The influence of ferric (III) citrate on ATP-hydrolases  of Desulfuromonas acetoxidans ІМV В-7384

Maslovska, O.; Hnatush, S. О.

doi:10.15421/011301

Cited by 3 publications

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“…Ferrum (III) citrate at all tested concentrations in the medium represses the dissimilatory sulfur reduction carried out by bacteria, as evidenced by the decrease in the concentrations of hydrogen sulfide produced by bacteria in the media with S 0 and FeC 6 H 5 O 7 . Under these conditions of growth, ferrum (III) cations, chelated by citrate, may damage the structure of the cytoplasmic membrane of bacteria and thus influence the activity of membrane bound enzymes (Maslovska & Hnatush, 2013;Maslovska et al, 2014). Although the reduction of metalsoxidants by membrane-bound metal reductases is mainly carried out outside the cell (Gescher & Kappler, 2012;Richter et al, 2012;Simonte et al, 2017), with increase in the concentration of soluble FeC 6 H 5 O 7 in the medium the degree of Fe(III) penetration through the cytoplasmic membrane of bacteria into the cytoplasm increases, where its interaction with intracellular metabolites occurs, oxygen radicals are formed, which causes the inhibition of growth and metabolic activity of bacteria (Hnatush & Maslovska, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Sulfur reducing bacteria of the Desulfuromonas genus are obligate anaerobes, they use elemental or polysulfide sulfur, nitrates, nitrites, Lmalate, fumarate, tri-or tetrachlorethylene, oxidized forms of heavy metals as electron acceptors (Hedderich et al, 1999;Sung et al, 2003;Kuever et al, 2005;An & Picarda, 2015), oxidizing at the same time a number of simple organic compounds to CO 2 (Maslovska & Hnatush, 2013;Vasyliv et al, 2015). Sulfidogenic bacteria attract the attention of researchers as potential agents for the purification of waters contaminated with hydrogen sulfide and heavy metals.…”

Section: Introductionmentioning

confidence: 99%

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Reduction of sulfur and oxidized forms of nitrogen by bacteria of Desulfuromonas sp., isolated from Yavorivske Lake, under the influence of ferrum citrate

et al. 2020

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Technogenic reservoirs mainly contain several possible electron acceptors of anaerobic respiration, many of which are dangerous to the environment. The succession of their reduction (and thus detoxification) by sulfur reducing bacteria is not yet sufficiently studied. We investigated the influence of ferrum (III) citrate, present in the cultivation medium, on the reduction of sulfur, nitrate and nitrite ions by sulfur reducing bacteria Desulfuromonas acetoxidans IMV B-7384, Desulfuromonas sp. Yavor-5 and Desulfuromonas sp. Yavor-7, isolated from Yavorivske Lake. It was established that ferrum (III) citrate inhibits the biomass accumulation and hydrogen sulfide production by bacteria of Desulfuromonas sp. after simultaneous addition to the medium of 3.47 mM S0 and 1.74–10.41 mM ferrum (III) citrate, as compared with growth and hydrogen sulfide production by bacteria in the medium with only sulfur. In the medium with the same initial content (3.47 mM) S0 and ferrum (III) citrate bacteria produced ferrum (II) ions at concentrations 3.5–3.9 times higher than that of hydrogen sulfide. Ferrum (III) citrate inhibits the biomass accumulation, the nitrate or nitrite ions reduction and the ammonium ions production by bacteria of Desulfuromonas sp. after simultaneous addition to the medium of 3.47 mM NaNO3 or NaNO2 and 1.74–10.41 mM ferrum (III) citrate. In the medium with the same initial content (3.47 mM) NaNO3 and ferrum (III) citrate, bacteria produced ammonium ions at concentrations in 1.1 times higher than that of ferrum (II) ions. In the medium with the same initial content (3.47 mM) NaNO2 and ferrum (III) citrate, bacteria reduced 1.5–1.6 times more ferrum (III) than nitrite ions with production of ferrum (II) ions at concentrations 1.7 times higher than that of ammonium ions. The process of nitrate reduction carried out by bacteria of Desulfuromonas genus was less sensitive to the negative influence of ferrum (III) citrate, compared to the process of nitrite ions reduction. When the reduction of nitrate ions by bacteria in the presence of 1.74–10.41 mM ferrum (III) citrate decreased by 1.4–2.2 times, then the reduction of nitrite ions decreased by 1.8–3.2 times compared to their reduction in media with only NaNO3 or NaNO2, respectively. Although the reduction of ferrum (III) by cells in media with 3.47 mM S0, NaNO3 or NaNO2 and 1.74–10.41 mM ferrum (III) citrate decreased by 1.6–2.7, 1.6–2.7 and 1.1–2.2 times, respectively, compared to the reduction in medium with only ferrum (III) citrate, the investigated strains of bacteria were resistant to high concentrations of trivalent ferrum compounds and can therefore can be used in technologies of complex purification of environments polluted by heavy metal and nitrogen compounds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reduction of sulfur and oxidized forms of nitrogen by bacteria of Desulfuromonas sp., isolated from Yavorivske Lake, under the influence of ferrum citrate

et al. 2020

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“…Consequently the output of electric current decreased since the amount of electrons that could reach the anode surface diminished in comparison with the separate application of 42 mM sodium (III) citrate. It was established that D. acetoxidans IMV B-7384 growth is partly inhibited at the presence of 10 mM ferric citrate [2]. Electrons are transferred to the anode surface while bacterial oxidation of organic Carbon source in MFC if additional acceptor is not present.…”

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Electric Current Generation by Desulfuromonas Acetoxidans Imv B-7384 While Ferric Citrate, Fuchsine and Methylene Blue Application

Василів¹,

Maslovska²,

Гнатуш³

et al. 2016

Microbiology&Biotechnology

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Influence of potassium dichromate on the reduction of sulfur, nitrate and nitrite ions by bacteria Desulfuromonas sp.

