The influence of gangliosides on the hydrophilic pore edge line tension and monolayer fusion of lipid membranes

Melikyan, Gregory B.; Matinyan, N.S.; Аракелян, В. Б.

doi:10.1016/0005-2736(90)90232-d

Cited by 18 publications

(14 citation statements)

References 15 publications

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“…In fact, difference in size between vesicles containing 20 and 30 mol% of sulfatide is negligible, as examined by elusion of the vesicles through a Sepharose CL-4B gel filtration column and electron microscopy (data not shown). On the other hand, our results did not rule out the possibility that GM 1 headgroup protrusion also played a role in inhibiting close approach and fusion of the bilayer vesicles (51,52).…”

Section: Discussioncontrasting

confidence: 94%

Ca2+-induced fusion of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles

1999

Journal of Lipid Research

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The fusogenic properties of sulfatide-containing 1,2-dioleoyl-3sn -phosphatidylethanolamine (DOPE) small unilamellar vesicles (SUVs) in the presence of CaCl 2 were studied by mixing membrane lipids based on an assay of fluorescence resonance energy transfer (FRET). Fusion of the vesicles was also confirmed by mixing aqueous contents with the Tb/dipicolinate (DPA) assay. The half-times of lipid mixing revealed that the fusion rate decreased with increasing molar concentration of sulfatide. This inhibitory effect was more obvious at sulfatide concentrations higher than 30 mol%, where hydration at the membrane surface reached its maximum and the fusion was no longer pH-sensitive in the range of pH 6.0 -9.0. Similar inhibitory effect was also observed in Ca 2 ϩ -induced fusion of DOPE/ganglioside GM 1 vesicles but at a lower concentration of the glycosphingolipid (20 mol%). In contrast, increasing the concentration of phosphatidylserine (PS) in DOPE/PS SUVs resulted in an increase in the rate of Ca 2 ؉ -induced lipid mixing and the pH sensitivity of this system was not affected. These results are consistent with an increasing steric hindrance to membrane fusion at higher molar concentration and larger headgroup size of the glycosphingolipids. Interestingly, the pH sensitivity of the sulfatide-containing liposomes was retained when they were allowed to fuse with synaptosomes in the absence of Ca 2 ؉ by a mechanism involving protein mediation. -Wu, X., and Q-T. Li. Ca 2 ؉ -induced fusion of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles.

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Section: Discussioncontrasting

confidence: 94%

Ca2+-induced fusion of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles

1999

Journal of Lipid Research

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“…Under these assumptions, the following expression was used for the line tension: γ 0 = (2 h )·(2 σ 0 ). For typical lateral tension of Mueller-Rudin membranes (black lipid films) of about 2 mN/m 31 and membrane thickness 2 h = 4–5 nm this expression yields γ 0 = 8–10 pN, which is in good agreement with the experimentally determined values for such systems 32 . However, this model predicts zero line tension at zero lateral tension, which is obviously not the case 9 , 11 , 12 .…”

Section: Resultssupporting

confidence: 84%

Pore formation in lipid membrane II: Energy landscape under external stress

Akimov

Volynsky

Galimzyanov

et al. 2017

Sci Rep

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Lipid membranes are extremely stable envelopes allowing cells to survive in various environments and to maintain desired internal composition. Membrane permeation through formation of transversal pores requires substantial external stress. Practically, pores are usually formed by application of lateral tension or transmembrane voltage. Using the same approach as was used for obtaining continuous trajectory of pore formation in the stress-less membrane in the previous article, we now consider the process of pore formation under the external stress. The waiting time to pore formation proved a non-monotonous function of the lateral tension, dropping from infinity at zero tension to a minimum at the tension of several millinewtons per meter. Transmembrane voltage, on the contrary, caused the waiting time to decrease monotonously. Analysis of pore formation trajectories for several lipid species with different spontaneous curvatures and elastic moduli under various external conditions provided instrumental insights into the mechanisms underlying some experimentally observed phenomena.

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“…Compared to pure DOPC membranes, we found a significant decrease in the edge tension, from 27.7 to 15.6 pN upon the addition of DOPE, reminding of effects induced by surfactants (21,39); see the value of γ for DOPC mixtures with the detergent Tween 20 in Table 1. Interestingly, similar values as those for γ of DOPC:DOPE 5:1 were obtained for EggPE and PE extracts from Escherichia coli (14,51). Within the framework of the membrane elasticity theory and considering the inverted-cone shape of the DOPE molecule, one would expect an increase in the edge tension upon DOPE addition, analogously to the effect of cholesterol (21).…”

Section: Discussionsupporting

confidence: 66%

“…Another way to probe the edge energy, this time on supported lipid bilayers, is provided by a "punch-through" approach with an atomic force microscope (AFM) (13), but here, the support may influence the membrane behavior. Measurements of the voltage dependence of the average lifetime of pores in electroporated black lipid mem-branes also can be used to roughly estimate the edge tension (14,15). However, these membranes are at high tension and organic solvents may still be present in the bilayer.…”

Section: Introductionmentioning

confidence: 99%

A New Method for Measuring Edge Tensions and Stability of Lipid Bilayers: Effect of Membrane Composition

Portet

Dimova

2010

Biophysical Journal

157

192

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We report a novel and facile method for measuring edge tensions of lipid membranes. The approach is based on electroporation of giant unilamellar vesicles and analysis of the pore closure dynamics. We applied this method to evaluate the edge tension in membranes with four different compositions: egg phosphatidylcholine (eggPC), dioleoylphosphatidylcholine (DOPC), and mixtures of DOPC with cholesterol and dioleoylphosphatidylethanolamine. Our data confirm previous results for eggPC and DOPC. The addition of 17 mol % cholesterol to the DOPC membrane causes an increase in the membrane edge tension. On the contrary, when the same fraction of dioleoylphosphatidylethanolamine is added to the membrane, a decrease in the edge tension is observed, which is an unexpected result considering the inverted-cone shape geometry of the molecule. It is presumed that interlipid hydrogen bonding is the origin of this behavior. Furthermore, cholesterol was found to lower the lysis tension of DOPC bilayers. This behavior differs from that observed on bilayers made of stearoyloleoylphosphatidylcholine, suggesting that cholesterol influences the membrane mechanical stability in a lipid-specific manner.

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The influence of gangliosides on the hydrophilic pore edge line tension and monolayer fusion of lipid membranes

Cited by 18 publications

References 15 publications

Ca2+-induced fusion of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles

Ca2+-induced fusion of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles

Pore formation in lipid membrane II: Energy landscape under external stress

A New Method for Measuring Edge Tensions and Stability of Lipid Bilayers: Effect of Membrane Composition

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