The influence of foreign body surface area on the outcome of chronic osteomyelitis

Surdu-Bob, Carmen Cristina; Coman, Cristin; Bărbuceanu, Florica; Turcu, D.; Bercaru, Nicolae; Bădulescu, Marius

doi:10.1016/j.medengphy.2016.04.027

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…In the control and treated group rabbits, a cotton mesh ball previously sterilized under ultraviolet radiation and soaked in the S. aureus suspension was inserted into each drill hole using a pointed tweezer as described previously. 4 A 0.2-mL aliquot of S. aureus ATCC 6538 suspension of 10 8 CFU/mL concentration was used for inoculation into each rabbit tibia. The cotton mesh balls were used to provide substrate for bacterial attachment and increase the chance of infection.…”

Section: Infection Modelmentioning

confidence: 99%

“…1,2 Local antibiotic therapy used in conjunction with systemic antibiotics emerged in the hope of better infection prevention control and treatment of bone fractures. 3,4 In terms of both effectiveness and toxicity, local antibiotic delivery systems were found to have higher potential than high-dose oral or injectable antibiotics. 5,6 Therefore, several materials and methods are being tested for local delivery: antibiotic-impregnated materials 3,7,8 (polymethyl methacrylate, ceramic, bone cement or biodegradable beads, cancellous bone, nanotubes, nanoparticles, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Copper Bead Therapy in Severe Bone Infection: A Rabbit Tibial Model

Surdu-Bob

Vlase²,

Bărbuceanu

et al. 2019

Vet Comp Orthop Traumatol

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Objective We investigated the benefits of a local preventive therapy based on copper beads against severe bone infection using a rabbit open tibial fracture model. Materials and Methods Cotton mesh balls soaked in a very high concentration of Staphylococcus aureus ATCC 6538 culture were inoculated in drilled holes of the tibiae of treated and control groups. The treated group was also implanted with small copper beads simultaneously, as prevention therapy. Results Survival rate in the treated group was 67% compared with 25% in the control group (difference 40%, for a 95% confidence interval: 40%, 93.4%). The few remaining animals in the control group had bone lesions which developed into osteomyelitis, while the tibiae of treated group had clear signs of reparatory processes. Sixty days after inoculation, signs of local-only toxicity were observed in healthy tibia of a separate non-infected control group. Drawbacks of copper toxicity were weighed against the threat of septicaemia and also against prolonged use of powerful systemic antibiotic medications in severe bone contamination. Cinical Significance It was found that the proposed therapy prevented septicaemia and the spread of infection, and it also induced reparatory processes. The findings of this study may be relevant in antisepsis of open fractures in less appropriate medical settings (such as military camps or remote locations), as well as in severe bone infections.

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Section: Infection Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Copper Bead Therapy in Severe Bone Infection: A Rabbit Tibial Model

Surdu-Bob

Vlase²,

Bărbuceanu

et al. 2019

Vet Comp Orthop Traumatol

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“…The model for induction of bone defect, osteomyelitis and disease induction is the one described previously (Coman et al, 2015;Surdu Bob et al, 2016) in short being in that way: all rabbits under total anesthesia (ketamine 35 mg/kg+xylazine 7 mg/kg, IM) were approached for left tibia inoculation, with the right remaining control. In dorsal decubitus, was choose the proximal third of the tibia, the medial-lateral face.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…(Patel et al, 2009;Lovati et al, 2017). Using a rabbit model, model described in previous papers (Coman et al, 2013;Surdu Bob et al, 2016), we have experienced alternative treatment based on silver and copper particles. The effectiveness of the treatment was evaluated based on clinical, microbiological, radiological (Coman et al, 2015) and histological signs.…”

Section: Introductionmentioning

confidence: 99%

Histological Assessment of an Experimental Model of Human Osteomyelitis in Rabbits

Coman¹,

Surdu-Bob

Bărbuceanu

et al. 2018

“Agriculture for Life, Life for Agriculture” Conference Proceedings

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The aim of this paper was to present a histological evaluation of the treatment performed on a humanmodel of osteomyelitis induced in rabbits. Osteomyelitis was induced in rabbits by a human strain of Staphylococcus aureus injected in bone defects created in the rabbit tibia. There have been created five groups of animals, groups receiving treatment in two different stages of the disease, acute and chronic. Copper and silver sub-millimetre-particles were introduced in the same place with the Staphylococcus solution. Evaluation of installation and evolution of the disease was done by clinical, hematological, microbiological, radiological and histological monitoring. A separate study of histological data is presented here. Histological examinations performed by HE was done on 5 μm sections of uncalcified bone. Every examination was classified according to a system of score. The results of histopathological evaluation confirm that histological analysis is a powerful tool in experimental models of this disease.

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“…At present, the treatment methods available for osteomyelitis mainly include surgical debridement and antibiotic treatment (Niikura et al, 2016). Surgical debridement to remove residual pathogenic bacteria in the medullary cavity is technically challenging (Surdu-Bob et al, 2016). Alternatively, the extensive and large-dose application of antibiotics may lead to the emergence of multidrug-resistant strains, further exacerbating the complexity of treating osteomyelitis caused by drug-resistant strains (Sheehy et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy Involving a Novel Photosensitizer Combined With an Antibiotic in the Treatment of Rabbit Tibial Osteomyelitis Caused by Drug-Resistant Bacteria

et al. 2022

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Osteomyelitis is deep tissue inflammation caused by bacterial infection. If such an infection persists, it can lead to dissolution and necrosis of the bone tissue. As a result of the extensive use of antibiotics, drug-resistant bacteria are an increasingly common cause of osteomyelitis, limiting the treatment options available to surgeons. Photodynamic antibacterial chemotherapy has attracted increasing attention as a potential alternative treatment. Its advantages are a broad antibacterial spectrum, lack of drug resistance, and lack of toxic side effects. In this study, we explored the impact of the new photosensitizer LD4 in photodynamic antimicrobial chemotherapy (PACT), both alone and in combination with an antibiotic, on osteomyelitis. A rabbit tibial osteomyelitis model was employed and microbiological, histological, and radiological studies were performed. New Zealand white rabbits (n = 36) were randomly divided into a control group, antibiotic group, PACT group and PACT + antibiotic group for treatment. In microbiological analysis, a reduction in bacterial numbers of more than 99.9% was recorded in the PACT group and the PACT + antibiotic group 5 weeks after treatment (p < 0.01). In histological analysis, repair of the damaged bone tissue was observed in the PACT group, and bone repair in the PACT + antibiotic group was even more significant. In radiological analysis, the X-ray Norden score showed that the severity of bone tissue defects or destruction followed the pattern: PACT + antibiotic group < PACT group < antibiotic group < control group.

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The influence of foreign body surface area on the outcome of chronic osteomyelitis

Cited by 6 publications

References 19 publications

Copper Bead Therapy in Severe Bone Infection: A Rabbit Tibial Model

Copper Bead Therapy in Severe Bone Infection: A Rabbit Tibial Model

Histological Assessment of an Experimental Model of Human Osteomyelitis in Rabbits

Antimicrobial Photodynamic Therapy Involving a Novel Photosensitizer Combined With an Antibiotic in the Treatment of Rabbit Tibial Osteomyelitis Caused by Drug-Resistant Bacteria

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