Moroz

Hnatush

Yavorska

et al. 2022

Regul. Mech. Biosyst.

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This article presents the regularities of reduction of sulfur, nitrate and nitrite ions by sulfur reducing bacteria Desulfuromonas sp., which were isolated from the water of the man-made Yavorivske Lake (Lviv Region, Ukraine), under the influence of potassium dichromate. This bacteria in the process of anaerobic respiration can use and reduce different electron acceptors, such as sulfur, nitrates, nitrites, oxidized forms of heavy metals, in particular, hexavalent chromium. Technogenically altered ecotopes are characterized by complex pollution, so several electron acceptors are available to bacteria at the same time. Strains of microorganisms isolated from such ecotopes are adapted to unfavourable conditions and therefore have high biotechnological potential. The purpose of this work was to investigate the regularities of elemental sulfur, nitrate or nitrite ion usage by sulfidogenic bacteria of Desulfuromonas genus in conditions of simultaneous presence in the medium of another electron acceptor – Cr(VI), to establish the succession of reduction of electron acceptors by strains of these bacteria and to evaluate the efficiency of their possible application in technologies of complex purification of the environment from metal compounds and other inorganic toxicants. Bacteria were grown under anaerobic conditions in Kravtsov-Sorokin medium without SO42– and without Mohr’s salt for 10 days. To study the efficiency of sulfur, nitrate or nitrite ions’ reduction at simultaneous presence in the medium of Cr(VI) bacteria were sown in media with elemental sulfur, NaNO3, NaNO2 or K2Cr2O7 to final S0, NO3–, NO2–or Cr(VI) concentration in the medium of 3.47 (concentration of SO42– in medium of standard composition) or 1.74, 3.47, 5.21, 6.94 and 10.41 mM. Biomass was determined by the turbidimetric method, and the concentrations of nitrate, nitrite, ammonium ions, hydrogen sulfide, Cr(VI), Cr(ІІІ) in cultural liquid were determined spectrophotometrically. It has been established that Cr(VI) inhibits the biomass accumulation and hydrogen sulfide production by bacteria of Desulfuromonas sp. after simultaneous addition into the medium of 3.47 mM S0 and 1.74–10.41 mM Cr(VI). In the medium with the same initial content (3.47 mM) of S0 and Cr(VI) bacteria produced Cr(III) at concentrations 3.3–3.4 times higher than that of hydrogen sulfide. It has been shown that K2Cr2O7 inhibits biomass accumulation, nitrate ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO3– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO3– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) of NO3– and Cr(VI) bacteria reduced up to 1.2 times more nitrate ions than Cr(VI) with the production of ammonium ions at concentrations the same times higher than those of Cr(III). It has been established that K2Cr2O7 inhibits biomass accumulation, nitrite ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO2– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO2– and 3.47 mM Cr(VI). In the medium with the same initial content of (3.47 mM) NO2– and Cr(VI) the reduction of Cr(VI) by bacteria was only slightly, up to 1.1 times, lower than the reduction of nitrite ions, almost the same concentrations of trivalent chromium and ammonium ions were detected in the cultural liquid. The processes of nitrate and nitride reduction carried out by bacteria of Desulfuromonas genus were revealed to be less sensitive to the negative influence of sodium dichromate, as compared with the process of sulfur reduction, because in the media with the same initial content (3.47 mM) of NO3– or NO2– and Cr(VI) bacteria produced 1.1–1.2 times more NH4+ than Cr(III), but in the medium with the same initial content (3.47 mM) of S0 and Cr(VI) ) bacteria produced over than three times more Cr(III) than hydrogen sulfide. Our data allow us to conclude that bacteria of Desulfuromonas genus, the investigated strains of which are adapted to high concentrations (up to 10.41 mM) of inorganic toxicants, play an important role in the geochemical cycles of sulfur, nitrogen and chromium in aquatic environments that have been under anthropogenic influence.

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The influence of ferric (III) citrate on ATP-hydrolases of Desulfuromonas acetoxidans ІМV В-7384

Cited by 3 publications

References 0 publications

Reduction of sulfur and oxidized forms of nitrogen by bacteria of Desulfuromonas sp., isolated from Yavorivske Lake, under the influence of ferrum citrate

Reduction of sulfur and oxidized forms of nitrogen by bacteria of Desulfuromonas sp., isolated from Yavorivske Lake, under the influence of ferrum citrate

Electric Current Generation by Desulfuromonas Acetoxidans Imv B-7384 While Ferric Citrate, Fuchsine and Methylene Blue Application

Influence of potassium dichromate on the reduction of sulfur, nitrate and nitrite ions by bacteria Desulfuromonas sp.

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