The influence of gold surface texture on microglia morphology and activation

Tan, Yih Horng; Terrill, Shana E.; Paranjape, Geeta; Stine, Keith J.; Nichols, Michael R.

doi:10.1039/c3bm60096c

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…This method has been used to create np-Au material libraries consisting of several different morphologies on a single chip. These libraries have the potential to drastically increase the throughput of morphology interaction studies for np-Au specifically in applications such as surface enhanced Raman spectroscopy (SERS), 34 high capacity lithium ion batteries, 35 cell-material interaction studies for neural interfaces, 12, 36 analytical biosensors, 37 as well as nanoscale material science studies. 38 Further optimization of both the material selection (i.e.…”

Section: Resultsmentioning

confidence: 99%

Engineering on-chip nanoporous gold material libraries via precision photothermal treatment

et al. 2016

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Libraries of nanostructured materials on a single chip are a promising platform for high throughput and combinatorial studies of structure-property relationships in the fields of physics and biology. Nanoporous gold (np-Au), produced by an alloy corrosion process, is a nanostructured material specifically suited for such studies because of its self-similar thermally induced coarsening behavior. However, traditional heat application techniques for the modification of np-Au are bulk processes cannot be used to generate a library of different pore sizes on a single chip. Here, laser micro-processing offers an attractive solution to this problem by providing a means to apply energy with high spatial and temporal resolution. In the present study we use finite element multiphysics simulations to predict the effects of laser mode (continuous-wave vs. pulsed) and thermal conductivity of the supporting substrate on the local np-Au film temperatures during photothermal annealing. Based on these results we discuss the mechanisms by which the np-Au network is coarsened. Thermal transport simulations predict that continuous-wave mode laser irradiation of np-Au thin films on a silicon substrate supports the widest range of morphologies that can be created through the photothermal annealing of np-Au. Using the guidance provided by simulations, we successfully fabricate an on-chip material library consisting of 81 np-Au samples of 9 different morphologies for use in the parallel study of structure-property relationships.

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Section: Resultsmentioning

confidence: 99%

Engineering on-chip nanoporous gold material libraries via precision photothermal treatment

et al. 2016

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“…Whether it also has an influence on the cellular adhesion to the surface remains to be investigated since there is no conclusive result in the literature stating what the optimal topography would be from this perspective. In a couple of recent studies, gold nanoparticle coated surfaces were studied with regard to their ability to preferentially anchor neurons instead of astrocytes and to mitigate the proliferation of microglial cells [32,33]. The conclusion was that nanostructures do indeed influence which cells that adhere and that the proportion of neurons in comparison to astrocytes were significantly higher on the nanostructured surfaces in comparison to the flat ones.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 97%

Nanostructured platinum grass enables superior impedance reduction for neural microelectrodes

2015

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Micro-sized electrodes are essential for highly sensitive communication at the neural interface with superior spatial resolution. However, such small electrodes inevitably suffer from high electrical impedance and thus high levels of thermal noise deteriorating the signal to noise ratio. In order to overcome this problem, a nanostructured Pt-coating was introduced as add-on functionalization for impedance reduction of small electrodes. In comparison to platinum black deposition, all used chemicals in the deposition process are free from cytotoxic components. The grass-like nanostructure was found to reduce the impedance by almost two orders of magnitude compared to untreated samples which was lower than what could be achieved with conventional electrode coatings like IrOx or PEDOT. The realization of the Pt-grass coating is performed via a simple electrochemical process which can be applied to virtually any possible electrode type and accordingly shows potential as a universal impedance reduction strategy. Elution tests revealed non-toxicity of the Pt-grass and the coating was found to exhibit strong adhesion to the metallized substrate.

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“…Therefore, a significant research effort has been devoted to controlling cell fate (e.g., phenotypic changes, adhesion, migration) by manipulating purely mechanical cues from substrate surfaces . Although much of the research in this field has been directed toward controlling stem cell fate and differentiation by varying substrate stiffness and material nanostructure, using mechanical cues to drive neural cell coupling presents an attractive means to improve the signal fidelity of neural interfaces . Moving toward the development of neural recording interfaces with high fidelity and long‐term stability requires an electrode interface that maintains close physical coupling between neurons and the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance

et al. 2016

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Nanostructured neural interface coatings have significantly enhanced recording fidelity in both implantable and in vitro devices. As such, nano-porous gold (np-Au) has shown promise as a multifunctional neural interface coating due, in part, to its ability to promote nanostructure-mediated reduction in astrocytic surface coverage while not affecting neuronal coverage. The goal of this study is to provide insight into the mechanisms by which the np-Au nanostructure drives the differential response of neurons versus astrocytes in an in vitro model. Utilizing microfabricated libraries that display varying feature sizes of np-Au, it is demonstrated that np-Au influ-ences neural cell coverage through modulating focal adhesion formation in a feature size-dependent manner. The results here show that surfaces with small (≈30 nm) features control astrocyte spreading through inhibition of focal adhesion formation, while surfaces with large (≈170 nm and greater) features control astrocyte spreading through other mechanotransduction mechanisms. This cellular response combined with lower electrical impedance of np-Au electrodes significantly enhances the fidelity and stability of electrophysiological recordings from cortical neuronglia co-cultures relative to smooth gold electrodes. Finally, by leveraging the effect of nanostructure on neuronal versus glial cell attachment, the use of laser-based nanostructure modulation is demonstrated for selectively patterning neurons with micrometer spatial resolution.

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The influence of gold surface texture on microglia morphology and activation

Cited by 27 publications

References 36 publications

Engineering on-chip nanoporous gold material libraries via precision photothermal treatment

Engineering on-chip nanoporous gold material libraries via precision photothermal treatment

Nanostructured platinum grass enables superior impedance reduction for neural microelectrodes

Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance

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