ( p = 0.0002 ). The IT group had increased plasma adiponectin ( p = 0.026 ) and leptin ( p = 0.0027 ) levels after dietary change to HFD compared to IT rats fed CD. Conclusions. This study indicates that the outcomes of metabolic surgery can be greatly modified by HFD. The effects of the IT procedure in this experiment are ambiguous and do not provide a clear answer as to whether or not they are beneficial.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)

Sawczyn

Stygar

Nabrdalik

et al. 2021

International Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl:ZUC(ORL)-Leprfa) rats

Stygar

Sawczyn

Dulska

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

We studied the long-term effect of ileal transposition (IT) metabolic surgery on the hepatokines: retinol-binding protein-4 (RBP4), α-2-HS-glycoprotein (aHSG/fetuin-A), and fibroblast growth factor 21 (FGF21), C-reactive protein (CRP) plasma levels, glucose metabolism, body weight, liver histology, as well as total lipids concentration in muscle, liver, and fat tissue of obese Zucker (Crl:ZUC(ORL)-Leprfa) rats. 14 adult males were randomly submitted either to IT or SHAM (control) surgery. Pre-operative hepatokines plasma levels were not significantly different in rats submitted to IT or SHAM protocol. Three months after the procedures the plasma levels of RBP4, aHSG, FGF21, and CRP were significantly lower in IT-operated animals when compared to SHAM-operated group. Three and 12 weeks after the IT and SHAM surgery, the AUCOGTT were significantly lower than AUCOGTT before the surgery. HOMA-IR was lower in rats after IT surgery in comparison to the SHAM-operated rats. Muscle and liver total lipids concentration was reduced after the IT procedure when compared to pre-IT conditions. IT had a significant reductive impact on the body weight in comparison to SHAM surgery in the 4th, 6th, 8th, and 10th week after the surgery. We conclude that IT reduces hepatokines’ plasma concentrations, muscle and liver total lipids concentration but not the inflammatory processes in the liver of Zucker (Crl:ZUC(ORL)-Leprfa) rats.

show abstract

The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing ileal transposition

Cited by 2 publications

References 44 publications

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)

Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl:ZUC(ORL)-Leprfa) rats

Contact Info

Product

Resources

About