The influence of genomes on autonomous growth of pith cultures of Nicotiana glauca-Langsdorffii hybrids

Cheng, Tsai-Ying; Smith, Harold H.

doi:10.1007/bf00385186

Cited by 12 publications

(2 citation statements)

References 4 publications

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“…It is clear that even in species considered to be relatively easy to culture in tissue culture, the genotype of the plant may exert a significant influence on culture results. For example in "model" species such as Nicotiana, Cheng and Smith (1973) found noticeable differences among genomes for autonomous growth of pith cultures. Similarly dramatic differences in shoot numbers were produce<I from leaf segment cultures of a range of petunia cultivars (Read, et al, 1979).…”

Section: Explant Selectionmentioning

confidence: 98%

Environmental and Hormonal Effects in Micropropagation

Read

1992

Transplant Production Systems

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ABSTRACT. Micropropagation has become important in the latter quarter of the Twentieth Century, both in a commercial sense and as an exceedingly valuable research tool for the study of plant physiology, growth and development. A myriad of papers have been published on a wide variety of subjects related to micropropagation including the critically important areas of environment and hormones. In this paper, special attention is given to the influence of environment and hormones, and their interactions as they affect the stock plant and the subsequent performance of the explant when cultured in vitro. Explant selection (genotype, organ chosen, age and physiological stage, health), stock plant management (nutrition; light intensity, quality and photoperiod; temperature; chemical and physical manipulations), gaseous atmosphere (RH, CO2, O2, ethylene) and both conventioml and novel plant growth regulating chemicals are topics presented. These topics are discussed in relation to future challenges and opportunities, such as automation and robotization of micropropagation technology.

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Section: Explant Selectionmentioning

confidence: 98%

Environmental and Hormonal Effects in Micropropagation

Read

1992

Transplant Production Systems

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“…It would appear that the plants with ST may be somewhat similar to the tumour-prone triploid GLL, which also requires auxin for its growth (Ahuja & Hagen, 1966b). Besides, tumourprone GGLL-T stem pith tissues may also require an initial treatment with auxin to trigger habituation in culture (Cheng & Smith, 1973), compared with constitutively habituated teratomatous tissues.…”

Section: Cytologymentioning

confidence: 99%

Genetic nature of a nontumour mutant isolated from tumour-prone amphidiploid Nicotiana glauca-langsdorffii (GGLL): a critical assessment

Ahuja¹

1996

Heredity

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The genetic nature of a nontumour mutant, isolated by Izard (1957) following irradiation of germinating seeds of the tumour-prone amphidiploid Nicotiana glauca-langsdorffii (GGLL-T), is re-examined in the light of previous and current studies on tumour formation in Nicotiana hybrids. As there are lingering questions regarding the dominant nature of the nontumour trait, I shall present experimental evidence and theoretical arguments that suggest that the nontumour condition does not necessarily involve a single dominant gene mutation, but rather mutations affecting tumour expression. In the framework of a genetic model of tumour formation in Nicotiana (Ahuja, 1968), the mutations are probably in those ee loci of N glauca affecting tumour expression, and not in the I element of N langsdorffii, as previously suggested by Smith (1972) and Sekine et a!. (1993). As tumour-prone tissues are highly sensitive to environmental influences, including irradiation, it would be very difficult to obtain a mutation from tumour to a completely nontumour state, as in all likelihood, the irradiation treatments would accelerate tumour formation, or induce variation in the tumour expression. Instead, I propose a different approach for isolating a nontumour mutant, which involves irradiation of haploid cells (pollen) from N langsdorffii (I species) and then pollinating N glauca (ee species) with the irradiated pollen. As the cross N glauca x N langsdorffii normally yields a 100 per cent tumorous progeny, any deviation from this norm, i.e. a nontumour mutant, would be easily detected in the proposed cross. The reciprocal cross involving irradiated pollen from the ee parent N glauca may also be attempted to check the validity of the hypothesis.

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Adventitious Regeneration

George¹,

Hall²,

Klerk³

Plant Propagation by Tissue Culture

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The influence of genomes on autonomous growth of pith cultures of Nicotiana glauca-Langsdorffii hybrids

Cited by 12 publications

References 4 publications

Environmental and Hormonal Effects in Micropropagation

Environmental and Hormonal Effects in Micropropagation

Genetic nature of a nontumour mutant isolated from tumour-prone amphidiploid Nicotiana glauca-langsdorffii (GGLL): a critical assessment

Adventitious Regeneration

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