The influence of experimentally induced endometritis on the PPAR expression profile in the bovine endometrium

Socha, Barbara; Łada, Piotr; Szczepańska, Alicja; Łupicka, M.; Korzekwa, Anna

doi:10.1016/j.theriogenology.2018.09.013

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…Endometritis is an inflammatory change in endometrial structure resulting from various causes (Socha and Lada, 2018). Residual tissue fragments and a fluid environment in the uterus after parturition, weakness in uterine contraction after calving, and uterine injury caused by dystocia can easily to cause infection in the uterine lumen and endometritis (Sheldon et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Zhang

Yang

et al. 2019

J. Zhejiang Univ. Sci. B

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Catalpol is the main active ingredient of an extract from Radix rehmanniae, which in a previous study showed a protective effect against various types of tissue injury. However, a protective effect of catalpol on uterine inflammation has not been reported. In this study, to investigate the protective mechanism of catalpol on lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and mouse endometritis, in vitro and in vivo inflammation models were established. The Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway and its downstream inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blot (WB), and immunofluorescence techniques. The results from ELISA and qRT-PCR showed that catalpol dose-dependently reduced the expression of pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin (IL)-1β, and IL-6, and chemokines such as C-X-C motif chemokine ligand 8 (CXCL8) and CXCL5, both in bEECs and in uterine tissue. From the experimental results of WB, qRT-PCR, and immunofluorescence, the expression of TLR4 and the phosphorylation of NF-κB p65 were markedly inhibited by catalpol compared with the LPS group. The inflammatory damage to the mouse uterus caused by LPS was greatly reduced and was accompanied by a decline in myeloperoxidase (MPO) activity. The results of this study suggest that catalpol can exert an anti-inflammatory impact on LPS-induced bEECs and mouse endometritis by inhibiting inflammation and activation of the TLR4/NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Zhang

Yang

et al. 2019

J. Zhejiang Univ. Sci. B

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“…In monogastrics this PPAR isotype is a pivotal player in adipogenesis and insulin sensitivity, but in dairy cows it has been specifically studied for its role in modulating milk fat synthesis [248][249][250], its involvement in the mTOR pathway, particularly mTORC2 [251], and its anti-inflammatory role [4,5]. All PPAR isotypes play a role in improving reproductive parameters of dairy cows, such as the mitigation of endometritis [252], contribution to folliculogenesis [253], reproductive hormone synthesis [253], and the development of embryos [254]. All PPAR isotypes were found to be upregulated in response to naturally occurring prostaglandins in bovine uterine cells [255].…”

Section: Peroxisome Proliferator-activated Receptorsmentioning

confidence: 99%

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Bionaz

Vargas‐Bello‐Pérez

Busato

2020

J Animal Sci Biotechnol

101

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High producing dairy cows generally receive in the diet up to 5–6% of fat. This is a relatively low amount of fat in the diet compared to diets in monogastrics; however, dietary fat is important for dairy cows as demonstrated by the benefits of supplementing cows with various fatty acids (FA). Several FA are highly bioactive, especially by affecting the transcriptome; thus, they have nutrigenomic effects. In the present review, we provide an up-to-date understanding of the utilization of FA by dairy cows including the main processes affecting FA in the rumen, molecular aspects of the absorption of FA by the gut, synthesis, secretion, and utilization of chylomicrons; uptake and metabolism of FA by peripheral tissues, with a main emphasis on the liver, and main transcription factors regulated by FA. Most of the advances in FA utilization by rumen microorganisms and intestinal absorption of FA in dairy cows were made before the end of the last century with little information generated afterwards. However, large advances on the molecular aspects of intestinal absorption and cellular uptake of FA were made on monogastric species in the last 20 years. We provide a model of FA utilization in dairy cows by using information generated in monogastrics and enriching it with data produced in dairy cows. We also reviewed the latest studies on the effects of dietary FA on milk yield, milk fatty acid composition, reproduction, and health in dairy cows. The reviewed data revealed a complex picture with the FA being active in each step of the way, starting from influencing rumen microbiota, regulating intestinal absorption, and affecting cellular uptake and utilization by peripheral tissues, making prediction on in vivo nutrigenomic effects of FA challenging.

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“…Although the clinical application of antibiotics in the treatment of endometritis has some efficacy, it has led to the emergence of drug-resistant strains (20). Bacteria are the main cause of endometritis, and one of the most common bacteria is E. coli (21,22). It has been reported that 15-20% of dairy cow endometritis is involved with bacterial infection, indicating that preventing and treating endometritis induced by E. coli are of great significance (23).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effect of Astragalin and Chlorogenic Acid on Escherichia coli-Induced Inflammation of Sheep Endometrial Epithelium Cells

Wang

Pan³

et al. 2020

Front. Vet. Sci.

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Endometritis is one of the main diseases which harm sheep husbandry. Astragalin and chlorogenic acid (CGA) are common active ingredients of traditional Chinese medicine (TCM) with immunoprotective, antioxidant, and anti-inflammatory properties. In the present study, sheep endometrial epithelium cells (SEECs) were successfully purified and identified, and the in vitro inflammation model of SEECs induced by Escherichia coli (E. coli) was successfully established. To explore the effect of astragalin and CGA on the inflammation induced by E. coli and its potential mechanism, six groups were set up, namely, group C, M, astragalin, CGA, BAY, and STR. Cells in group C were incubated with DMEM/F12 for 6 h, while cells in group M, astragalin, CGA, BAY, and STR were incubated with DMEM/F12, astragalin, CGA, BAY, and STR for 3 h, respectively, followed by E. coli infection at a multiplicity of infection (MOI) of 1 E. coli per cell for 3 h. Subsequently, the cells and the supernatant were collected to detect the expression of genes in the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway by ELISA, qPCR, and western blot. The results showed that E. coli could induce inflammation of SEECs in vitro, while astragalin and CGA could alleviate the inflammatory response induced by E. coli via inhibiting the activation of the TLR4/NF-κB signaling pathway, which provides a theoretical and experimental foundation for preventing sheep endometritis clinically.

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The influence of experimentally induced endometritis on the PPAR expression profile in the bovine endometrium

Cited by 11 publications

References 42 publications

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Anti-inflammatory Effect of Astragalin and Chlorogenic Acid on Escherichia coli-Induced Inflammation of Sheep Endometrial Epithelium Cells

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