The influence of experimental bioactive glasses on pulp cells behavior in vitro

Mocquot, Caroline; Colon, Pierre; Fernando, Delihta; Jackson, Phil; Pradelle-Plasse, Nelly; Grosgogeat, Brigitte; Attik, Nina

doi:10.1016/j.dental.2020.07.006

Cited by 18 publications

(19 citation statements)

References 48 publications

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“…Many proteins in the bacterial membrane are involved in cellular metabolic processes, 49 while the metabolism determines the bacterial fate by controlling the foundation of organismic vital movements such as growth and reproduction. 50 It made sense to examine the effect of compound 5j on the bacterial metabolism after cytomembrane destruction. The metabolic functions of E. coli and P. aeruginosa are measured by employing the redox indicator alamarBlue, which could be converted into pink resofurin from blue resazurin in the presence of functional enzymes in living or metabolically active cells.…”

Section: ■ Introductionmentioning

confidence: 99%

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Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

2021

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A class of quinazolone thiazoles was identified as new structural scaffolds for potential antibacterial conquerors to tackle dreadful resistance. Some prepared compounds exhibited favorable bacteriostatic efficiencies on tested bacteria, and the most representative 5j featuring the 4-trifluoromethylphenyl group possessed superior performances against Escherichia coli and Pseudomonas aeruginosa to norfloxacin. Further studies revealed that 5j with inappreciable hemolysis could hinder the formation of bacterial biofilms and trigger reactive oxygen species generation, which could take responsibility for emerging low resistance. Subsequent paralleled exploration discovered that 5j not only disintegrated outer and inner membranes to induce leakage of cytoplasmic contents but also broke the metabolism by suppressing dehydrogenase. Meanwhile, derivative 5j could intercalate into DNA to exert powerful antibacterial properties. Moreover, compound 5j gave synergistic effects against some Gram-negative bacteria in combination with norfloxacin. These findings indicated that this novel structural type of quinazolone thiazoles showed therapeutic foreground in struggling with Gram-negative bacterial infections.

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Section: ■ Introductionmentioning

confidence: 99%

“…Many proteins in the bacterial membrane are involved in cellular metabolic processes, while the metabolism determines the bacterial fate by controlling the foundation of organismic vital movements such as growth and reproduction . It made sense to examine the effect of compound 5j on the bacterial metabolism after cytomembrane destruction.…”

Section: Introductionmentioning

confidence: 99%

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

2021

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“…This slight effect on mineralization was probably due to the need of longer differentiation times, as well as the lower precision and sensitivity of this assay, in comparison with ALP measurement, as we have previously observed in other studies with mesoporous nanospheres and MC3T3-E1 pre-osteoblasts [ 53 ]. Several authors have demonstrated the pro-osteogenic effects of MBGs caused by to the release of calcium and silicon ions [ 54 ], and recent research has shown the influence of these kind of biomaterials on pulp cells behavior in vitro, increasing both ALP activity and mineralization in this cell type [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells

et al. 2021

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Due to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processes, depending on their phenotype. In this work, the action of ion release from MBG-75S, with a molar composition of 75SiO2-20CaO-5P2O5, on osteogenesis and angiogenesis and the modulatory role of macrophages have been assessed in vitro with MC3T3-E1 pre-osteoblasts and endothelial progenitor cells (EPCs) in monoculture and in coculture with RAW 264.7 macrophages. Ca2+, phosphorous, and silicon ions released from MBG-75S were measured in the culture medium during both differentiation processes. Alkaline phosphatase activity and matrix mineralization were quantified as the key markers of osteogenic differentiation in MC3T3-E1 cells. The expression of CD31, CD34, VEGFR2, eNOS, and vWF was evaluated to characterize the EPC differentiation into mature endothelial cells. Other cellular parameters analyzed included the cell size and complexity, intracellular calcium, and intracellular content of the reactive oxygen species. The results obtained indicate that the ions released by MBG-75S promote osteogenesis and angiogenesis in vitro, evidencing a macrophage inhibitory role in these processes and demonstrating the high potential of MBG-75S for the preparation of implants for bone regeneration.

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“…In order to confirm the odontogenic differentiation of hDPSCs, the marker of choice was DMP-1. DMP-1 was evaluated via immunofluorescence staining (27) .…”

Section: Discussionmentioning

confidence: 99%

Effect of Bioactive Glass Nano Particulates and Nano Hydroxyapatite Coated by Chitosan on Odontogenic Differentiation and Proliferation of human Dental Pulp Stem Cells

Ali

Mahran

Yehia

2022

Egyptian Dental Journal

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This study aimed to evaluate effect of Nano hydroxyapatite coated by Chitosan (NHAP/CS) and bioactive glass nano particulates (nBG) on odontogenic differentiation and proliferation of human dental pulp stem cells (hDPSCs).Methods: hDPSCs were seeded in direct contact with the corresponding Nano-biomaterials in 5 groups: Group (Ι): NHAP (10µg/ml), Group (ΙΙ): NHAP/CS (10µg/ml), Group (ΙΙΙ): nBG (500µg/ml). Group (IV): hDPSCs were cultured with osteogenic medium as a positive control while Group (V): hDPSCs were cultured with Dulbecco's Modified Eagle Medium (DMEM) only as a negative control. Odontogenic differentiation was evaluated based on mineralization related genes .Gene expressions were verified using alkaline phosphatase (ALP) assay and immunofluorescence staining of Dentin matrix protein (DMP-1). Proliferation of cells was evaluated via Trypan Blue staining test and methylthiazol tetrazolium (MTT) assay.Results: All the tested Nano biomaterials exhibited favorable media for odontogenic differentiation and proliferation of hDPSCs. NHAP/CS showed the highest proliferation index via MTT, highest ALP concentration and highest DMP-1Immunofluorescence staining.Conclusions: Compared to pure NHAP and a major biomaterial as nBG, NHAP/CS can induce odontogenic differentiation and proliferation of hDPSCs and could be considered as a good candidate for dentin-pulp regenerative procedures.

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The influence of experimental bioactive glasses on pulp cells behavior in vitro

Cited by 18 publications

References 48 publications

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells

Effect of Bioactive Glass Nano Particulates and Nano Hydroxyapatite Coated by Chitosan on Odontogenic Differentiation and Proliferation of human Dental Pulp Stem Cells

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