The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants

Zawadzka, Marta; Orlikowska, T.

doi:10.1007/s11240-005-9063-1

Cited by 25 publications

(15 citation statements)

References 21 publications

(18 reference statements)

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“…Swartz et al (1990) reported similar rate of indirect shoot regeneration, attaining 48% in selection MD-ETCE, nevertheless this was only upon much longer period (18 weeks) of growing leaf explants on MS medium containing 1.1 mg l -1 TDZ. The superiority of TDZ for organogenesis within the Rubus genus was also reported in red raspberries (Cousineau and Donnelly 1991;Zawadzka and Orlikowska 2006).…”

Section: Discussionsupporting

confidence: 53%

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

et al. 2010

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Adventitious shoot regeneration from leaves of blackberry cultivar Č ačanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N 6 -benzylaminopurine (BAP) (2.0 mg l -1 ) or thidiazuron (TDZ) (1.0 and 2.0 mg l -1 ) alone, or either of them combined with indol-3-butyric acid (IBA), a-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l -1 ). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l -1 , applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of Č ačanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3 0 ,5,5 0 -tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection.

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Section: Discussionsupporting

confidence: 53%

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

et al. 2010

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“…It is known that raspberry shoots in vitro suffer from leaf chlorosis [39]. The main cause of chlorosis in vitro is iron deficiency.…”

Section: Discussionmentioning

confidence: 99%

Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

Lebedev

Arkaev

Dremova

et al. 2018

Plants

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Successful acclimatization and ex vitro rooting are among the key factors reducing the cost of micropropagated plants. We compared the survival of seven Russian cultivars of raspberry (Rubus idaeus) after rooting in vitro and ex vitro. Rooted shoots adapted to nonsterile conditions much better than nonrooted ones, with survival rates of 81%–98% versus 43%–76%, respectively. We studied the effects of different combinations of plant-growth regulators and gelling agents added to a proliferation medium on ex vitro rooting of primocane-fruiting raspberry cultivar “Atlant”. Reducing the agar concentration from 8 to 6.5 g/L increased the multiplication rate, but caused shoot hyperhydricity. The highest survival rate (97.2%) was observed for shoots grown in a medium containing 0.2 and 0.1 mg/L IBA, and gelled with 5 g/L agar and 0.2 g/L Phytagel. The microshoot height at the multiplication stage did not correlate with the plant growth during acclimatization. The obtained results can be used in the commercial micropropagation of the raspberry.

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“…The replacement of Fe-EDTA with Fe-EDDHA had positive effects on in vitro shoot formation of Carica papaya (Castillo et al, 1997) and Rubus spp. (Zawadzka and Orlikowska, 2006). As in Rosa hybrida 'Moneyway' (Van der Salm et al, 1994) and hybrid hazelnut 'Geneva' (Garrison et al, 2013), our study showed that Fe-EDDHA form was superior to Fe-EDTA in producing longer shoots (Fig.…”

Section: Effect Of Growth Regulators Iron Sources and Sucrose Levelsmentioning

confidence: 50%

In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Wojtania

Matysiak

2018

Folia Horticulturae

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The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied. The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.

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The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants

Cited by 25 publications

References 21 publications

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

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