The influence of glycerol upon L. reuteri activity against
            enteropathogens

Etchebehere, Marina Camargo; Piveta, Cristiane S C; Levy, Carlos Emílio

doi:10.5935/medicalexpress.2017.06.06

Cited by 3 publications

(3 citation statements)

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“…Research interest in this probiotic is due not only to its well-known immunotropic, anti-inflammatory and antimicrobial activity. Recent attention of researchers has been focused on the L. reuteri as a producer of reuterin, which is a precursor-induced antimicrobial agent [18,19].…”

Section: ключові словаmentioning

confidence: 99%

“…However, for complete growth inhibition, for example, enterobacteria L. reuteri requires at least 0.2 % of glycerol. These circumstances should encourage researchers to reconsider existing methods of using probiotic supplements for more effective clinical use of their antimicrobial potential [18]. Precursor-directed antimicrobial biosynthesis is considered as a promising strategy for next-generation probiotics development [19].…”

Section: Original Researchmentioning

confidence: 99%

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Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Knysh

Martynov

2020

ZMJ

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The aim of the research was to evaluate the antimicrobial potential of cell-free extracts obtained in various ways from the probiotic strain Lactobacillus reuteri DSM 17938 with respect to their ability to influence the proliferation of antibiotic-resistant bacteria.Materials and methods. Cell-free extracts were obtained: 1) from L. reuteri cell suspension, subjected to disintegration by repeated freezing-thawing, L; 2) from L. reuteri culture, cultivated in its own disintegrate (ML); 3) from L. reuteri culture, cultivated in its own disintegrate supplemented with glycerol (73.7 mg/ml) and glucose (72.1 mg/ml) (MLG); 4) from L. reuteri culture, cultivated in its own disintegrate supplemented with ascorbic acid (20 mg/ml) (MLA). Multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical isolates: Escherichia coli, Klebsiella pneumoniae, Lelliottia amnigena and Corynebacterium xerosis were used as a test cultures. The investigation of the inhibitory activity of cell-free extracts was carried out by spectrophotometric method using a microplate analyzer "Lisa Scan EM" ("Erba Lachema s.r.o.", Czech Republic).Results. Cell-free extract L exerted predominantly stimulatory effect on the proliferation of all studied test cultures. Cell-free extract ML had significant inhibitory effect on the proliferation of E. coli and C. xerosis (growth inhibition indices were 24.8 % and 96.1 %, respectively) and did not have significant effect on the proliferation of K. pneumoniae and L. amnigena. Cell-free extracts MLG and MLA caused pronounced inhibition of the proliferative activity of all tested microorganisms. Growth inhibition indices were: 75.0 % and 90.7 % (E. coli), 77.9 % and 88.9 % (K. pneumoniae), 40.9 % and 77.9 % (L. amnigena), 99 % and 100 % (C. xerosis), respectively.Conclusions. The cell-free extracts obtained by cultivation of L. reuteri DSM 17938 in its own disintegrate supplemented with glycerol and glucose or ascorbic acid have shown a pronounced antimicrobial activity against antibiotic-resistant bacteria in vitro. After confirming safety and efficacy in vivo, they can be used to increase the efficiency of the therapy of diseases caused by antibiotic-resistant microorganisms. The results of the study indicate the prospects of obtaining probiotic derivatives with high antimicrobial activity by applying a combinatorial (precursor directed) biosynthesis strategy.

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Section: ключові словаmentioning

confidence: 99%

Section: Original Researchmentioning

confidence: 99%

Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Knysh

Martynov

2020

ZMJ

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“…A atividade antimicrobiana dos probióticos contra KPC e outras enterobactérias patogênicas foi realizado pelo método de co-cultura, sendo que para o L. reuteri foi adicionado 1% de glicerol (74).…”

Section: Atividade Antimicrobiana Dos Probióticos Contra Klebsiella P...unclassified

Atividade antimicrobiana in vitro de probióticos em Klebsiella pneumoniae produtora de carbapenemase, Escherichia coli, Salmonella enteritidis e Shigella flexneri

Freire Gasparetto

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Agradeço imensamente ao meu orientador Professor Dr. Carlos Emilio Levy pela oportunidade de aprender mais a fundo sobre a área da microbiologia, pela confiança em mim depositada e por acreditar no meu potencial. Obrigada por toda dedicação e paciência, e obrigada por todos os ensinamentos passados ao longo desse processo.Aos funcionários do Laboratório de Microbiologia da divisão de Patologia Clínica do HC-Unicamp, em especial à Cristiane Piveta, por dividirem seu espaço de trabalho, por transmitirem seus conhecimentos e pela ajuda sempre que solicitado.

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Potentiation of the antimicrobial effect of Lactobacillus reuteri DSM 17938 cell-free extracts by ascorbic acid.

Knysh¹,

Martynov²

2020

Medicni Perspektivi (Medical Perspectives)

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Potentiation of the antimicrobial effect of Lactobacillus reuteri DSM 17938 cell-free extracts by ascorbic acid. Knysh O.V., Martynov A.V. The purpose of this study was to evaluate the effect of supplementing the culture medium with ascorbic acid on the antimicrobial properties of Lactobacillus reuteri cell-free extracts (CFEs). CFEs were prepared using commercial strain L. reuteri DSM 17938 by culturing lactobacilli in its own disintegrated cell suspension (DCS) supplemented with ascorbic acid in sub-inhibitory (5 mg/ml, CFE 5 ) or minimal inhibitory concentration (20 mg/ml, CFE 20 ) and without supplementation (CFE 0 ). Staphylococcus aureus AТСС 25923, Escherichia coli AТСС 25922 reference strains and Pseudomonas aeruginosa extensively drug resistant (XDR) clinical isolate were used as indicator cultures. Screening of the inhibitory properties of the studied CFEs and elucidation of the nature of inhibitory products were done using modified Micro scale Optical Density Assay (MODA). The inhibition indices (InhI) were calculated for the studied CFEs and ascorbic acid of appropriate concentrations. CFEs were subjected to HPLC-analysis. CFE 5 and CFE 20 showed significantly higher antimicrobial activity toward to indicator cultures than CFE 0 . InhI calculated for extracts CFE 5 and CFE 20 mainly exceeded the sum of the corresponding indicators calculated for CFE 0 and ascorbic acid (АА) of appropriate concentrations: InhI CFE 5 ≥ InhI CFE 0 + InhI AA 5 ; InhI CFE 20 > InhI CFE 0 + InhI AA 20. Acidic metabolic products have made the greatest contribution to the antimicrobial effect of the studied CFEs. HPLC-nalysis showed that the modified ascorbic acid was the substance found in CFE 20 in the greatest quantity. The revealed effect of potentiation of antimicrobial activity of CFEs by ascorbic acid should be taken into account when developing new biotechnological products based on derivatives of L. reuteri DSM 17938.

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The influence of glycerol upon L. reuteri activity against enteropathogens

Cited by 3 publications

References 5 publications

Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Atividade antimicrobiana in vitro de probióticos em Klebsiella pneumoniae produtora de carbapenemase, Escherichia coli, Salmonella enteritidis e Shigella flexneri

Potentiation of the antimicrobial effect of Lactobacillus reuteri DSM 17938 cell-free extracts by ascorbic acid.

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