The Influence of Gas Saturation on Microbubble Stability

Mulvana, Helen; Stride, Eleanor; Tang, Meng‐Xing; Hajnal, Joseph V.; Eckersley, Robert J.

doi:10.1016/j.ultrasmedbio.2012.02.008

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…The same amount of stock Cy-droplet and blank-droplet emulsion was introduced into a 2L water tank (Fig. 3 (3)) filled with water and equilibrated to 37 °C [33] to achieve a final concentration of approximately 10 6 droplets/mL. Before each acquisition, the water was mixed to achieve a relatively uniform distribution of droplets.…”

Section: Methodsmentioning

confidence: 99%

Optically and acoustically triggerable sub-micron phase-change contrast agents for enhanced photoacoustic and ultrasound imaging

et al. 2017

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We demonstrate a versatile phase-change sub-micron contrast agent providing three modes of contrast enhancement: 1) photoacoustic imaging contrast, 2) ultrasound contrast with optical activation, and 3) ultrasound contrast with acoustic activation. This agent, which we name ‘Cy-droplet’, has the following novel features. It comprises a highly volatile perfluorocarbon for easy versatile activation, and a near-infrared optically absorbing dye chosen to absorb light at a wavelength with good tissue penetration. It is manufactured via a ‘microbubble condensation’ method. The phase-transition of Cy-droplets can be optically triggered by pulsed-laser illumination, inducing photoacoustic signal and forming stable gas bubbles that are visible with echo-ultrasound in situ. Alternatively, Cy-droplets can be converted to microbubble contrast agents upon acoustic activation with clinical ultrasound. Potentially all modes offer extravascular contrast enhancement because of the sub-micron initial size. Such versatility of acoustic and optical ‘triggerability’ can potentially improve multi-modality imaging, molecularly targeted imaging and controlled drug release.

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Section: Methodsmentioning

confidence: 99%

Optically and acoustically triggerable sub-micron phase-change contrast agents for enhanced photoacoustic and ultrasound imaging

et al. 2017

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“…In this study, microbubbles were diluted in gas-equilibrated water (Mulvana et al 2012) to a concentration of 2 3 10 5 microbubbles/mL, a clinically relevant concentration as used in previous studies .…”

Section: Microbubble Contrast Agentsmentioning

confidence: 99%

Flow Velocity Mapping Using Contrast Enhanced High-Frame-Rate Plane Wave Ultrasound and Image Tracking: Methods and Initial in Vitro and in Vivo Evaluation

Leow

Bazigou

Eckersley

et al. 2015

Ultrasound in Medicine & Biology

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Ultrasound imaging is the most widely used method for visualising and quantifying blood flow in medical practice, but existing techniques have various limitations in terms of imaging sensitivity, field of view, flow angle dependence, and imaging depth. In this study, we developed an ultrasound imaging velocimetry approach capable of visualising and quantifying dynamic flow, by combining high-frame-rate plane wave ultrasound imaging, microbubble contrast agents, pulse inversion contrast imaging and speckle image tracking algorithms. The system was initially evaluated in vitro on both straight and carotid-mimicking vessels with steady and pulsatile flows and in vivo in the rabbit aorta. Colour and spectral Doppler measurements were also made. Initial flow mapping results were compared with theoretical prediction and reference Doppler measurements and indicate the potential of the new system as a highly sensitive, accurate, angle-independent and full field-of-view velocity mapping tool capable of tracking and quantifying fast and dynamic flows.

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“…The water control and water bath were purified and placed a day before use to equilibrate the gas with atmospheric pressure (Mulvana et al 2012). …”

Section: Methodsmentioning

confidence: 99%

Quantifying Activation of Perfluorocarbon-Based Phase-Change Contrast Agents Using Simultaneous Acoustic and Optical Observation

Lin

et al. 2015

Ultrasound in Medicine & Biology

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Phase-change contrast agents in the form of nanoscale droplets can be activated into microbubbles by ultrasound, extending the contrast beyond the vasculature. This article describes simultaneous optical and acoustical measurements for quantifying the ultrasound activation of phase-change contrast agents over a range of concentrations. In experiments, decafluorobutane-based nanodroplets of different dilutions were sonicated with a high-pressure activation pulse and two low-pressure interrogation pulses immediately before and after the activation pulse. The differences between the pre- and post-interrogation signals were calculated to quantify the acoustic power scattered by the microbubbles activated over a range of droplet concentrations. Optical observation occurred simultaneously with the acoustic measurement, and the pre- and post-microscopy images were processed to generate an independent quantitative indicator of the activated microbubble concentration. Both optical and acoustic measurements revealed linear relationships to the droplet concentration at a low concentration range <108/mL when measured at body temperature. Further increases in droplet concentration resulted in saturation of the acoustic interrogation signal. Compared with body temperature, room temperature was found to produce much fewer and larger bubbles after ultrasound droplet activation.

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The Influence of Gas Saturation on Microbubble Stability

Cited by 24 publications

References 21 publications

Optically and acoustically triggerable sub-micron phase-change contrast agents for enhanced photoacoustic and ultrasound imaging

Optically and acoustically triggerable sub-micron phase-change contrast agents for enhanced photoacoustic and ultrasound imaging

Flow Velocity Mapping Using Contrast Enhanced High-Frame-Rate Plane Wave Ultrasound and Image Tracking: Methods and Initial in Vitro and in Vivo Evaluation

Quantifying Activation of Perfluorocarbon-Based Phase-Change Contrast Agents Using Simultaneous Acoustic and Optical Observation

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